{"id":6360,"date":"2025-02-02T15:02:24","date_gmt":"2025-02-02T07:02:24","guid":{"rendered":"https:\/\/www.pp.nchu.edu.tw\/?page_id=6360"},"modified":"2026-03-04T13:12:34","modified_gmt":"2026-03-04T05:12:34","slug":"%e6%a4%8d%e7%89%a9%e7%97%85%e6%af%92%e5%ad%b8%e7%a0%94%e7%a9%b6%e5%ae%a4%ef%bc%88laboratory-of-plant-virology","status":"publish","type":"page","link":"https:\/\/www.pp.nchu.edu.tw\/?page_id=6360","title":{"rendered":"\u690d\u7269\u75c5\u6bd2\u5b78\u7814\u7a76\u5ba4\uff08Laboratory of Plant Virology)"},"content":{"rendered":"
\"\"
\nChung-Hao Huang<\/span><\/div>\n
\n
Education and Experience<\/div>\n

National Chung Hsing University, Department of Plant Pathology\u00a0 Assistant professor\u00a0 2025.2-now
\nBio-ay Biotech Co., Ltd\u00a0 Research fellow\u00a0 2022.2-2025.1
\nTemasek Life Sciences Laboratory \u00a0\u00a0 Postdoctoral fellow\u00a0 2018.3-2021.12
\nNational Chung Hsing University, Department of Plant Pathology\u00a0 Postdoctoral fellow\u00a0 2017.2-2017.12
\nUniversity of California Davis, Department of Plant Pathology Postdoctoral fellow 2016.2-2017.1
\nDa-Yeh University\u00a0 Adjunct instructor 2014.8-2015.1
\nRockefeller University Visiting student 2012.3-2013.2
\nNational Chung Hsing University, Department of Plant Pathology\u00a0 PhD\u00a0 2007.9-2015.5
\nNational Dong Hwa University, Institute of Biotechnology\u00a0\u00a0Master\u00a0 2004.9-2007.1
\nNational Chung Hsing University, Department of Life Science\u00a0Bachelor\u00a0 \u00a02000.9-2004.6<\/p>\n

Contact information<\/div>\n

Tel: +886-4-22840780#303\u00a0
\nEmail:chunghao@nchu.edu.tw<\/a>
\nOffice: Agricultural and Environmental Science Building, 6D06<\/p>\n<\/div>\n

\n
Area of specification<\/div>
\n

Plant Virology<\/p>\n<\/div>\n

Research Interests<\/div>
\n

1. Development of plant virus vectors<\/p>\n

2. Plant virus\u2013host interactions<\/p>\n

3. Application of cross-protection by plant viruses<\/p>\n<\/div>\n

Teaching<\/div>
<\/span><\/p>\n

Plant virology, Plant virus-insect interactions, Transmission electron microscopy, Genetics<\/p>\n

<\/div><\/span><\/p>\n

Publications<\/div>
\n
    \n
  1. K. Chen#<\/sup>, P.J. Shih, H.Y. Chao, and C.H. Huang<\/u><\/strong> (2025). Characterization of zucchini tigre mosaic virus wax gourd isolate (ZTMV) and development of differentiating methods for identifying ZTMV and papaya ringspot virus (PRSV). J. Plant Med.<\/em> 67(4): 127-138. (#Corresponding author)<\/li>\n
  2. C. Chiu, C.H. Huang<\/u><\/strong>, and J.Y. Yang (2025) First Report of a 16SrII-A Subgroup \u2018Candidatus Phytoplasma Aurantifolia<\/em>\u2019-related Strain Infecting Pachyrhizus erosus<\/em> in Taiwan. New Disease Reports, 2025; 52:e70064<\/li>\n
  3. K Chen#<\/sup>, Y.S. Yang, and C.H. Huang<\/u><\/strong>. (2025) Characterization of a Colombian datura virus (CDV) isolated from Angel\u2019s trumpet (Brugmansia suaveolens<\/em>) J. Plant Med. <\/em>67 (2):61 (#Corresponding author)<\/li>\n
  4. S.D. Yeh, Y.C. Lin, C.C. Liao, C.C. Tseng, C.H. Huang<\/u><\/strong>, Y.L. Huang, S.L. Wang, and S.Y. Lin. (2024) Mapping the CP-Transgene Insert in the Papaya Genome and Developing a Hermaphrodite Transgenic Hybrid with Broad-Spectrum Resistance to Papaya Ringspot Virus. Viruses <\/em>16 (6), 823<\/li>\n
  5. \n

    W.C. Hu*, J.C Tsai*, H.W. Cheng, C.H. Huang, <\/u><\/strong>J.A. Raja, F.Y. Chang, C.C. Chen, C.H. Chiang, S.D. Yeh. (2024) The fifth residue of the coat protein of turnip mosaic virus is responsible for long-distance movement in a local-lesion host and aphid transmissibility in a systemic host. Phytopathology<\/em> 114 (7)<\/p>\n<\/li>\n

  6. \n

    \u00a0H.W. Cheng*, T.T. Lin*, C.H. Huang,<\/u><\/strong> J.A. Raja, S.D. Yeh. (2023) Modification of papaya ringspot virus HC-Pro to generate effective attenuated mutants for overcoming the problem of strain-specific cross protection. Plant Disease<\/em> 107(6):1757-1768<\/p>\n<\/li>\n

  7. \n

    S.H. Park*, J.S. Jeong*, C.H. Huang,<\/u><\/strong> B.S. Park, N.H. Chua. (2023) Inositol polyphosphates\u2010regulated polyubiquitination of PHR1 by NLA E3 ligase during phosphate starvation response in Arabidopsis. (New phytologist<\/em> 237(4):1215-1228)<\/p>\n<\/li>\n

  8. \n

    J.A. Raja*, C.H. Huang<\/u><\/strong>*,<\/strong><\/u> C.C Chen*, W.C. Hu, H.W Cheng, R.P. Wu, C.H. Chao, Y.R. Tan, S.D. Yeh. (2022) Modification of N-terminal FWKF-\u03b1H1element of potyviral HC-Pro affects its multiple functions and generates effective attenuated mutants for cross-protection. Molecular Plant Pathology<\/em> 23;7 947-65 (*equal contribution)<\/strong><\/p>\n<\/li>\n

  9. \n

    P.J. Chung*, G.P. Singh*, C.H. Huang<\/u><\/strong>*,<\/strong><\/u> S. Koyyappurath, J.S. Seo, H.Z. Mao, P. Diloknawarit, R. J. Ram, S. Rajani, N.H. Chua. (2021) Rapid detection and quantification of plant innate immunity response using Raman spectroscopy. Frontiers in Plant Science<\/em> 12, 746586 (*equal contribution)<\/strong><\/p>\n<\/li>\n

  10. \n

    J.A. Raja*, C.H. Huang<\/u><\/strong>*,<\/strong><\/u> C.C Chen*, W.C. Hu, H.W Cheng, R.P. Wu, C.H. Chao, Y.R. Tan, S.D. Yeh. (2022) Modification of N-terminal FWKF-\u03b1H1element of potyviral HC-Pro affects its multiple functions and generates effective attenuated mutants for cross-protection. Molecular Plant Pathology<\/em> 23;7 947-65 (*equal contribution)<\/strong><\/p>\n<\/li>\n

  11. \n

    \u00a0C.H. Huang<\/u><\/strong>*<\/u>, G.P. Singh*, S.H. Park, N.H. Chua, R.J. Ram, B.S. Park. (2020) Early diagnosis and management of nitrogen deficiency in plants utilizing Raman spectroscopy. Frontiers in Plant Science<\/em> 11, 663 (*equal contribution)<\/strong><\/p>\n<\/li>\n

  12. \n

    C.H. Huang<\/u><\/strong>, M.H. Foo, J.A.J. Raja, Y.R. Tan, T.T. Lin, S.S. Lin, S.D. Yeh. (2020) A Conserved Helix in the C-Terminal Region of Watermelon Silver Mottle Virus Nonstructural Protein S Is Imperative for Protein Stability Affecting Self-Interaction, RNA Silencing Suppression, and Pathogenicity. Molecular Plant-Microbe Interactions<\/em>. 33(4)637-52<\/p>\n<\/li>\n

  13. \n

    \u00a0T. Yao, B.S. Park, H.Z. Mao, J.S. Seo, N. Ohama, Y. Li, N. Yu, N.F.B Mustafa, C.H. Huang<\/u><\/strong>, N.H. Chua. (2019) Regulation of flowering time by SPL10\/MED25 module in Arabidopsis. New Phytologist<\/em>. 224 (1), 493-504<\/p>\n<\/li>\n

  14. \n

    B.S. Park, T. Yao, J.S. Seo, E.C.C. Wong, N. Mitsuda, C.H. Huang<\/u><\/strong>, N.H. Chua. 2018 Arabidopsis NITROGEN LIMITATION ADAPTATION regulates ORE1 homeostasis during senescence induced by nitrogen deficiency. Nature plants<\/em> 4(11), 898-903<\/p>\n<\/li>\n

  15. \n

    S. Poque, H.W. Wu, C.H. Huang<\/u><\/strong>, H.W. Cheng, W.C. Hu, J.Y. Yang, David Wang, S.D. Yeh. (2018) Potyviral gene silencing suppressor HCPro interacts with salicylic acid binding protein 3 to weaken SA mediated defense responses. Molecular Plant-Microbe Interactions<\/em>. 31(1)86-100<\/p>\n<\/li>\n

  16. \n

    J.S. Seo, H.X. Sun, B. Park, C.H. Huang<\/u><\/strong>, S.D. Yeh, C. Jung, N.H. Chua. (2017) Elf18-induced long noncoding RNA associates with Mediator to enhance expression of innate immune response genes in Arabidopsis. Plant Cell.<\/em> 29(5)1024-38<\/p>\n<\/li>\n

  17. \n

    C.H. Huang<\/u><\/strong>, W.R. Hsiao, C.W. Huang, K.C. Chen, S.S. Lin, T.C. Chen, J. A. Raja, H.W. Wu, S.D. Yeh. (2015) Two Novel Motifs of Watermelon Silver Mottle Virus NSs Protein Are Responsible for RNA Silencing Suppression and Pathogenicity. PLoS ONE<\/em> 10(5): e0126161.<\/p>\n<\/li>\n

  18. \n

    \u00a0Y. J. Kung, B. J. You, J. A. Raja, K. C. Chen, C. H. Huang, H. J. Bau, C. F. Yang, C.H. Huang<\/u><\/strong>, C. P. Chang, S. D. Yeh. (2015) Nucleotide sequence-homology-independent breakdown of transgenic resistance by more virulent virus strains and a potential solution. Scientific reports<\/em> 5:9804<\/p>\n<\/li>\n

  19. \n

    \u00a0K.N. Tsai, S.F. Tsang, C.H. Huang<\/u><\/strong>, and R.Y. Chang. (2007) Defective interfering RNAs of Japanese encephalitis virus found in mosquito cells and correlation with persistent infection. Virus Research<\/em> 124: 139-150.<\/p>\n<\/li>\n<\/ol>\n<\/div>\n

    Conference Abstracts<\/div>
    \n
      \n
    1. \n

      T.B. Tran, C.H. Huang<\/u><\/strong>, H.W. Cheng, C.C. Liao, J.A.J. Raja, S.D. Yeh (2025) Development of an effective bivalent vaccine for concurrent control of papaya ringspot virus and cucumber mosaic virus in cucurbits (Taichung, Taiwan, November 26-28, International Conference on Agricultural Biotechnology) (Oral presentation<\/em>)<\/p>\n<\/li>\n

    2. C.H. Huang<\/u><\/strong>, G.P. Singh, P.J Chung, J.S. Seo, S. Koyyppurath, R. Sarojam, R.J. Ram, B.S. Park, N.H. Chua (2025) Early diagnosis of abiotic and biotic stress in plant by Raman spectrometer (Taichung, Taiwan, April 26, The Annual meeting of Taiwan Phytopathology Society) (Oral presentation<\/em>)<\/li>\n
    3. \n

      S.D. Yeh, J. A. J. Raja, C.H. Huang<\/u><\/strong>, C.C. Chen, T.T.Y. Tran, S.Y. Lin, C.C. Liao. (2024) A novel method to generate mild virus vaccines by deletion mutation on the HC-Pro gene for control of potyviruses by cross protection (Bratislava, Slovak Republic October 16-18, International symposium on biocatalysis and agricultural biotechnology, ISBAB)<\/p>\n<\/li>\n

    4. \n

      C.H. Huang<\/u><\/strong>, G.P. Singh, N.H. Chua, R.J. Ram, B.S. Park. (2019) Early diagnosis of plant nutrient deficient response by Raman spectroscopy (Singapore, Augst.15-16, DiSTAP symposium)<\/p>\n<\/li>\n

    5. \n

      \u00a0Chang, R.Y., C.P. Yu, C.H. Huang<\/u><\/strong>, and C.-Y. Kan. The 9-nt (AACACGGAC) motif is a hot spot for template switch of reverse transcriptase between Japanese encephalitis viral genome and host 28S rRNA. (2016) Abstracts of 35th<\/sup>. American Society for Virology Annual Meeting (Blacksburg, Virginia, USA. June 18-22)<\/p>\n<\/li>\n

    6. \u00a0C.H. Huang<\/u><\/strong>, K.C. Chen, H.W.Wu, W.R. Hsiao, S.D.Yeh. (2011) Analysis of essential regions of NSs protein of Watermelon silver mottle virus <\/em>for gene silencing suppression.\u00a0 Abstracts of 15th<\/sup>. International Congress of Virology for IUMS 2011, (Sapporo, Sept. 11-15)<\/li>\n
    7. \n

      Y.C. Kang, W.T. Tsai, C.H. Huang<\/u><\/strong>, J.T. Li, J.S. Weng, S.D. Yeh, T.C. Chen. (2011) Prompt identification of Asian-type and EuroAmerican-type tospoviruses using monoclonal antibodies against the common epitopes of NSs proteins Abstracts of 15th<\/sup>. International Congress of Virology for IUMS 2011, (Sapporo, Sept. 11-15)<\/p>\n<\/li>\n

    8. \n

      \u00a0H.W. Wu, S.S. Lin, K.C. Chen, C.H. Huang<\/u><\/strong>, N.H. Chua, and S.D. Yeh. (2011) The roles of potyviral gene silencing suppressor on symptom severity and host defensive responses.\u00a0 Abstract of Annual meeting of Taiwan Phytopathology Society (Taichung, Mar. 25-26)<\/p>\n<\/li>\n

    9. \n

      \u00a0C.H. Huang<\/u><\/strong>, W.R. Hsiao, N.Y. Wang, C.W. Huang, S.D. Yeh. (2010) Analysis of essential regions of the NSs protein of Watermelon silver mottle virus<\/em> for pathogenicity by attenuated strain of Zucchini yellow mosaic virus<\/em> with point mutated in HC-Pro gene. Abstract of 12th<\/sup>.\u00a0 International Association Plant Biotechnology (IAPB) (St. Louis, June. 6-11)<\/p>\n<\/li>\n<\/ol>\n<\/div>\n<\/div>\n