植物病原細菌暨微生物生態研究室

1. 植物病原性細菌
2. 微生物生態
3. 難養細菌相關病害

1. Reun-Ping Goh*, Shin Lee*, Zi-Qing Fang*, Wen-Chien Tang* and Chia-Ching Chu#. 2024. First report of Pseudomonas cichorii causing bacterial leaf blight of pocketbook plant (Calceolaria hybrida) in Taiwan. Plant Disease. (SCI; Accepted) 
2. Wen-Chien Tang, Liang-Hsuan Wang, Jiun-Jie Chan, Reun-Ping Goh, Yea-Fang Wu and Chia-Ching Chu#. 2024.  Inter- and intra-specific variations in phenotypic traits of Pectobacterium strains isolated from diverse eudicots and monocots in Taiwan. Plant Disease. (SCI; https://doi.org/10.1094/PDIS-10-23-2130-RE)
3. Reun-Ping Goh*, Shin Lee*, and Chia-Ching Chu#. 2024. First report of a ‘Candidatus Phytoplasma australasiaticum’-related phytoplasma strain associated with shoot proliferation disease of variegated croton in Taiwan. Plant Disease 108(3):781. (SCI)
4. Ru-Bin Yao, Xiao-Hua Yan, Hsi-Ching Yen, Shin Lee, Chia-Ching Chu, Cheng-Fang Hong#, Chih-Horng Kuo#. 2024. Complete genome sequence of Agrobacterium pusense Bbcg2-2, a strain isolated from blueberry crown gall in Taiwan. Microbiology Resource Announcements. 13(2):e01083-23. (SCI)
5. Zi-Qing Fang, Yi-Chang Liao, Shin Lee, Man-Miao Yang, and Chia-Ching Chu#. 2023. Infection patterns of ‘Candidatus Liberibacter europaeus’ in Cacopsylla oluanpiensis, a psyllid pest of Pittosporum pentandrum. Journal of Invertebrate Pathology. 200:107959. (SCI)
6. Wen-Yu Hsu*, Yi-Jin Lee*, Che-Hung Lin, and Chia-Ching Chu#. 2023. First report of Robbsia andropogonis causing bacterial leaf spot of bougainvilleas in Taiwan. Plant Disease. 107:(9)2835 (SCI)
7. Ching-Yu Chang*, Wen-Chien Tang*, and Chia-Ching Chu#. 2023. First report of Dickeya dadantii causing bacterial soft rot of Scindapsus pictus in Taiwan. Plant Disease. 107(8):2516 (SCI)
8. Nian-Pu Li*, Wen-Qian Tang*, Shin Lee, Chih-Li Wang, and Chia-Ching Chu#. 2023. First report of Pectobacterium carotovorum and P. brasiliense causing bacterial soft rot of bok choy in Taiwan. Plant Disease. 107(7):2216 (SCI)
9. Liang-Hsuan Wang*, Jiun-Jie Chan*, Yi-Hsin Wang, Zi-Qing Fang, Shin Lee and Chia-Ching Chu#. 2023. Bacterial leaf blight of Polyscias guilfoylei caused by a novel pathovar of Xanthomonas euvesicatoria. Plant Disease 107(2):298–305. (SCI)
10. Yu-Min Wu*, Liang-Hsuan Wang* and Chia-Ching Chu#. 2023. First report of Dickeya dadantii causing bacterial soft rot of Thaumatophyllum bipinnatifidum in Taiwan. Plant Disease 107(2):552. (SCI)
11. Liang-Hsuan Wang*, Wen-Qian Tang*, Jiun-Jie Chan, Yi-Jin Lee, Ching-Yu Chang, Zi-Qing Fang and Chia-Ching Chu#. 2023. First report of Pectobacterium aroidearum causing bacterial soft rot of Epipremnum aureum in Taiwan. Plant Disease 107(2):550. (SCI)
12. Shin Lee, Chien-Young Chu, and Chia-Ching Chu#. 2022. Expression level of a phenylalanine ammonia-lyase gene in poinsettia is negatively correlated with poinsettia branch-inducing phytoplasma titer. Microbiology Spectrum 10(6):e03814-22. (SCI)
13. Fang-Yu Lin, Shin Lee, Yi-Chang Liao, Man-Miao Yang, and Chia-Ching Chu#. 2022. Infection patterns of a Liberibacter associated with Macrohomotoma gladiata, a psyllid feeding on Ficus microcarpa. Microbiology Spectrum 10(6):e03614-22. (SCI)
14. Xian-Yong Wei, Wen-Ling Deng, and Chia-Ching Chu#. 2021. Phylogenetic and phenotypic analyses on Dickeya spp. isolated from different host plants in Taiwan. Journal of Phytopathology 169(11-12):678-691.(SCI)
15. Shin Lee*, Yi-Jin Lee*, Ching-Yu Chang*, and Chia-Ching Chu#. 2021. First report of a ‘Candidatus Phytoplasma aurantifolia’-related strain (16SrII-V) associated with phyllody, virescence, and shoot proliferation of sweet William (Dianthus barbatus) in Taiwan. Plant Disease 105(10):3285. (SCI)
16. Shin Lee, Chien-Young Chu, and Chia-Ching Chu#. 2021. Variability of phytoplasma infection density in poinsettia and evaluation of its association with the level of branching in host plants. Plant Disease 105 (5):1539-1545. (SCI)
17. Wen-Qian Tang, Ching-Yu Chang, Yi-Jin Lee, and Chia-Ching Chu#. 2021. First report of Pectobacterium aroidearum causing bacterial soft rot of carrot in Taiwan. Plant Disease 105 (3):695. (SCI).
18. Ying-Ru Lin, Shin Lee, Chih-Hung Lu, and Chia-Ching Chu#. 2020. Genetic and phenotypic characterization of Xanthomonas axonopodis pv. maculifoliigardeniae causing bacterial leaf spot of Ixora in Taiwan. Journal of Phytopathology 168(7-8):478-489. (SCI; Issue cover) 
19. Fang-Yu Lin*, Zi-Qing Fang*, Shin Lee, Yi-Hsin Wang, Ying-Ru Lin, and Chia-Ching Chu#. 2019. First report of Xanthomonas axonopodis pv. begoniae causing bacterial leaf spot on Rieger begonias in Taiwan. Plant Disease 103(11):2940. (SCI)
20. Chih-Hung Lu, Yen-Hsin Chiu, Jen-Yu Tzeng, Chia-Ying Lin, Ying-Ru Lin, Wen-Ling Deng and Chia-Ching Chu#. 2019. First report of Xanthomonas hortorum pv. hederae causing bacterial leaf spot of Hedera helix in Taiwan. Plant Disease 103(7):1765. (SCI)
21. Chia-Ching Chu, Mark Hoffmann, Warren E. Braswell, and Kirsten S. Pelz-Stelinski#. 2019. Genetic variation and potential co-infection of Wolbachia among widespread Asian citrus psyllid (Diaphorina citri Kuwayama) populations. Insect Science 26(4):671-682 (SCI)
22. Ying-Ru Lin, Shin Lee, Chih-Hung Lu, and Chia-Ching Chu#. 2019. First report of bacterial leaf spot of crown of thorns (Euphorbia milii) caused by Xanthomonas axonopodis pv. poinsetticola in Taiwan. Plant Disease 103(4):759. (SCI)
23. Xian-Yong Wei, Yen-Hsin Chiu, Wen-Ling Deng, and Chia-Ching Chu#. 2019. First report of Dickeya dadantii causing stem rot of poinsettia in Taiwan. Plant Disease 103(1):143. (SCI)
24. Chia-Ching Chu and Sydney A. Cameron#. 2017. A scientific note on Nosema bombi infection intensity among different castes within a Bombus auricomus nest. Apidologie 48:141-143. (SCI)
25. Torrence A Gill, Chia-Ching Chu, and Kirsten S. Pelz-Stelinkski#. 2017. Comparative proteomic analysis of hemolymph from uninfected and Candidatus Liberibacter asiaticus-infected Diaphorina citri. Amino Acids 49:389-406. (SCI)
26. Chia-Ching Chu, Torrence A. Gill, Mark Hoffmann, and Kirsten S. Pelz-Stelinski#. 2016. Inter-population variability of endosymbiont densities in the Asian citrus psyllid (Diaphorina citri Kuwayama). Microbial Ecology 71:999-1007. (SCI)
27. Charles-Antoine E. Dean, Michelle A. Duennes, Chia-Ching Chu, and Sydney A. Cameron#. 2015. Colony characteristics and Nosema infection of Bombus auricomus (Hymenoptera: Apidae). Entomological News 125:220-223. (SCI)
28. Chia-Ching Chu, Jorge A. Zavala, Joseph L. Spencer, Matías J. Curzi, Christopher J. Fields, Jenny Drnevich, Blair D. Siegfried, and Manfredo J. Seufferheld#. 2015. Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts. Evolutionary Applications 8:692-704. (SCI; Issue cover)
29. Haw-Chuan Lim#, Chia-Ching Chu, Manfredo J. Seufferheld#, and Sydney A. Cameron#. 2015. Deep sequencing and ecological characterization of the gut microbial communities of diverse bumble bee species, PLoS One DOI:10.1371/journal.pone.0118566 (SCI)
30. Chia-Ching Chu, Weilin Sun, Joseph L. Spencer, Barry R. Pittendrigh, and Manfredo J. Seufferheld#. 2014. Differential effects of RNAi treatments on field populations of the western corn rootworm, Pesticide Biochemistry and Physiology 110:1-6. (SCI; Issue cover)
31. Chia-Ching Chu, Joseph. L. Spencer, Matías J. Curzi, Jorge A. Zavala, and Manfredo J. Seufferheld#. 2013. Gut bacteria facilitate adaptation to crop rotation in the western corn rootworm, Proceedings of the National Academy of Sciences of the United States of America 110:11917-11922. (SCI; Issue cover)

    *equal contribution; #corresponding Author

2023        111 學年度農資學院優良導師

2021        109 學年度農資學院優良導師

2021        孫守恭教授植物病理學術獎傑出年輕植物病理學者獎

2019        107 學年度農資學院優良導師

2017-19    科技部補助大專校院延攬特殊優秀人才獎勵

2014        Fred Clute Memorial Award

2013        William H. Luckmann Award for Student Research in Applied Entomology

2011        Fred Clute Memorial Award

2010-12    J. C. Hackleman Fellowship/Illinois Crop Improvement Association Graduate Fellowship

2010        Fellowship of the Illinois Chapter of the Gamma Sigma Delta Agriculture Honors Society

2008        高等考試三級植物病蟲害防治類科合格

2007        Taiwan Honorary member of the Phi Tau Phi Scholastic Honor Society

2002        Ching O Medal, National Chung Hsing University

1. Wang, L.-H., Tang, W.-C., and Chu, C.-C.#. Genetic and phenotypic diversity of bacteria causing potato soft rot in Taiwan. International Conference of Clinical Plant Science 2023. Jeju Island, Korea. 2023/12/1-3. (Poster)
2. Y.-J. Lee, S. Lee, C.-Y. Chu, and Chu, C.-C.#. Investigating the effects of phytohormone treatment on the association between poinsettia and phytoplasma. International Conference of Clinical Plant Science 2023. Jeju Island, Korea. 2023/12/1-3. (Poster)
3. C.-Y. Chang, and Chu, C.-C.#. Screening of potato tuber-isolated streptomycetes for plant growth promotion and disease control. International Conference of Clinical Plant Science 2023. Jeju Island, Korea. 2023/12/1-3. (Poster)
4. Tang, W.-C., Wang, L.-H., and Chu, C.-C.#. Phylogenetic placements and characteristics of Pectobacterium strains obtained from different hosts in Taiwan. Plant Health 2023 (the APS Annual Meeting), Denver, CO, USA. 2023/08/12–16. (Poster)
5. Fang, Z.-Q., Lee, S., Liao, Y.-C., Yang, M.-M., and Chu, C.-C.#. 2022. Investigation on the ecology of a liberibacter associated with Cacopsylla oluanpiensis. The 2022 Annual Meeting of The Plant Protection Society of Republic of China. 2022/12/02.
6. Tang, W.-C., Wang. L.-H., Chu, C.-C.#. 2022. Phylogenetic diversity and maceration potentials of Pectobacterium species infecting different plant hosts in Taiwan. The 16th International Symposium on Biocatalysis and Agricultural Biotechnology, Taichung, Taiwan. 2022/11/9-11. (Poster)
7. Lin, F.-Y, Y.-C. Liao, M.-M. Yang, S. Lee, Chu, C.-C.#. 2022. Investigation on the ecology of a liberibacter in Macrohomotoma gladiata. The 2022 Annual Meeting of Taiwan Phytopathological Society, Taipei, Taiwan. 2022/4/30. (Presented online)
8. Lin, F.-Y., Chu, C.-C.#. 2021. Ecological characterization of a liberibacter detected in Macrohomotoma gladiata. 農業生技聯合研討會，南投縣，臺灣。2021/12/11。
9. Lee, S., Chu, C.-Y., Chu, C.-C.#. 2021. Infection pattern of poinsettia branch-inducing phytoplasma in its host plant. 農業生技聯合研討會，南投縣，臺灣。2021/12/11。
10. Lee, S., Chu, C.-Y., Chu, C.-C.#.2021. Investigation on the genetic diversity and infection patterns of poinsettia branch-inducing phytoplasma. The 2021 Annual Meeting of Taiwan Phytopathological Society, Taipei, Taiwan.
11. Wei, X.-Y., Deng, W.-L., Chu, C.-C.#. 2021. Genetic and phenotypic analyses on soft rot bacteria isolated from Phalaenopsis orchid in Taiwan. The 2021 Annual Meeting of Taiwan Phytopathological Society, Taipei, Taiwan.
12. Lu, C.-H., Wang, Y.-H., Su, S.-M., Chu, C. C.#.2021. Application of multiplex PMA-qPCR and PCR assays in detecting and quantifying xanthomonads causing bacterial leaf spot of tomatoes. The 2021 Annual Meeting of Taiwan Phytopathological Society, Taipei, Taiwan.
13. Wu, J. R., Wang, J. J., Chiu, Y. H., Wu, Y. F., Su, S. M., Chu, C. C.#. 2021. Investigation on the genetic and phenotypic diversity of phytopathogenic Streptomycetes in Taiwan. The 2021 Annual Meeting of Taiwan Phytopathological Society, Taipei, Taiwan.
14. 林映儒、李昕、盧志弘、朱家慶#。2019。感染台灣大戟科與茜草科花卉之 Xanthomonas 屬病原細菌之特性分析與檢測技術開發。中華民國植物病理學會 107 年度年會，台中市，台灣。
15. 魏憲雍、鄧文玲、朱家慶#。2019。台灣 Dickeya 屬軟腐細菌之系統發生分析與表型比較。中華民國植物病理學會 107 年度年會，台中市，台灣。
16. C.-C. Wang, Y.-J. Chen, T.-H. Liao, C.-Y. Kuo, Y.-L. Huang, C.-H. Wu, Y.-W. Tseng, Y.-H. Chang, S.-W. Chuang, C.-W. Chan, K.-H. Chen, H.-Y. Wang, J.-J. Tseng, C.-Y Gao, K.-T. Leung, C.-C. Chu# and M.-H. Lee#. Isolation of microbes and screening for their ability to degrade agriculture wastes and promote plant health. 9th International Conference of Clinical Plant Science. Dec. 1, 2018. Taichung, Taiwan (Poster).
17. X.-Y. Wei, W.-L. Deng, and C.-C. Chu#. Phylogenetic analyses of Dickeya spp. strains isolated from Taiwan. 9th International Conference of Clinical Plant Science. Dec. 1, 2018. Taichung, Taiwan (Poster).
18. Y.-R. Lin, S. Lee, C.-H. Lu and C.-C. Chu#. Identification and characterization of Xanthomonads causing bacterial leaf spots of ornamentals in Taiwan. 9th International Conference of Clinical Plant Science. Dec. 1, 2018. Taichung, Taiwan (Poster).
19. Y.-P. Tseng, C.-J. Han, and C.-C. Chu#. Investigation of the gut bacterial community in the lychee stinkbug (Tessaratoma papillosa). 9th International Conference of Clinical Plant Science. Dec. 1, 2018. Taichung, Taiwan (Poster).
20. Y.-H. Wang and C.-C. Chu#. Development of a viability qPCR workflow for detection of Xanthomonas euvesicatoria in tomato seeds. 9th International Conference of Clinical Plant Science. Dec. 1, 2018. Taichung, Taiwan (Poster).
21. Chu, C.-C#. Psyllid-microbe associations and their implications for management of plant diseases. 6th Taiwan−Japan Ecology Workshop. Nov 22-26, 2018. Tainan, Taiwan (Speaker).
22. Chu, C.-C#. Exploring the potential of using microbes as tools for managing insect-transmitted plant diseases. 2018 Trends in Sustainable Agriculture Conference. July, 5-6, 2018. Taipei, Taiwan, ROC. (Speaker)
23. 朱家慶#。植物病害媒介昆蟲與微生物交互作用之探討。2017年海峽兩岸植物病理學術研討會。2017年9月。湖北省，武漢市，中國。 (Speaker)
24. Chu, C.-C#. Insect-microbe interactions and their potential use in controlling insect-borne plant diseases. The 22nd Conference on Bacteriology. August, 2017. Taitung, Taiwan, ROC. (Speaker)
25. 朱家慶#。Investigating host-microbe associations in insect vectors of plant bacterial diseases. 2017年海峽兩岸植物保護博士後論壇暨青年學術研討會。2017年6月26日至27日，福建省，福州市，中國。(Speaker)
26. 朱家慶#。應用媒介昆蟲與微生物之交互作用防治蟲媒細菌性病害之可行性探討。中華民國植物病理學會105年度年會。2017年4月。台中市，台灣。 (Speaker)
27. Chu, C.-C., T. A. Gill,  M. Hoffmann, K. S. Pelz-Stelinski#. Ecological characterization of bacterial endosymbionts in field populations of the Asian citrus psyllid (Diaphorina citri Kuwayama). XXV International Congress of Entomology, Orlando, FL, USA, September, 25-30, 2016. (Speaker)
28. T. A. Gill, C.-C. Chu, K. S. Pelz-Stelinski#. Comparative analysis of hemolymph proteins derived from Diaphorina citri (ACP) either uninfected or infected with Candidatus Liberibacter asiaticus (CLas). The 4th Annual UF/IFAS CREC – Posters and Pastries Research Symposium, Lake Alfred, FL, USA, April, 8, 2016. (Poster)
29. Chu, C.-C., T. A. Gill, M. Hoffmann, K. S. Pelz-Stelinski#. Endosymbiont densities in Florida populations of the Asian citrus psyllid (Diaphorina citri Kuwayama). The 4th Annual UF/IFAS CREC – Posters and Pastries Research Symposium, Lake Alfred, FL, USA, April, 8, 2016. (Poster)
30. Chu, C.-C., T. A. Gill, K. S. Pelz-Stelinski#. Biological factors contributing to Candidatus Liberibacter asiaticus infection densities in field Asian citrus psyllid populations (Diaphorina citri Kuwayama). 63rd Annual ESA Meeting. Minneapolis, MN, USA, November, 14-18, 2015. (Poster)
31. Chu, C.-C., J. A. Zavala, J. L. Spencer, M. J. Curzi, C. J. Fields, J. Drnevich, B. D. Siegfried and M. J. Seufferheld#. Differential gene expression in rotation-resistant and wild-type western corn rootworm beetle (Diabrotica virgifera virgifera) digestive tracts. 63rd Annual ESA Meeting. Minneapolis, MN, USA, November, 14-18, 2015. (Poster)
32. Chu, C.-C., J. L. Spencer, M. J. Curzi, J. A. Zavala, and M. J. Seufferheld#. Host-microbe interactions in the rotation-resistant variant of the western corn rootworm, Diabrotica virgifera virgifera LeConte. 61st Annual ESA Meeting. Austin, TX, USA, November, 11-13, 2013. (Poster)
33. Chu, C.-C., M. J. Curzi, J. A. Zavala, J. L. Spencer, and M. J. Seufferheld#. Host-microbiota interactions and dietary adaptation of the rotation-resistant western corn rootworm (Diabrotica virgifera virgifera). 60th Annual ESA Meeting. Knoxville, TN, USA, November, 11-14, 2012. (Poster)
34. Chu, C.-C., M. J. Curzi, J. A. Zavala, J. L. Spencer, and M. J. Seufferheld#. Anthropogenic ecosystem disturbances facilitate the contemporary evolution of the western corn rootworm (Diabrotica virgifera virgifera). First Joint Congress on Evolutionary Biology. Ottawa, ON, Canada. July, 6-10, 2012. (Speaker)
35. M. J. Curzi, C.-C. Chu, J. L. Spencer, J. A. Zavala, and M. J. Seufferheld#. Effect of soybean herbivory on gut protease activity in wild-type and rotation-resistant WCR beetles. USDA Awardee Workshop. San Diego, CA, USA, December 11-12, 2010. (Poster)
36. P. F. Sung, Y. S. Leu, C.-C. Chu, S. T. Hsu, K. C. Tzeng#. 2007. Detection of Acidovorax avenae subsp. citrulli by SYBR Green real-time PCR. The 2007 Annual Meeting of Taiwan Phytopathological Society, Taichung City, Taiwan.
37. Chu, C.-C., K. C. Tzeng, W. L. Deng and S. T. Hsu#. 2006. Cloning and characterization of pecS/pecM from Erwinia chrysanthemi Sp13, a strain defective in blue pigment production. The 2006 Annual Meeting of Taiwan Phytopathological Society, Chia-Yi, Taiwan. (Speaker)

           # : Corresponding Author.

Manfredo J. Seufferheld, Chia-Ching Chu. 2019. Bacillus thuringiensis toxin resistance assay. U.S. Patent No. 10,415,078. (Issue date: Sep 17, 2019).

大學部

普通微生物學 (共同授課)、植物細菌學、普通微生物學實驗、專題研究 (上學期)、植物檢疫 (共同授課)

研究所

專題討論 (特定學期)、植物病害臨床診斷 (共同授課)、植物病理學特論 (共同授課)、植物病蟲害臨床診斷 (共同授課; 植醫學程)、植物病原細菌學文獻選讀

1. 王亮璇同學獲得科技部 111 年度大專生研究計畫補助。
2. 碩班生同學 林芳伃 於「中華民國植物病理學會 110 年度年會論文宣讀比賽」 B 組榮獲優選。2022/4/30。
3. 專任助理 李昕 於「農業生技聯合研討會英文壁報比賽」榮獲佳作。2021/12/11。
4. 唐文謙同學獲得 110 年度科技部大專生研究計畫補助。
5. 碩班生同學 李昕 於「中華民國植物病理學會 109 年度年會論文宣讀比賽」 B 組榮獲第三名。2021/5/1。
6. 碩班生同學 魏憲雍 於「中華民國植物病理學會 109 年度年會論文宣讀比賽」 B 組榮獲第二名。2021/5/1。
7. 曾宇霈同學獲得 108 年度科技部大專生研究計畫補助。
8. 大學部曾宇霈與韓喬融同學獲得第九屆國際植醫論壇海報競賽 Best Presentation Award (第三名)。
9. 魏憲雍同學獲得 107 年度科技部大專生研究計畫補助。

博士後研究員：

吳潤萍 博士

專任助理：

唐文謙

碩士班：

碩二 –  張靖俞、李宜瑾

碩一 –  王亮璇 (預備研究生)、詹竣傑、吳育旻、王程宏 (植醫學程)

大學部：

大三 –  張本萱、鄭依弦

大二 – 林冠頤

<其他詳細資訊>

碩士班

2019 畢 – 林映儒；2021 畢 – 李昕、盧志弘、吳家蓉、魏憲雍；2022 畢 – 林芳伃；2023 畢  – 方姿晴；2024 畢 – 唐文謙

Ecology of fastidious phytopathogenic bacteria

— 聖誕紅與聖誕紅分枝誘導性植物菌質體之交互作用

聖誕紅 (Euphorbia pulcherrima) 是世界上廣為栽培的盆花。幾乎市面上所有販售的聖誕紅皆具有良好的分枝性，而聖誕紅枝所以能產生這種性狀，主要是因為它們受到了聖誕紅分枝誘導性植物菌質體 (PoiBI) 的感染。除了分枝程度之外，PoiBI 也能改變聖誕紅植株葉片與苞片的顏色與形狀。也因此聖誕紅與 PoiBI 可說是植物病害中，極少數被廣為應用在園藝產業上的特殊案例。

一般聖誕紅育種者大多會先以雜交或是誘變的方式獲得遺傳背景不同的植株，並待植株長成後再植入 PoiBI，才能開始評估植株的性狀並進行品種檢定，其過程相當耗時。本團隊透過分子定量技術搭配扦插繁殖試驗發現，PoiBI 在不同植株間以及不同植株部位中的分佈情形相當不均勻。此外，本團隊也發現 PoiBI 在採穗母株 (stock plant) 中的族群量也與扦插繁殖苗呈現高分枝性的植株比例具正相關性 (Lee et al., 2021)。這些結果顯示，若能有效掌握 PoiBI 與寄主之交互作用，便能更有效率地改良聖誕紅性狀。

此外，本團隊近期也發現 PoiBI 在植株內的族群量與聖誕紅防禦相關基因之表現量呈現顯著的副相關性 (Lee et al., 2022)。未來本團隊將根據這些趨勢進行試驗設計，並針對 PoiBI 與聖誕紅之分子交互作用進行深入探討。

Publications:

1. Shin Lee, Chief-Young and Chia-Ching Chu#. 2022. Expression level of a phenylalanine ammonia-lyase gene in poinsettia is negatively correlated with poinsettia branch-inducing phytoplasma titer. Microbiology Spectrum 10(6): e03814-22. (SCI)
2. Shin Lee, Chien-Young Chu and Chia-Ching Chu#. 2021. Variability of phytoplasma infection density in poinsettia and evaluation of its association with the level of branching in host plants. Plant Disease 105 (5): 1539–1545. (SCI)

— Candidatus Liberibacter 屬細菌與媒介昆蟲之交互作用

Candidatus Liberibacter 能夠在一些重要作物上引起嚴重的病害 (如柑橘黃龍病；citrus greening)。這些病原細菌主要藉由歸屬於木蝨總科的昆蟲進行傳播。雖然近年來國際上有關 Ca. Liberibacter 的研究非常的多，但絕大多數的研究皆著重在少數的作物與媒介昆蟲種類。這樣的情形可能縮限了各界對 Ca. Liberibacter 與木蝨交互作用的認知。本團隊近期在棲息於臺灣木本植物中的木蝨發現了 Ca. Liberibacter 的存在 (Fang et al., 2023; Lin et al., 2022)。目前也發現有些菌種的在媒介昆蟲中的族群量可能會受到昆蟲性別、體色等因子的影響，而有些 Ca. Liberibacter 菌種的感染則可能與木蝨適性有關 。目前本團隊正在針對這個新的系統進行進一步的研究。

Publications:

1. Zi-Qing Fang, Yi-Chang Liao, Shin Lee, Man-Miao Yang, and Chia-Ching Chu#. 2023. Infection patterns of ‘Candidatus Liberibacter europaeus’ in Cacopsylla oluanpiensis, a psyllid pest of Pittosporum pentandrum. Journal of Invertebrate Pathology 200:107959. (SCI)
2. Fang-Yu Lin, Shin Lee, Yi-Chang Liao, Man-Miao Yang, and Chia-Ching Chu#. 2022. Infection patterns of a Liberibacter associated with Macrohomotoma gladiata, a psyllid feeding on Ficus microcarpa. Microbiology Spectrum 10(6): e03614-22. (SCI)

Genetic and phenotypic diversity of plant pathogenic bacteria

— Phylogenetic and phenotypic analyses on soft rot bacteria in Taiwan

屬於 Pectobacteriaceae 的各種軟腐細菌為相當常見的植物病原。過去臺灣分離到的軟腐細菌主要被區分為 Erwinia carotovora subsp. carotovora (Ecc) 與 Erwinia chrysanthemi (Ech)。隨著分子遺傳學的發展，目前國外已針對這些細菌的分類系統進行大幅的修改，而過去歸屬於 Ecc 與 Ech 的菌株目前已分別被歸類至 Pectobacterium 與 Dickeya 的不同物種當中。過去尚未有研究針對臺灣的菌株依照新的分類系統進行詳細的分析，也在病害診斷與植物檢疫方面衍生出了許多困擾。為了對臺灣軟腐細菌的分類地位與特性能有更進一步的認識，本團隊近年開始針對過去由臺灣田間分離到的菌株進行了系統發生學與表型分析。

本團隊近期已針對臺灣的 Dickeya 屬進行了相關的分析。試驗結果顯示臺灣至少可分離到 5 個不同的物種，而部分菌種似乎具有一定程度的寄主偏好性。強勢接種試驗發現，這些細菌的致腐能力在物種間與物種內皆具有高度變異性，而這些細菌在不同植物寄主中的致腐能力也有所不同 (Wei et al., 2021)。目前本團隊也開始針對臺灣分離之 Pectobacterium 菌株進行相同的分析 (Tang et al., 2024; Wang et al., in preparation)，期望這些試驗成果能幫助提升各界對臺灣軟腐細菌的生態與多樣性之了解，進而增進病害診斷與防治的精準度。

Publications:

1. Wen-Chien Tang, Liang-Hsuan Wang, Jiun-Jie Chan, Reun-Ping Goh, Yea-Fang Wu and Chia-Ching Chu#. 2024.  Inter- and intra-specific variations in phenotypic traits of Pectobacterium strains isolated from diverse eudicots and monocots in Taiwan. Plant Disease. (SCI; https://doi.org/10.1094/PDIS-10-23-2130-RE)
2. Xian-Yong Wei, Wen-Ling Deng, and Chia-Ching Chu#. Phylogenetic and phenotypic analyses on Dickeya spp. isolated from different host plants in Taiwan. Journal of Phytopathology 169 (11-12): 678-691. (SCI)

Discovery of novel bacterial plant pathogens

本研究室持續在田間搜尋過去未知之植物細菌。目前已成功發現與發表屬於 Xanthomonoas euvesicatoria 之全新病原型，全名為 Xanthomonoas euvesicatoria pv. polysciadis (Wang et al., 2023)。

Publications:

1. Liang-Hsuan Wang*, Jiun-Jie Chan*, Yi-Hsin Wang, Zi-Qing Fang, Shin Lee and Chia-Ching Chu#. 2023. Bacterial leaf blight of Polyscias guilfoylei caused by a novel pathovar of Xanthomonas euvesicatoria. Plant Disease 107(2): 298–305. (SCI)

Diagnosis of emerging bacterial diseases in Taiwan

本研究室致力於研究與鑑定臺灣新發現之植物細菌性病害。相關研究內容包括以生理生化測試搭配系統發生分析鑑定新發現之病原，或是進一步研發病原細菌之分子檢測方法，或是篩選有效的防治藥劑。本團隊近年已陸續在多種花卉與蔬菜作物報導多種由 Xanthomonas、Dickeya、Pectobacterium、Candidatus Phytoplasma、Robbsia 等細菌所引起的病害 (see publication list below)。

Publications:

1. Reun-Ping Goh*, Shin Lee*, Zi-Qing Fang*, Wen-Chien Tang* and Chia-Ching Chu#. 2024. First report of Pseudomonas cichorii causing bacterial leaf blight of pocketbook plant (Calceolaria hybrida) in Taiwan. Plant Disease. (SCI; Accepted) 
2. Reun-Ping Goh*, Shin Lee*, and Chia-Ching Chu#. 2024. First report of a ‘Candidatus Phytoplasma australasiaticum’-related phytoplasma strain associated with shoot proliferation disease of variegated croton in Taiwan. Plant Disease 108(3):781. (SCI)
3. Wen-Yu Hsu*, Yi-Jin Lee*, Che-Hung Lin, and Chia-Ching Chu#. 2023. First report of Robbsia andropogonis cau*sing bacterial leaf spot of bougainvilleas in Taiwan. Plant Disease 107:(9)2835. (SCI)
4. Ching-Yu Chang*, Wen-Chien Tang*, and Chia-Ching Chu#. 2023. First report of Dickeya dadantii causing bacterial soft rot of Scindapsus pictus in Taiwan. Plant Disease. 107(8):2516 (SCI)
5. Nian-Pu Li*, Wen-Qian Tang*, Shin Lee, Chih-Li Wang, and Chia-Ching Chu#. 2023. First report of Pectobacterium carotovorum and P. brasiliense causing bacterial soft rot of bok choy in Taiwan. Plant Disease. 107(7):2216 (SCI)
6. Yu-Min Wu, Liang-Hsuan Wang and Chia-Ching Chu#. 2023. First report of Dickeya dadantii causing bacterial soft rot of Thaumatophyllum bipinnatifidum in Taiwan. Plant Disease 107(2): 552. (SCI)
7. Liang-Hsuan Wang*, Wen-Qian Tang*, Jiun-Jie Chan, Yi-Jin Lee, Ching-Yu Chang, Zi-Qing Fang and Chia-Ching Chu#. 2023. First report of Pectobacterium aroidearum causing bacterial soft rot of Epipremnum aureum in Taiwan. Plant Disease 107(2):550. (SCI)
8. Shin Lee*, Yi-Jin Lee*, Ching-Yu Chang*, and Chia-Ching Chu#. 2021. First report of a ‘Candidatus Phytoplasma aurantifolia’-related strain (16SrII-V) associated with phyllody, virescence, and shoot proliferation of sweet William (Dianthus barbatus) in Taiwan. Plant Disease 105(10): 3285. (SCI)
9. Wen-Qian Tang, Ching-Yu Chang, Yi-Jin Lee, and Chia-Ching Chu#. 2021.  First report of Pectobacterium aroidearum causing bacterial soft rot of carrot in Taiwan. Plant Disease 105 (3), 695. (SCI)
10. Ying-Ru Lin, Shin Lee, Chih-Hung Lu, and Chia-Ching Chu#. 2020. Genetic and phenotypic characterization of Xanthomonas axonopodis pv. maculifoliigardeniae causing bacterial leaf spot of Ixora in Taiwan. Journal of Phytopathology 168(7-8): 478-489. (SCI; Issue cover)
11. Fang-Yu Lin*, Zi-Qing Fang*, Shin Lee, Yi-Hsin Wang, Ying-Ru Lin, and Chia-Ching Chu#. 2019. First report of Xanthomonas axonopodis pv. begoniae causing bacterial leaf spot on Rieger begonias in Taiwan. Plant Disease 103(11): 2940. (SCI)
12. Chih-Hung Lu, Yen-Hsin Chiu, Jen-Yu Tzeng, Chia-Ying Lin, Ying-Ru Lin, Wen-Ling Deng and Chia-Ching Chu#. 2019. First report of Xanthomonas hortorum pv. hederae causing bacterial leaf spot of Hedera helix in Taiwan. Plant Disease 103(7): 1765. (SCI)
13. Ying-Ru Lin, Shin Lee, Chih-Hung Lu, and Chia-Ching Chu#. 2019. First report of bacterial leaf spot of crown-of-thorns (Euphorbia milii) caused by Xanthomonas axonopodis pv. poinsettiicola in Taiwan. Plant Disease 103(4): 759. (SCI)
14. Xian-Yong Wei, Yen-Hsin Chiu, Wen-Ling Deng, and Chia-Ching Chu#. 2019. First report of Dickeya dadantii causing stem rot of poinsettia in Taiwan. Plant Disease 103(1): 143. (SCI).