分子植物病毒研究室

1. 植物病毒分子生物學
2. 病毒鞘蛋白轉基因植物
3. 輕症病毒交護保護系統
4. 病毒診斷工具之製備

本實驗室主要研究方向為植物病毒分子生物學及以遺傳工程防治植物病毒病害，所研究的植物病毒包括蚜蟲傳播的 Potyvirus 屬中的木瓜輪點病毒 (papaya ringspot virus, PRSV)、木瓜輪點病毒西瓜型 ( PRSV W type) 與矮南瓜黃化嵌紋病毒 (zucchini yellow mosaic virus, ZYMV)，以及薊馬傳播的 Tospovirus 屬中的西瓜銀斑病毒 (watermelon silver mottle virus, WSMV)、花生環斑病毒 (groundnut ringspot virus, GRSV)、鳳仙花疽斑病毒 (impatiens necrotic spot virus, INSV)、花生黃化扇斑病毒 (peanut chlorotic fan spot virus, PCFV) 等。
其主要之病毒防治策略與研究工作大致可分為四類：

1. 病毒基因體核酸序列解析及基因功能分析；
2. 將病毒基因導入寄主植物以產生抗病毒性狀；
3. 以遺傳工程方法構築輕症病毒系統來防治強系病毒的危害；
4. 病毒診斷工具之製備包括病毒之單元與多元抗體之製備及核酸探針之開發。

1. 利用輕症病毒之交互保護作用防治木瓜輪點病毒
   木瓜輪點病毒 (PRSV) 為台灣及熱帶地區木瓜栽培之主要限制因子，葉教授利用亞硝酸突變處理獲得無病徵感染之輕症病毒，利用交互保護作用 (cross protection) 原理防治PRSV，此方法經於1985-1994年間，處理過四百餘萬棵木瓜苗木，推廣面積達二千餘公頃，維持台灣木瓜之生產，此為世界上大規模應用交互保護作用防治蚜蟲傳播病毒之首例。此成就於1988年獲美國Plant
   Disease期刊邀請作特別報導 (Feature article)，並於1994年獲Advances in Disease Vector Research邀請做review報導(Yeh et al., 1988; Yeh and Gonsalves, 1994)。
2. 木瓜輪點病毒PRSV之分子研究
   主要成就包括首先將此病毒之完整基因核酸體序列10326 nt解出，建構其基因圖譜，為當時已知植物正極性ssRNA病毒已解序者之最長者 (Yeh et al., 1992)。基於此，以T3啟動子驅動之生體外具感染力轉錄體in vitro infectious transcript及以35S promoter啟動之生體內具感染力轉錄體in vivo infectious transcript亦已構築完成 (Chiang et al., 1997; Wang et al., 1997)，此使得葉教授實驗室有能力對PRSV的任何基因做功能分析。利用強系病毒和前述輕症病毒在cDNA層次上之重組分析，已找出病原性相關之區域。目前正利用這些基礎，構築本省分離株不具病原性的遺傳工程二代疫苗供交互保護作用防治PRSV。且已解出感染木瓜最關鍵之因子為NIa-Pro基因上的一個Lys27胺基酸 (Chen et al., 2008)，葉教授實驗室於木瓜輪點病毒之分子研究已取得世界先導地位。
3. 抗病毒之基因工程作物之育成
   由PRSV病毒全長度基因體定序、病毒核鞘蛋白轉基因的構築、木瓜基因轉殖與組織培養技術平台的建立，一直到完成由政府相關單位所指定之各階段田間試驗，發展出一套非常有效的木瓜輪點病毒防治策略，並且參與各項病毒病害防治及轉基因技術的國際會議，成為國際上著名的防治木瓜輪點病毒的專家。葉教授實驗室獨力研發木瓜轉型作用，研發出幼胚再生及鞘蛋白基因農桿菌轉殖技術 (Cheng et al., 1996)，目前已有多個木瓜輪點病毒鞘蛋白轉基因木瓜株系，具備極佳抗PRSV之性狀 (Fan et al., 2009; Kung et al., 2010)，已完成溫室評估。且於1996年取得農委會田間試驗許可，已完成農試所轉基因專用試驗田進行田間評估，結果非常顯著，對木瓜產業有革命性衝擊。所有田間試驗結果已於2003年獲農委會生物安全審查委員會通過，此為我國第一宗轉基因作物田間試驗範例。而葉教授所研發之轉基因木瓜對世界各地之木瓜輪點病毒均有抗性，廣受各國重視，此方面之成就獲選為美國期刊Phytopathology (Bau et al., 2003, Jan.) 與Plant Disease (Bau et al., 2004, June) 之封面報導。 另外渠等亦已完成CMV CP轉基因菸草及番茄、TMV CP及replicase轉基因菸草及PWV CP轉基因菸草 (見著作)。目前正進行抗Tospovirus、ZYMV及PRSV-W之多重抗病性轉基因甜瓜及西瓜之構築，已有良好結果。另外於木瓜輪點病毒防治試驗當中，於田間發現另一木瓜崎葉嵌紋病毒 (Papaya leaf distortion mosaic virus, PLDMV) 可感染抗PRSV之轉基因木瓜 (Bau et al., 2008)，爲解決此問題，該研究室育成了同時具有PRSV與PLDMV之鞘蛋白基因的轉基因木瓜，並於隔離溫室接種試驗中獲得可同時抵抗PSRV與PLDMV二者之抗性 (Kung et al., 2009; 十一月封面報導)。目前已獲得農委會的許可，進行田間試驗。此具備雙重抗病毒性狀木瓜之成果，已穩居世界領先之位置。
4. Tospovirus病毒屬之研究
   近年來台灣西瓜及其他瓜類廣受由薊馬傳播之病毒危害，此病毒經分離鑑定及血清比對，證實和Tospovirus屬中已知病毒不同。葉教授進一步完成此病毒之nucleocapsid (N) gene之分析比對，確認此為Tospovirus之新病毒，並命為西瓜銀斑病毒 (Watermelon silver mottle virus, WSMoV) (Yeh and Chang, 1995)。此病毒之全長度S RNA (Yeh et al., 1996) 及M RNA (Chu and Yeh, 1998) 之全長序列分析均已完成，其N-gene轉基因菸草及番茄亦已育成。除WSMoV外，該實驗室目前尚有落花生分離株及蝴蝶蘭分離株均具血清特異性為二可能新種 (Yeh et al., 1996; Chen et al., 2005)。實驗室目前並自行製備世界上最齊全之serogroup I-VI之各種單元及多元抗體，已成為全球Tospovirus屬病毒鑑定及分類重鎮。由於在Tospovirus屬上之優異成就，葉教授於1995年獲選為國際病毒Tospovirus study group國際七名委員之一成員，為個人及國家之光榮。在國際上對於此屬病毒的研究已佔有首席之地，渠並於2003年獲國際重要書刊Advances in Plant Disease Management之邀撰寫專章「Occurrence, Importance, Taxonomy and Control of Thrips-borne Tospoviruses」，可見其成就非凡。目前已將WSMoV中L RNA之高保留性序列以不同之構築形式轉殖於菸草，可獲得具有廣效性對抗兩種不同tospoviruses的轉基因植株，且抗性表現良好。菸草模式建立後，目前正努力育成轉基因番茄，以期能實際應用於經濟作物以防治熱帶及亞熱帶tospoviruses所造成的嚴重經濟損失。葉教授在tospoviruses的偵測檢疫工作上成果輝煌，如多元或單株抗體之製備 (Chen et al., 2006，中華民國發明專利第I301853號)，以及專一性或簡併性引子對之開發等 (Chu et al., 2001a & b)，可針對tospoviruses的多數病毒或是個別血清型的病毒進行快速暨準確之偵測及分類。這些成果皆有助於對田間或自國外進口的植物檢體提供良好的鑑定依據，在防檢疫及防治的工作上無疑是不可或缺的利器。
5. 植物病毒載體之開發利用
   葉教授亦對植物病毒載體之建構投入不少心力，將分離自田間罹病甜瓜之矮南瓜黃化嵌紋病毒 (Zucchini yellow mosaic virus, ZYMV) 基因體定序後，開發成病毒載體，可於感染後在植物體內大量表現水母綠色螢光蛋白GFP、塵螨過敏原Der p 5，以及另一感染瓜類之西瓜銀斑病毒 (Watermelon silver mottle virus, WSMoV) 之核鞘蛋白 (nucleocapsid protein, NP) 與非結構性NSs蛋白。所表現之蛋白經Ni2+-NTA親和性管柱純化後，可做為抗血清或單株抗體製備之用。此外，於動物試驗中證實小白鼠餵食含有ZYMV表現之塵螨過敏原Der p 5的植物材料後，能有效地降低過敏反應的指數，該成果亦於國際免疫學期刊中發表 (Hsu et al., 2004，中華民國發明專利第I288778號)。另一方面，以定點突變方式所產生的弱系ZYMV載體則具有在系統性寄主上僅產生輕微病徵且於單斑寄主無局部病斑形成之特性，相當適合做為研究植物交互保護及病毒基因與植物間交互作用關係之用 (Lin et al., 2007; 三月封面報導)。此外，以開發ZYMV病毒載體之經驗，亦將分離自彩色海芋之蕪菁嵌紋病毒 (Turnip mosaic virus, TuMV) 建構成為另一植物病毒載體，並成功地表現GFP、Der p 5以及WSMoV之NP與NSs等蛋白，經定點突變之弱系TuMV亦能對野生型病毒提供良好的交互保護抗性 (Chen et al., 2007)。

植物病毒學、植物生物科技專題介紹、論文寫作(一)、分子植物病毒學、分子病毒學文獻選讀、論文寫作、植病文獻選讀

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3. Huang, C.-H., Hsiao, W.-R., Huang, C.-W., Chen, K.-C., Lin,
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    Nucleotide sequence analysis and detection application of the
    coat protein gene of Sweet potato latent virus isolated from
    central Taiwan. Plant Pathology Bulletin 16, 141-148.
40. Chen, C.-C., Chen, T.-C., Raja, J. A. J., Chang, C.-A.,
    Chen, L.-W., Lin, S.-S. & Yeh, S.-D. (2007). Effectiveness and
    stability of heterologous proteins expressed in plants by Turnip
    mosaic virus vector at five different insertion sites. Virus
    Research 130, 210-227.
41. Chiang, C.-H., Lee, C.-Y., Wang, C.-H., Jan, F.-J., Lin,
    S.-S., Chen, T.-C., Raja, J. A. J. & S.-D., Y. (2007). Genetic
    analysis of an attenuated Papaya ringspot virus strain applied
    for cross protection. European Journal of Plant Pathology 118,
    333-348.
42. Lin, S.-S., Wu, H.-W., Jan, F.-J., Hou, R. F. & Yeh, S.-D.
    (2007). Modifications of the HC-Pro of Zucchini yellow mosaic
    potyvirus for generation of attenuated mutants for cross
    protection against severe infection. Phytopathology 97, 287-296.

1. Yeh, S.-D., Kung, Y.-J., Bau, H.-J., Yu, T.-A. & Raja, J. A. J. (2010).
   Generation of a Papaya Hybrid Variety with Broad-Spectrum Transgenic Resistance
   to Papaya ringspot virus and Papaya leaf-distortion mosaic virus. Transgenic
   Plant Journal 4, 37-44.
2. Lo, C.-C., Bau, H.-J., Chen, S.-C., Lin, C.-J., Huang, Y.-C. &
   Yeh, S.-D.
   (2010). Persistence and Bioavailability of Transgenic Genes Released from
   Genetically Modified Papaya and the Influence on Soil Bacterial Communities in
   Taiwan. Transgenic Plant Journal 4, 77-89.
3. Hsu, H.-T., Chen, T.-C., Chang, C.-A. & Yeh, S.-D. (2009). Chapter 4:
   Preparation of immunogens and production of antibodies. Methods Mol Biol. 508,
   37-50.
4. Yeh, S.-D., Bau, H.-J., Kung, Y.-J. & Yu, T.-A. (2007). Chapter I.4. Papaya.
   In Biotechnology in Agriculture and Forestry. Volume 60: Transgenic Crops V.
   73-96. Edited by E. C. Pua and M. R. Davery, Springer-Verlag, Berlin Heidelberg.
5. Yeh, S.-D. (2007). Current status of the transgenic approach for control of
   Papaya ringpsot virus. In: Business Potential for Agricultural Biotechnology
   Products, 58-66. Edited by Paul S. Teng. Asian Productivity Organization. Tokyo.

1. Lin, W. -Y, Chen, K. -C., Yang, C. -F., Uthaman Yazhisai,
   Yeh, S.-D. 2015. Generation of transgenic tomato lines with
   broad-spectrum resistance to tospoviruses and begomoviruses.
   Annual meeting of Taiwan Phytopathology Society. Abstract, SCB18
   (National Chung Hsing University, Taichung, Mar. 27-28, 2015)
2. Yeh, S.-D., Chen, T. -C., Peng, J. -C., & Yazhisai Uthaman.
   2015. Diversity of Tospoviruses in Asia, and Possible Control by
   Broad-spectrum Transgenic Resistance. (Keynote Speech, Xth
   International Symposium on Thysanoptera and Tospoviurses,
   Monterey, California, USA, May. 16-20, 2015)
3. Chen, T. -C., Chien, W. -C., Tsai, W. -T., Kang, Y.-C.,
   Chen, Y.-H., Wang, Y. -C., Ou, Y. -J., & Yeh, S.-D. 2015. An
   efficient serological platform for identification and detection
   of tospoviral species prevalent in different geographic regions.
   (Oral Presentation, Xth International Symposium on Thysanoptera
   and Tospoviurses, Monterey, California, USA, May. 16-20, 2015)
4. Yeh, S.-D., Kung, Y.-J., You, B.-J., Chen, K.-C., Bau,
   H.-J., Yang, J.-F., and Huang C.-H. 2015. Homology-independent
   breakdown of transgenic resistance by more virulent virus strain
   and the potential solution. (Keynote Speech, 11th Cross Strait
   Conference on Plant Molecular Biology and Plant Biotechnology,
   Wuhan, China, June 23-25, 2015)
5. Yeh, S.-D. 2015. Nucleotide Sequence-Homology-independent
   Breakdown of Transgenic Resistance by More Virulent Virus Strain
   and the Solution. (Keynote Speech, Guangxi University – Chung
   Hsing University on Agricultural and Biological Science
   Symposium, Guangxi, China, Oct. 27-31, 2015)
6. Yeh, S.-D., Chang, C.-P., Chutirat Assawathep, Srimek
   Chowpongpang and Wichai Cositratana. 2015. Progress for control
   of Papaya ringspot virus inThailand by cross protection.
   (Keynote Speech, International Symposium for Agricultural
   Biotechnology, Thailand, Dec.16-20, 2015)
7. Kang, Y.-C., Chen, T.-C., Yeh, S.-D. 2015. Study on the gene
   silencing suppressor and pathogenicity determinant of Cucurbit
   chlorotic yellows virus. PV-07. (Kaohsiung District Agricultural
   Research and Extension Station, Pingtung, Taiwan, Dec. 25, 2015)
8. Chien, W.-C.‚ Li, J.-T., Yang, C.-F., Yeh, S.-D. and Chen,
   T.-C. 2015. Generation of transgenic Nicotiana benthamiana
   plants conferring resistance against Cucurbit chlorotic yellows
   virus. PV-11. (Kaohsiung District Agricultural Research and
   Extension Station, Pingtung, Taiwan, Dec. 25, 2015)
9. Yeh, S.-D., Lin, S.-S., and Chua, N.-H. 2007. A novel
   strategy for plant virus resistance using artificial miRNA. 2007
   International Symposium on Biocatalysis and Biotechnology,
   Abstract, p. 44 (National Chung Hsing University, Taichung,
   November 28-30, 2007)
10. Ho, H.-Y., Ku, H.-M., Yu, T.-A., Yeh, S.-D., and Jan,
    Fuh-Jyh. 2007. Development of transgenic watermelon plants with
    multiple resistance to Cucumber mosaic-、Cucumber green mottle
    mosaic- and Watermelon mosaic viruses . 2007 International
    Symposium on Biocatalysis and Biotechnology. (National Chung
    Hsing University, Taichung, November 28-30, 2007)
11. 彭瑞菊、鄭安秀、葉錫東。2008。2007年初台南地區洋香瓜病毒病害嚴重發生原因之探討。Annual meeting
    of Taiwan Phytopathology Society, Abstract, V-6 (National Chung
    Hsing University, Taichung, Jan. 25, 2008)
12. 陳金枝、江芬蘭、陳宗祺、葉錫東、黃春惠、鄭櫻慧、張清安。2008。Capsicum chlorosis
    virus感染孤挺花之血清學及分子生物特性之鑑定。Annual meeting of Taiwan Phytopathology
    Society, Abstract, V-10 (National Chung Hsing University,
    Taichung, Jan. 25, 2008)
13. 陳金枝、黃春惠、陳宗祺、葉錫東、鄭櫻慧、張清安。2008。WsMoV感染海芋之血清學及分子生物特性之鑑定。Annual
    meeting of Taiwan Phytopathology Society, Abstract, V-11
    (National Chung Hsing University, Taichung, Jan. 25, 2008)
14. 彭瑞菊、陳紹崇、葉錫東。2008。番茄種子種苗帶病毒率檢測及發展番茄種子去病毒技術之研究。Annual meeting
    of Taiwan Phytopathology Society, Abstract, V-16 (National Chung
    Hsing University, Taichung, Jan. 25, 2008)
15. Dmytro, U., Chen, K. C. and Yeh, S. D. 2008. Construction of
    artificial miRNA for generating transgenic resistance against
    potyviruses in plant. Annual meeting of Taiwan Phytopathology
    Society, Abstract, SC-02 (National Chung Hsing University,
    Taichung, Jan. 25, 2008)
16. Wang, P. T., Chang, Y. L., Chiang, C. H., Chen, Y. T., Shaw,
    S. F., Yeh, S. D., Shih, H. D. and Yu, T. A. 2008. Transgenic
    melons expressing antifungal protein (AFP3) conferred resistance
    against Rhizoctonia solani. Annual meeting of Taiwan
    Phytopathology Society, Abstract, SC-03 (National Chung Hsing
    University, Taichung, Jan. 25, 2008)
17. Chen, J. H., Li, C. M., Chiang, C. H., Yeh, S. D. and Yu, T.
    A. 2008. Double resistance of transgenic watermelon expressing
    an untranslatable chimeric construct carrying parts of coat
    protein coding sequence of Zucchini yellow mosaic virus and
    Papaya ringspot virus type-W. Annual meeting of Taiwan
    Phytopathology Society, Abstract, SC-04 (National Chung Hsing
    University, Taichung, Jan. 25, 2008)
18. Wu, H. W., Lin, S. S., Chen, K. C., Yeh, S. D., Chua, N. H.
    2008. Discriminating mutations of HC-Pro with differential
    effects on miRNA and siRNA pathwas. International Union of
    Microbiological Societies 2008-XIV International Congress of
    Virology, Abstract, VOP-37, p. 28 (Istanbul, Turkish, Aug.
    10-15, 2008)
19. Chen, C. C., Yeh, S. D. 2008. Expression of dust mite
    allergen in edible leaves of crucifers by Turnip mosaic virus
    vector. International Union of Microbiological Societies
    2008-XIV International Congress of Virology, Abstract, VOP-229,
    p.142 (Istanbul, Turkish, Aug. 10-15, 2008)
20. Chen, K. C., Chiang, C. H., RAJA, J. A. J., Liu, F. L., Tai,
    C. H., Yeh, S. D. 2008. A signal amino acid of NIaPro of papaya
    ringspot virus determining host specificity for infection of
    papaya. International Union of Microbiological Societies
    2008-XIV International Congress of Virology, Abstract, VP-316,
    p.336 (Istanbul, Turkish, Aug. 10-15, 2008)
21. Chen, C. C., Chiang, F. L., Chen, T. C., Yeh, S. D., Cheng,
    Y. H., and Chang, C. A. 2009. Serological and molecular
    identification of Capsicum chlorosis virus infecting blood lily.
    Annual meeting of Taiwan Phytopathology Society, Abstract, V04
    (National Chung Hsing University, Taichung, Feb. 6, 2009)
22. Yeh, Y. C., Lin, Y. P., Yeh, S. D., Chen, T. C. 2009. Quick
    detection of tospoviruses using degenerate primers. Annual
    meeting of Taiwan Phytopathology Society, Abstract, SC25
    (National Chung Hsing University, Taichung, Feb. 6, 2009)
23. Kang, Y. C., Yeh, S. D., Chen, T. C. 2009. Production of
    broad-spectrum antisera for detection of tospoviruses. Annual
    meeting of Taiwan Phytopathology Society, Abstract, SC27
    (National Chung Hsing University, Taichung, Feb. 6, 2009)
24. Li, J. X., Cheng, H. W., Yeh, S. D. 2009. Enhancement of the
    production of recombinant proteins expressed by Zucchini yellow
    mosaic virus vector through fusion with the nucleocapsid protein
    of a tospovirus. Annual meeting of Taiwan Phytopathology
    Society, Abstract, SC30 (National Chung Hsing University,
    Taichung, Feb. 6, 2009)
25. Yang, J. F., Yeh, S. D., Chiang, C. H., Yu, T. A. 2009.
    Transgenic watermelon lines with triple-virus resistance
    generated by Agrobacterium-mediated transformation. Annual
    meeting of Taiwan Phytopathology Society, Abstract, SC33
    (National Chung Hsing University, Taichung, Feb. 6, 2009)
26. Kung, Y.-J., Lin, S.-S., Huang, Y.-L., Chua, N.-H. and Yeh,
    S.-D. 2009. Artificial microRNAs targeting the conserved motifs
    of replicase gene conferring transgenic resistance to
    negative-sense ssRNA plant virus. International Symposium on
    Vascular Biology and Agriculture (Chungqing, China, Jun. 21-24,
    2009)
27. Yeh, S.-D. and Chen, T.-C. 2009. Detection and
    identification of thrips-borne tospoviruses by serological and
    nucleic acid tools. APSA-AVRDC Workshop on Tospoviruses and
    Thrips Vectors, Asian Seed Congress 2009 (Bangkok, Thailand,
    Nov. 8, 2009)
28. Cheng, Y. F., Li, J. T., Chen, L. F., Huang, L. H., Peng, J.
    C., Yeh, S. D., and Chen, T. C. 2009. Evaluation of field
    surveys for tospoviruses and potyviruses in the cucurbit-growing
    areas of central and southern Taiwan . Annual meeting of Taiwan
    Plant Protection Society, Abstract, PP-13, p. 39 (Agricultural
    Chemicals and Toxic Substances Research Institute, Taichung,
    Nov. 20, 2009)
29. Ho, C. Y., Li, J. T., Kang, Y. C., Chen, Y. T., Fan, Y. S.,
    Huang, L. H., Tseng, H. H., Yeh, S. D., and Chen, T. C. 2009.
    Emergence of Tomato spotted wilt virus infects Capsicum spp. And
    Zantedeschia sp. crops in Taiwan . Annual meeting of Taiwan
    Plant Protection Society, Abstract, PP-14, p. 40 (Agricultural
    Chemicals and Toxic Substances Research Institute, Taichung,
    Nov. 20, 2009)
30. Lin, B. Y., Chen, K. C., Yeh, S. D., Yu, T. A., Chiang, C.
    H. 2010. The infectivity assays of Papaya ringspot virus
    contained the mutation at the dual coat protein cleavage sites.
    Annual meeting of Taiwan Phytopathology Society, Abstract, SC27
    (National Taiwan University, Taipei, Mar. 6, 2010)
31. Fan, Y. H.,Yeh, Y. C., Yeh, S. D., Chen T. C. 2010.
    Determination and molecular analyses of the NSm genes of Tomato
    yellow ring virus and Peanut chlorotic fan-spot virus. Annual
    meeting of Taiwan Phytopathology Society, Abstract, SC28
    (National Taiwan University, Taipei, Mar. 6, 2010)
32. Tsai, W. T., Yeh, S. D. 2010. Production of polyclonal and
    monoclonal antibodies against the NSs proteins of
    Euro-America-Type tospoviruses. Annual meeting of Taiwan
    Phytopathology Society, Abstract, SC30 (National Taiwan
    University, Taipei, Mar. 6, 2010)
33. Peng, J. C., Chen, S. K.,Chang, Y. L., Tsai, H. Y., Hu, C.
    C., Yeh, S. D. 2010. The relationship between the retention
    virus rate in whitefly and the Squash leaf curl virus disease
    occurred on melon plants at Tainan area. Annual meeting of
    Taiwan Phytopathology Society, Abstract, V07 (National Taiwan
    University, Taipei, Mar. 6, 2010)
34. Kung, Y. J., Lin, S. S., Huang, Y. L., Chen, T. C., Chua, N.
    H., and Yeh, S. D. 2010. Artificial microRNAs targeting the
    conserved motifs of replicase gene conferring transgenic
    resistance to negative-sense ssRNA plant virus. The 12th World
    Congress of the IAPB (St. Louis, Missouri, USA, Jun. 9-11, 2010)
35. Huang, C.-H., Hsiao, W.-R., Wang, N.-Y., Huang, C.-W. and
    Yeh, S. D. 2010. Analysis of essential regions of the NSs
    protein of Watermelon silver mottle virus for pathogenicity by
    attenuated strain of Zucchini yellow mosaic virus with point
    mutated in HC-Pro gene. The 12th World Congress of the IAPB (St.
    Louis, Missouri, USA, Jun. 9-11, 2010)
36. Yang, C.-F., Yu, T.-A., Yeh, S.-D. 2010. Generation of
    transgenic watermelon resistant to Zucchini yellow mosaic virus
    and Papaya ringspot virus type W. The 12th World Congress of the
    IAPB (St. Louis, Missouri, USA, Jun. 9-11, 2010)
37. Wu, P. R., Kang, Y. C., Li, J. T., Yeh, S. D., and Chen, T.
    C. 2010. Nucleotide sequencing of S RNA of Chrysanthemum stem
    necrosis virus and expression of its nucleocapsid protein for
    antiserum production. Annual meeting of Taiwan Plant Protection
    Society, Abstract, PP-14, p. 47 (National Pingtung University of
    Science and Technology, Pingtung, 10 December 2010).
38. Chiu, W. C., Kang, Y. C., Chen, S. M., Chan, S. F., Yeh, S.
    D., and Chen, T. C. 2010. Production of the nucleocapsid
    protein-based antisera for detection of tospoviruses belonging
    to TSWV serogroup. Annual meeting of Taiwan Plant Protection
    Society, Abstract, PP-15, p. 48 (National Pingtung University of
    Science and Technology, Pingtung, 10 December 2010).
39. Hsia, C. M., Chan, S. F., Kang, Y. C., Li, J. T., Yeh, S.
    D., and Chen, T. C. 2010. Production of a monoclonal antibody
    against the nucleocapsid protein of Groundnut ringspot virus.
    Annual meeting of Taiwan Plant Protection Society, Abstract,
    PP-16, p. 49 (National Pingtung University of Science and
    Technology, Pingtung, 10 December 2010).
40. Wu, H. W., Lin, S. S., Chen, K. C., Huang, C. H., Chua, N.
    H. and Yeh, S. D. 2011. The roles of potyviral gene silencing
    suppressor on symptom severity and host defensive responses.
    Annual meeting of Taiwan Phytopathology Society, Abstract, KS02
    (Chaoyang University of Technology, Taichung, Mar. 25-26, 2011)
41. Uthaman, Y., Chen, K. C., Huang, Y. L. Rajagopalan, P. A.,
    Chen. T. C. and Yeh, S. D. 2011. Generation of marker-free
    transgenic plants with broad-spectrum resistance to different
    tospoviruses. Annual meeting of Taiwan Phytopathology Society,
    Abstract, V06 (Chaoyang University of Technology, Taichung, Mar.
    25-26, 2011)
42. Hsia, C. M., Chan, S. F., Kang, Y. C., Li, J. T., Yeh, S. D.
    and Chen, T. C. 2011. Epitope analysis of a monoclonal antibody
    broadly reacted with the nucleocapsid proteins of
    Euro-American-type tospoviruses. Annual meeting of Taiwan
    Phytopathology Society, Abstract, V11 (Chaoyang University of
    Technology, Taichung, Mar. 25-26, 2011)
43. Wu, C. Y., Korniichuk, I. and Yeh, S. D. 2011. Generation of
    transgenic tobacco plants carrying the conserved regions of
    HC-Pro, CIP, NIb and CP genes for broad-spectrum resistance
    against potyviruses. Annual meeting of Taiwan Phytopathology
    Society, Abstract, SC20 (Chaoyang University of Technology,
    Taichung, Mar. 25-26, 2011)
44. Maulidiyah, A. Shen, T. H. and Yeh, S. D. 2011.
    Broad-spectrum transgenic resistance against tospoviruses
    conferred by the expression of a single-chain antibody against
    the common epitope of the gene silencing suppressor. Annual
    meeting of Taiwan Phytopathology Society, Abstract, SC22 (Chaoyang
    University of Technology, Taichung, Mar. 25-26, 2011)
45. Yeh, S. D., Chen, K. C. and Cheng, H. W. 2011. A critical
    amino acid of 6K2 protein of Papaya ringspot virus for inducing
    wilting symptom on papaya plants. 2011 APS-IPPC Joint Meeting,
    Abstract, P994. (Honolulu, Hawaii, USA, Aug. 6-10, 2011)
46. Peng, J. C., Chen, T. C. and Yeh, S. D. 2011. Generation of
    broad-spectrum resistance in transgenic tobacco and tomato
    plants against distinct tospovirus species of different
    serogroups. 2011 APS-IPPC Joint Meeting, Abstract, P685.
    (Honolulu, Hawaii, USA, Aug. 6-10, 2011)
47. Yeh, S. D. and Kung, Y. J. 2011. Solving the problem of
    sequence homology-independent breakdown of transgenic resistance
    by disarming viral gene silencing suppressor. 2011 APS-IPPC
    Joint Meeting, Abstract, P995. (Honolulu, Hawaii, USA, Aug.
    6-10, 2011)
48. Chiang, C. H., Wu, Z. J., Lin, B. Y., Bau, H. J., Chen, K.
    C., Yu, T. A. and Yeh, S. D. 2011. Deletion of the N terminus of
    Papaya ringspot virus larger coat protein disrupt viral systemic
    infection. 2011 APS-IPPC Joint Meeting, Abstract, P998.
    (Honolulu, Hawaii, USA, Aug. 6-10, 2011)
49. Yeh, S. D., Chen, C. C., Raja, J. A. J. and Huang, C. H.
    2011. Cross-protection effectiveness of attenuated variants of
    Turnip mosaic virus with mutations in a conserved motif of the
    N-terminal region of HC-Pro. International Union of
    Microbiological Societies 2011-XV International Congress of
    Virology, Abstract, VI-SY23-6. (Sapporo, Hokkaido, Japan, Sep.
    11-16, 2011)
50. Chen, T. C., Li, J. T., Huang, L. H., Weng, J. S., Cheng, Y.
    F., Peng, C. F. and Yeh, S. D. 2011. Host preference of
    Watermelon silver mottle virus and Melon yellow spot virus for
    field watermelon and melon. International Union of
    Microbiological Societies 2011-XV International Congress of
    Virology, Abstract, VI-SY46-6. (Sapporo, Hokkaido, Japan, Sep.
    11-16, 2011)
51. Cheng, H. W., Li, J. S., Chen, K. C. and Yeh, S. D. 2011.
    Development of a common epitope of NSs protein of Asia-type
    tospoviruses as a tag for recombinant proteins expressed in
    bacterial and plant viral systems. International Union of
    Microbiological Societies 2011-XV International Congress of
    Virology, Abstract, VI-SY49-3. (Sapporo, Hokkaido, Japan, Sep.
    11-16, 2011)
52. Huang, C. H., Chen, K. C., Wu, H. W., Hsiao, W. R. and Yeh,
    S. D. 2011. Analysis of essential regions of NSs protein of
    Watermelon silver mottle virus for gene silencing suppression.
    International Union of Microbiological Societies 2011-XV
    International Congress of Virology, Abstract, VI-PO26-2.
    (Sapporo, Hokkaido, Japan, Sep. 11-16, 2011)
53. Yeh, S. D., Chen, T. C. and Peng, J. C. 2011. Broad-spectrum
    transgenic resistance against different tospoviruses at the
    genus level conferred by the conserved region of L genes.
    International Union of Microbiological Societies 2011-XV
    International Congress of Virology, Abstract, VI-PO23-6.
    (Sapporo, Hokkaido, Japan, Sep. 11-16, 2011)
54. Kang, Y. C., Tsai, W. T., Haung, C. H., Li, J. T., Weng, J.
    S., Yeh, S. D. and Chen, T. C. 2011. Prompt identification of
    Asian-type and Euroamerican-type tospoviruses using monoclonal
    antibodies against the common epitopes of NSs proteins.
    International Union of Microbiological Societies 2011-XV
    International Congress of Virology, Abstract, VI-PO46-4.
    (Sapporo, Hokkaido, Japan, Sep. 11-16, 2011)
55. Kung, Y. J., You, B. J., Chen, K. C., Huang, C. H., Bau, H.
    J. and Yeh, S. D. 2011. The breakdown of transgenic resistance
    of papaya by super strains of Papaya ringspot virus and the
    solution. 3rd The International Symposium on Papaya, Keynote
    speaker (Chiang Mai, Thailand, Dec. 19-21, 2011)
56. Yeh, S. D. 2012. New technology for plant virus control. The
    International Conference on Tropical and Sub-tropical Plant
    Diseases 2012, Keynote speaker (Chiang Mai, Thailand, Feb. 7-10,
    2012)

葉錫東、鄭浩文。真核生物表現系統及其應用。中華民國專利第I 504747號(2015/10/21- 2033/08/07)。