真菌遺傳研究室

• Editor:: Applied and Environmental Microbiology (2012–2017)
• Editor: Physiological and Molecular Plant Pathology
  (2014–2016)
• Editor: Austin Journal of Plant Biology (2014–present)
• Editor: Journal of Fungi (2014–present)
• Editor: Advances in Biology (2013–present)
• Editor: The Scientific World Journal-Molecular Biology
  (2013–present)
• Associate Editor, Molecular Plant-Microbe Interactions
  (2007–2009)

1. Fungal Pathogenicity
2. Plant-Fungal Interactions
3. Fungal toxins; Plant Hormones
4. Secondary Metabolites in Fungi
5. Signal Transductions in Fungi

My research centers on the understanding molecular and genetic mechanisms
involved in fungal pathogenicity or virulence. Current projects are aimed at the
investigation of the signaling pathways leading to ROS detoxification, cellular
resistance to osmotic stress and multidrug, fungicide sensitivity, spore
formation, iron acquisition and pathogenicity of the tangerine pathotype of
Alternaria alternata. Research is also concentrated on the investigation of
the biosynthetic gene clusters and pathogenicity roles of cercosporin and
elsinochrome toxins produced by Cercospora nicotianae and Esinoë
fawcettii, respectively.

上學期 :
大四-專題討論(一)
碩二-論文寫作(一)
博一-真菌遺傳學特論(全)
博一-當代植物病理學專論
博二-論文寫作(二)
下學期 :
博一-真菌遺傳學特論(全)
博二-真菌分子遺傳技術
碩博班-專題討論
國農碩-植物病理及防治　

1. Wu, J.-J., Wu, P.-C., Yago, J.I., and *Chung, K.-R., 2023.3. The regulatory hub of siderophore biosynthesis in the phytopathogenic fungus Alternaria alternata. Fungi 2023, 9, 427. https://doi.org/10.3390/ jof9040427
2. Choo, C.Y.L., Wu*, P.-C., Yago, J.I., and *Chung, K.-R., 2023.1 The Pex3-mediated peroxisome biogenesis plays a critical role in metabolic biosynthesis, stress response, and pathogenicity in Alternaria alternata. Microbiological Research 266 (2023) 127236 https://doi.org/10.1016/j.micres.2022.127236
3. Lu, H.-Y., Huang, Y.-L., Wu*, P.-C., Yago, J.I., and *Chung, K.-R., 2022.6. A zinc finger suppressor involved in stress resistance, cell wall integrity, conidiogenesis, and autophagy in the necrotrophic fungal pathogen Alternaria alternata. Microbiological Research 263 (2022) 127106 https://doi.org/10.1016/j.micres.2022.127106
4. Wu*, P.-C., Choo, C.Y.L., Lu, H.-Y., Wei, X.-Y., Chen, Y.-K., Yago, J.I., and *Chung, K.-R., 2022.7. Pexophagy is required for fungal development, resistance to hydrogen peroxide, virulence, and adaptability in Alternaria alternata. Molecular Plant Pathology. 2022; 23:1538–1554. https://doi.org/10.1111/mpp.13247
5. Chen,, Y. Cao, Y. Gai, H. Ma, Z. Zhu, K.-R. Chung and H. Li. 2021. Genome-wide identification and functional characterization of GATA transcription factor gene family in Alternaria alternata. J. Fungi 2021, 7, 1013. https://doi.org/10.3390/ jof7121013
6. Wu*, P.-C., Chen, Y.-K., Yago, J.I., and *Chung, K.-R., 2021.6. Peroxisomes implicated in the biosynthesis of siderophores and biotin, cell-wall integrity, autophagy and response to hydrogen peroxide in the citrus pathogenic fungus Alternaria alternata. Frontiers in Microbiology 12:645792. 10.3389/fmicb.2021.645792 (28 June 2021)
7. Wu*, P.-C., Chen, C.-W., Choo, C.Y.L., Chen, Y.-K., Yago, J.I., and *Chung, K.-R., 2020.10. Proper functions of peroxisomes are vital for pathogenesis of citrus brown spot disease caused by Alternaria alternata. Journal of Fungi
8. Wu, P.-C., Chen, C.-W., Choo, C.Y.L., Chen, Y.-K., Yago, J.I., and *Chung, K.-R., 2020.8. Biotin biosynthesis affected by NADPH oxidases and lipid metabolism is required for infectivity in the citrus fungal pathogen Alternaria alternata. Microbiological Research 241(2020) 126566. org/10.1016/j.micres.2020.126566
9. Fu, H., Chung, K.-R., Gai, Y., Mao, L., and Li, H. 2020b. The basal transcription factor II H subunit Tfb5 required for stress response and pathogenicity in the tangerine pathotype of Alternaria alternata. Molecular Plant Pathology 21:1337–1352. doi: 10.1111/mpp.12982
10. Fu, H., Chung, K.-R., Liu, X., and Li, H. 2020a. Aaprb1, a subtilsin-like protease, required for autophagy and virulence of the tangerine pathotype of Alternaria alternata. Microbiological Research 240 (2020) 126537. doi: 10.1111/mpp.12982
11. *Chung, K.-R. Wu, P.-C., Chen, Y.-K., and Yago, J.I. 2020. The siderophore repressor SreA maintains growth and hydrogen peroxide resistance, and cell wall integrity in the phytopathogenic fungus Alternaria alternata. Fungal Genetics and Biology 139: 103384 org/10.1016/j.fgb.2020.103384
12. Lin, Y.-C., *Chung, K.-R., and *Huang, J.-W. 2020. A synergistic effect of chitosan and lactic acid bacteria on the control of cruciferous vegetable diseases. Plant Pathology Journal (revision)
13. Chung, K.-R. Wu, P.-C., and Huang R. 2020. The SreA repressor required for growth and suppression of siderophore biosynthesis, hydrogen peroxide resistance, cell wall integrity, and virulence in the phytopathogenic fungus Alternaria alternata. (under review)
14. Yang, J.-Y., *Chung, K.-R., and *Huang, J.-W. 2020. The combined effect of Bacillus, tobacco extracts, and plant oils on the control of cruciferous vegetable anthracnose. Archives of Phytopathology and Plant Protection DOI: 10.1080/03235408.2020.1717253
15. Wang, P.-H., Wu, Pei-Ching, Huang, Richie, and *Chung, K.-R. 2020. The role of a nascent polypeptide-associated complex subunit alpha in siderophore biosynthesis, oxidative stress response and virulence in Alternaria alternata. Molecular Plant-Microbe Interactions https://doi.org/10.1094/MPMI-11-19-0315-R
16. Ma, H., Zhang, B., Gai, Y., Sun, X., *Chung, K.-R. and *Li, H. 2019. Cell-wall-degrading enzymes required for virulence in the host selective toxin-producing necrotroph Alternaria alternata of citrus. Frontier in Microbiology 10:2514. doi:10.3389/fmicb.2019.02514
17. Fan, Y.-T., *Chung, K.-R., and *Huang, J.-W. 2019. Fungichromin production by Streptomyces padanus PMS-702 for controlling cucumber downy mildew. Plant Pathology Journal 35(4): 341–
18. Kao, H.-Y., *Chung, K.-R., and *Huang, J.-W. 2019. Paraquat and glyphosate increase strawberry anthracnose severity caused by Colletotrichum gloeosporioides. Journal of General Plant Pathology 85:23–32.
19. Lin, H.-C., Yu, P.-L., Chen, L.-H., Tsai, H.-C., and *Chung, K.-R. 2018. A major facilitator superfamily transporter regulated by the stress-responsive transcription factor Yap1 is required for resistance to fungicides, xenobiotics, and oxidants and full virulence in Alternaria alternata. Frontier in Microbiology 9:2229. doi: 10.3389/fmicb.2018.02229
20. Chen, Y.-W., Satange, R., Wu, P.-C., Cyong‐Ru Jhan, C.-R., Chang, C.-K., Chung, K.-R., Waring, M. J., Lin, S.-W., Hsieh *, L.-C., Hou, M.-H. 2018. CoII(Chromomycin)2 complex induces a conformational change of CCG repeats from i-motif to base-extruded DNA duplex. International Journal of Molecular Sciences 19, 2796.
21. Huang, J.-S., Peng, Y.-H., *Chung, K.-R., and *Huang, J.-W. 2018. Suppressive efficacy of volatile organic compounds produced by Bacillus mycoides on damping-off pathogens of cabbage seedlings. Journal of Agricultural Science 156 (6), 795–809. https://doi.org/10.1017/S0021859618000746
22. Ma, H., Wang, M., Gai, Y., Fu, H., Ruan, R., *Chung, K.-R. and *Li, H. 2018. Thioredoxin and glutaredoxin systems required for oxidative stress resistance, fungicide sensitivity and virulence of Alternaria alternata. Applied and Environmental Microbiology 84 (14): e00086-18. doi: 10.1128/AEM.00086-18.
23. Fasoyina, O.E., Wang, B., Qiu, M., Han, X., Chung, K.-R., and *Wang, S. 2018. Carbon catabolite repression gene creA regulates morphology, aflatoxin biosynthesis and virulence in Aspergillus flavus. Fungal Genetics and Biology 115: 41-51.
24. Ruan, R., Wang, M., Chung, K.-R., and *Li, H. 2017. The Dsc E3 ligase complex is required for fungicide resistance and ergosterol biosynthesis in the citrus postharvest pathogen Penicillium digitatum. Microbiological Research 205: 99-106.
25. Peng, Y.-H., Yun-Jung Chou, Y.-J., Liu, Y.-C., Jen, -F., Chung, K.-R., and *Huang, J.-W. 2017. Inhibition of cucumber Pythium damping-off pathogen with zoosporicidal biosurfactant produced by Bacillus mycoides. Journal of Plant Diseases and Protection 124(5): 481-491. (September 2017)
26. Ruan, R., Wang, M., Liu, X., Sun, X., *Chung, K.-R., and *Li, H. 2017. Functional analysis of two sterol regulatory element binding proteins in Penicillium digitatum. PLoS One E 12(5): e0176485. https://doi. org/10.1371/journal.pone.0176485 (May 03)
27. Lin, T.-C., Hsieh, T.-Y., Lin, C.-L., Chung, W.-C., Chung, K.-R., and *Huang, J.-W. 2017. Pathogenic fungal protein-induced resistance and its effects on vegetable diseases. Journal of Agricultural Science 155: 1069-1081. (September 2017) doi:10.1017/S002185961700020X
28. Chen, L.-H., Tsai, -C., and *Chung, K.-R. 2017. A major facilitator superfamily transporter-mediated resistance to oxidative stress and fungicides requires Yap1, Skn7, and MAP kinases in the citrus fungal pathogen Alternaria alternata. PLoS One 12(1): e0169103. doi:10.1371/journal.pone.0169103 (January 6, 2017)
29. Lin, T.-C., Hsieh, T.-Y., Lin, C.-L., Chung, W.-C., Chung, K.-R., and Huang, J.-W. 2016. Pathogenic fungal proteins-induced resistance and its effects on vegetable diseases. Journal of Agricultural Science (revision)
30. Chen, L.-H., Tsai, H.-C., and Chung, K.-R.* 2016. A major facilitator superfamily transporter-mediated resistance to oxidative stress and fungicides requires Yap1, Skn7, and MAP kinases in the citrus fungal pathogen Alternaria alternata. PLoS One (in press)
31. Wang, M., Sun, X., Yu, D., Xu, J., Chung, K.-R., Li, H. 2016. Genomic and transcriptomic analyses of the tangerine pathotype of Alternaria alternata in response to oxidative stress. Scientific Reports 6:32437 DOI: 10.1038/srep32437
32. Yang, S.L, Yu, P.-L., and Chung, K.-R.* 2016. The glutathione peroxidase–mediated ROS resistance, fungicide sensitivity and cell wall construction in the citrus fungal pathogen Alternaria alternata. Environmental Microbiology 18: 923-935.
33. Yu, P.-L., L.-H. Chen, Chung K.-R.* 2016. How the pathogenic fungus Alternaria alternata copes with stress via the response regulators SSK1 and SHO1. PLoS One 11(2): e0149153. Doi:10.137/journal.pone.0149153
34. Ma, H., Sun, X., Wang, M., Gai, Y., Chung, K.-R., and Li, H. 2016. The citrus postharvest pathogen Penicillium digitatum depends on the PdMpkB kinase for developmental and virulent functions. International J Food Microbiology 236: 167–176.
35. Hsieh, T.-Y., Lin, T.-C., Lin, C.-L., Chung, K.-R., and Huang, J.-W. 2016. Reduction of Rhizoctonia damping-off in Chinese cabbage seedlings by fungal protein activators. Journal of Plant Medicine 58: 1–8.
36. Wang, W., Wang, M., Wang, J., Zhu, C., Chung, K.-R., and Li, H. 2016. Adenylyl cyclase is required for cAMP production, growth, conidial germination, and virulence in the citrus green mold pathogen Penicillium digitatum. Microbiological Research 192: 11-20.
37. Chang, S.-C., Deng, W.-L., Huang, H.-C., Chung, K.-R., and Tzeng, K.-C. 2016. Differential expression of pectolytic enzyme genes in Xanthomonas citri subsp. citri and demonstration that pectate lyase Pel3 is required for the formation of citrus canker. Microbiological Research 192: 1-10.
38. Chen, P.-S., Peng, Y.-H., Chung, W.-C., Chung, K.-R., Huang, H.-C., and Huang, J.-W. 2016. Inhibition of Penicillium digitatum and citrus green mold by volatile compounds produced by Enterobacter cloacae. J. Plant Pathol. Microbiol. 7: 339. doi:10.4172/2157-7471.1000339
39. Chou, C.-M., F.-Y. Yu, P.-L. Yu, R. M. Bostock, K.-R. Chung, C.-Y. Chen, J.-W. Huang, M.-H. Lee. 2015. Differential expression of five endopolygalacturonase genes and demonstration that MgPG1 overexpression diminishes virulence in the brown rot pathogen Monilina fructicola. PLoS One 10(6): e0132012. (June 29, 2015)
40. Chung, K.-R. 2014. Reactive oxygen species in the citrus fungal pathogen Alternaria alternata: The roles of NADPH oxidase. Physiological and Molecular Plant Pathology 88: 10-17. (invited review)
41. Lin, C.-H., and Chung K.-R.* 2014. Interactions of MAP kinases, histidine kinase and YAP1 in the citrus fungal pathogen Alternaria alternata. Plant Pathology Bulletin 23: 307-315.
42. Chung, K.-R., and M.-H. Lee. 2014. Split marker-mediated transformation and targeted gene disruption in filamentous fungi. In: M. van den Berg and K. Maruthachalam (eds.) Genetic Transformation System in Fungi. Springer Publishes, NY (in press) (invited contribution)
43. Tsai, H.-C., and K.-R. Chung. 2014. Calcineurin phosphatase and phospholipase C are required for developmental and pathological functions in the citrus fungal pathogen Alternaria alternata. Microbiology 160: 1453-1465.
44. Chen, L.-H., S.L. Yang and K.-R. Chung. 2014. Resistance to oxidative stress via regulating siderophore-mediated iron-acquisition by the citrus fungal pathogen Alternaria alternata. Microbiology 160: 970-979.
45. Kim, H.-K., N. Khorram, S. L. Yang, K.-R. Chung and T. A. Doherty. 2013. Impaired induction of allergic lung inflammation by Alternaria alternata mutant MAPK homologue FUS3. Experimental Lung Research 39(9): 399-409.
46. Daub, M. E., S. Herrero and K.-R. Chung. 2013. Reactive oxygen species in plant pathogenesis: the role of perylenequinone photosensitizers. Antioxidants and Redox Signaling 19: 970-989. (invited review)
47. Chung, K.-R. 2013. Mitogen-activated protein kinase signaling pathways of the tangerine pathotype of Alternaria alternata. MAP Kinase 2013; 2:e4 doi:10.4081/mk.2013.e4 (invited review)
48. Yang, S. L., and K.-R. Chung. 2013. Similar and distinct roles of NADPH oxidase components in the tangerine pathotype of Alternaria alternata. Molecular Plant Pathology 14: 543-556.
49. Chen, L.-H., C.-H. Lin, and K.-R. Chung. 2013. A nonribosomal peptide synthetase mediates siderophore production and virulence in the citrus fungal pathogen Alternaria alternata. Molecular Plant Pathology 14: 497-505.
50. Chiu, C.-M., C.-M. Chou, F.-Y. Yu, S.-M. Pan, P.-L. You, R. M. Bostock, K.-R. Chung and M.-H. Lee. 2013. Redox status-mediated regulation of gene expression and fungal virulence in the brown rot pathogen Monilina fructicola. Plant Pathology 62: 809-819.
51. Tsai, H.-C., S. L. Yang, and K.-R. Chung. 2013. Cyclic AMP-dependent protein kinase A negatively regulates conidia formation by the tangerine pathotype of Alternaria alternata. World Journal of Microbiology and Biotechnology 29: 289-300.
52. Chung, K.-R. 2012. Toxicity and biosynthesis of elsinochrome phytotoxin. Plant Pathology Bulletin 21: 221-229. (invited review)
53. Chung, K.-R. 2012. Stress response and pathogenicity of the necrotrophic fungal pathogen Alternaria alternata. Scientifica 2012, 635431
    http://dx.doi.org/10.6064/2012/635431 (invited review)
54. You, B.-J., Chang, W.-T., Chung, K.-R., Kuo, Y.-H., Yang, C.-S., Tien, N., Hsieh, H.-C., Lai, C.-C. & Lee, H.-Z., 2012. Effect of solid-medium coupled with reactive oxygen species on ganoderic acid biosynthesis and MAP kinase phosphorylation in Ganoderma lucidum, Food Research International 49: 634-640.
55. Chen, L.-H., C.-H. Lin, and K.-R. Chung. 2012. Roles for SKN7 response regulator in stress resistance, conidiation and virulence in the citrus pathogen Alternaria alternata. Fungal Genetics and Biology 49: 802-813.
56. Chung, K.-R. 2012. The non-host selective toxin cercosporin produced by Cercospora species. Plant Pathology Bulletin 21: 199-212. (invited review)
57. You, B.-J., H.-Z. Lee, K.-R. Chung, M.-H. Lee, M.-J. Huang, N. Tein, C.-W. Chen, and Y.-H. Kuo. 2012. Enhanced production of ganoderic acids and cytotoxicity by Ganoderma lucidum using solid-medium culture. Bioscience, Biotechnology, and Biochemistry 76: 1529-1534.
58. Yang, S. L., and K.-R. Chung. 2012. The NADPH oxidase-mediated production of hydrogen peroxide (H2O2) and resistance to oxidative stress in the necrotrophic pathogen Alternaria alternata of citrus. Molecular Plant Pathology 13: 900-914.
59. Graham, J.H., G.C. Colburn, K.R. Chung, and J. Cubero. 2012. Protection of citrus roots against infection by Phytophthora spp. by hypovirulent P. nicotianae is not related to induction of systemic acquired resistance. Plant and Soil 358: 39-49.
60. Liao, C.-Y., M.-Y. Chen, Y.-K. Chen, Z.-M. Sheu, K.-C. Kuo, P.-F. Chang, K.-R. Chung, and M.-H. Lee. 2012. Characterization of three Colletotrichum acutatum isolates from Capsicum spp. European Journal of Plant Pathology 133: 599–608.
61. Chen, P.-S., L.-Y. Wang, Y.-J. Chen, K.-C. Tzeng, K.-R. Chung, and M.-H. Lee. 2012. Understanding cellular defence in kumquat and calamondin to citrus canker caused by Xanthomonas citri subsp. citri. Physiological and Molecular Plant Pathology 79: 1–12.
62. Liao, C.-Y., M.-Y. Chen, Y.-K. Chen, K.-C. Kuo, K.-R. Chung, and M.-H. Lee. 2012. Formation of highly branched hyphae by Colletotrichum acutatum within the fruit cuticles of Capsicum spp. Plant Pathology 61: 262–270.
63. Yago, J.I. and K.-R. Chung. 2011. First report of twig blight disease of citrus caused by Haematonectria haematococca in Philippines. Plant Disease 95 (12): 1590
64. Lee, M.-H., S.-M. Pan, T.-W. Ng, L.-Y. Wang, K.-R. Chung. 2011. Mutations of beta tubulin codon 198 or 200 indicate resistance to thiabendazole among isolates of Penicillium digitatum collected from citrus in Taiwan. International Journal of Food Microbiology 150: 157–163.
65. Wang, N., S. L. Yang, C.-H., Lin, and K.-R. Chung. 2011. Gene inactivation in the citrus pathogenic fungus Alternaria alternata defect at the Ku70 gene associated with non-homologous end joining. World Journal of Microbiology and Biotechnology 27: 1817–1826.
66. Chung, K.-R. 2011. Elsinoë fawcettii and Elsinoë australis: the fungal pathogens causing citrus scab. Molecular Plant Pathology 12: 123–135.
67. Yago, J.I., C.-H. Lin, and K.-R. Chung. 2011. The SLT2 MAP kinase-mediated signalling pathway governs conidiation, morphogenesis, fungal virulence, and production of toxin and melanin in the tangerine pathotype of Alternaria alternata. Molecular Plant Pathology 12: 653–665.
68. Lin, C.-H., S. L. Yang, and K.-R. Chung. 2011. Cellular responses required for oxidative stress tolerance, colonization and lesion formation by the necrotrophic fungus Alternaria alternata in citrus. Current Microbiology 62: 807–815.
69. Lin, C.-H., and K.-R. Chung. 2010. Specialized and shared functions of the histidine kinase and HOG1 MAP kinase-mediated signaling pathways in the filamentous fungus Alternaria alternata of citrus. Fungal Genetics and Biology 47: 818–827.
70. Lin, C.-H., S. L. Yang, N. Wang, and K.-R. Chung. 2010. The FUS3 MAPK signaling pathway of the citrus pathogen Alternaria alternata acts independently and cooperatively with the fungal redox-responsive AP1 regulator for diverse developmental, physiological and pathogenic functions. Fungal Genetics and Biology 47: 381–391.
71. Achor, D.S., E. Etxeberris, N. Wang, S.Y. Folimonova, K.R. Chung and L.G. Albrigo. 2010. Sequence of anatomical symptom observations in citrus affected with Huanglongbing disease. Plant Pathology J. 9: 56-64.
72. Wang, N., C.-H. Lin, and K.-R. Chung. 2010. A Gα subunit gene is essential for conidiation and potassium efflux but dispensable for pathogenicity of Alternaria alternata in citrus. Current Genetics 56: 43–51.
73. Yang, S. L., and K.-R. Chung. 2010. Transcriptional regulation of elsinochrome phytotoxin biosynthesis by an EfSTE12 activator from the citrus scab pathogen Elsinoë fawcettii. Fungal Biology 114: 64–73.
74. Weiland, J. J., K.-R. Chung, and J. C. Suttle. 2010. The role of cercosporin in the virulence of Cercospora spp. to plant hosts. In: Cercospora Leaf Spot of Sugar Beet and Related Species. Ed. Lartey et al., pp. 109–117, APS Press, St. Paul, MN
75. Yang, S.-L., Lin, C.-H., and K.-R. Chung. 2009. Coordinate control of oxidative stress tolerance, vegetative growth, and fungal pathogenicity via the AP1-mediated pathway in the rough lemon pathotype of Alternaria alternata. Physiological and Molecular Plant Pathology (PMPP) 74: 100–110.
76. Chung, K.-R. 2009. Citrus diseases: ecology and control. Encyclopedia of Pest Management, 1:1, 1–5. CRC Press, Taylor & Francis. DOI: 10.1081/E-EPM-12004115 (invited review)
77. Daub, M. E., and K.-R. Chung. 2009. Photoactivated perylenequinone toxins in plant pathogenesis. In: The Mycota Volume V – Plant Relationships, 2nd Ed. Spring-Verlag Press, Berlin Heidelberg, pp. 201–218. (Chapter 11)
78. Lin, C.-H., S. L. Yang and K.-R. Chung. 2009. The YAP1 homolog–mediated oxidative stress tolerance is crucial for pathogenicity of the necrotrophic fungus Alternaria alternata to citrus. Molecular Plant–Microbe Interactions (MPMI) 22: 942–952.
79. You, B.-J., M.-H. Lee, and K.-R. Chung. 2009. Gene-specific disruption in the filamentous fungus Cercospora nicotianae using a split marker approach. Archives of Microbiology 191 (7): 615–622.