{"id":1845,"date":"2017-12-03T02:52:57","date_gmt":"2017-12-02T18:52:57","guid":{"rendered":"http:\/\/www.pp.nchu.edu.tw\/?page_id=1845"},"modified":"2017-12-03T19:12:01","modified_gmt":"2017-12-03T11:12:01","slug":"%e5%88%86%e5%ad%90%e6%a4%8d%e7%89%a9%e7%97%85%e6%af%92%e7%a0%94%e7%a9%b6%e5%ae%a4-_%e8%8b%b1%e6%96%87%e7%89%88","status":"publish","type":"page","link":"https:\/\/www.pp.nchu.edu.tw\/?page_id=1845","title":{"rendered":"\u5206\u5b50\u690d\u7269\u75c5\u6bd2\u7814\u7a76\u5ba4 — _\u82f1\u6587\u7248"},"content":{"rendered":"

<\/a>\"\"<\/p>\n

\u8449\u932b\u6771 \u6559\u6388<\/span><\/p>\n<\/div>\n

\n
\u91cd\u8981\u5b78\u7d93\u6b77<\/div>\n

1983 \u7f8e\u570b\u5eb7\u5948\u723e\u5927\u5b78 (Cornell University) \u690d\u7269\u75c5\u7406\u5b78\u535a\u58eb
1979 \u4e2d\u8208\u5927\u5b78\u690d\u7269\u75c5\u7406\u5b78\u78a9\u58eb
1974 \u4e2d\u8208\u5927\u5b78\u690d\u7269\u75c5\u7406\u5b78\u5b78\u58eb<\/p>\n

2004\/08~2010\/12 \u570b\u7acb\u4e2d\u8208\u5927\u5b78\u526f\u6821\u9577
2006\/08\u8fc4\u4eca \u570b\u7acb\u4e2d\u8208\u5927\u5b78\u8b1b\u5ea7\u6559\u6388
1989\/08\u8fc4\u4eca \u570b\u7acb\u4e2d\u8208\u5927\u5b78\u690d\u7269\u75c5\u7406\u5b78\u7cfb\u6559\u6388
2001\/08~2004\/07 \u570b\u7acb\u4e2d\u8208\u5927\u5b78\u751f\u7269\u79d1\u6280\u767c\u5c55\u4e2d\u5fc3\u4e3b\u4efb
1984\/08~1989\/07 \u570b\u7acb\u4e2d\u8208\u5927\u5b78\u690d\u7269\u75c5\u7406\u5b78\u7cfb\u526f\u6559\u6388
1983\/11~1984\/07 \u7f8e\u570b\u5eb7\u5948\u723e\u5927\u5b78\u690d\u7269\u75c5\u7406\u5b78\u7cfb\u535a\u58eb\u5f8c\u7814\u7a76\u54e1
1976\/09~1979\/08 \u570b\u7acb\u4e2d\u8208\u5927\u5b78\u690d\u7269\u75c5\u7406\u5b78\u7cfb\u52a9\u7406\u7814\u7a76\u54e1<\/p>\n

\u806f\u7d61\u8cc7\u8a0a<\/div>\n

\u96fb\u8a71\uff1a04-22840780 #306 #322 #357
\u4fe1\u7bb1\uff1asdyeh@dragon.nchu.edu.tw
\u8fa6\u516c\u5ba4\uff1a\u8fb2\u74b0\u5927\u6a135D06
\u6559\u5e2b\u5c08\u9801\uff1a\u5efa\u7f6e\u4e2d<\/p>\n<\/div>\n

\u7814\u7a76\u65b9\u5411\u8207\u672a\u4f86\u767c\u5c55<\/div>
\n
    \n
  1. \u690d\u7269\u75c5\u6bd2\u5206\u5b50\u751f\u7269\u5b78<\/li>\n
  2. \u75c5\u6bd2\u9798\u86cb\u767d\u8f49\u57fa\u56e0\u690d\u7269<\/li>\n
  3. \u8f15\u75c7\u75c5\u6bd2\u4ea4\u8b77\u4fdd\u8b77\u7cfb\u7d71<\/li>\n
  4. \u75c5\u6bd2\u8a3a\u65b7\u5de5\u5177\u4e4b\u88fd\u5099<\/li>\n<\/ol>\n<\/div>\n
    \u7814\u7a76\u9818\u57df<\/div>
    \n

    \u672c\u5be6\u9a57\u5ba4\u4e3b\u8981\u7814\u7a76\u65b9\u5411\u70ba\u690d\u7269\u75c5\u6bd2\u5206\u5b50\u751f\u7269\u5b78\u53ca\u4ee5\u907a\u50b3\u5de5\u7a0b\u9632\u6cbb\u690d\u7269\u75c5\u6bd2\u75c5\u5bb3\uff0c\u6240\u7814\u7a76\u7684\u690d\u7269\u75c5\u6bd2\u5305\u62ec\u869c\u87f2\u50b3\u64ad\u7684 Potyvirus \u5c6c\u4e2d\u7684\u6728\u74dc\u8f2a\u9ede\u75c5\u6bd2 (papaya ringspot virus<\/em>, PRSV)\u3001\u6728\u74dc\u8f2a\u9ede\u75c5\u6bd2\u897f\u74dc\u578b ( PRSV W type) \u8207\u77ee\u5357\u74dc\u9ec3\u5316\u5d4c\u7d0b\u75c5\u6bd2 (zucchini yellow mosaic virus<\/em>, ZYMV)\uff0c\u4ee5\u53ca\u858a\u99ac\u50b3\u64ad\u7684 Tospovirus \u5c6c\u4e2d\u7684\u897f\u74dc\u9280\u6591\u75c5\u6bd2 (watermelon silver mottle virus<\/em>, WSMV)\u3001\u82b1\u751f\u74b0\u6591\u75c5\u6bd2 (groundnut ringspot virus, GRSV)\u3001\u9cf3\u4ed9\u82b1\u75bd\u6591\u75c5\u6bd2 (impatiens necrotic spot virus<\/em>, INSV)\u3001\u82b1\u751f\u9ec3\u5316\u6247\u6591\u75c5\u6bd2 (peanut chlorotic fan spot virus<\/em>, PCFV) \u7b49\u3002
    \u5176\u4e3b\u8981\u4e4b\u75c5\u6bd2\u9632\u6cbb\u7b56\u7565\u8207\u7814\u7a76\u5de5\u4f5c\u5927\u81f4\u53ef\u5206\u70ba\u56db\u985e\uff1a<\/p>\n

      \n
    1. \u75c5\u6bd2\u57fa\u56e0\u9ad4\u6838\u9178\u5e8f\u5217\u89e3\u6790\u53ca\u57fa\u56e0\u529f\u80fd\u5206\u6790\uff1b<\/li>\n
    2. \u5c07\u75c5\u6bd2\u57fa\u56e0\u5c0e\u5165\u5bc4\u4e3b\u690d\u7269\u4ee5\u7522\u751f\u6297\u75c5\u6bd2\u6027\u72c0\uff1b<\/li>\n
    3. \u4ee5\u907a\u50b3\u5de5\u7a0b\u65b9\u6cd5\u69cb\u7bc9\u8f15\u75c7\u75c5\u6bd2\u7cfb\u7d71\u4f86\u9632\u6cbb\u5f37\u7cfb\u75c5\u6bd2\u7684\u5371\u5bb3\uff1b<\/li>\n
    4. \u75c5\u6bd2\u8a3a\u65b7\u5de5\u5177\u4e4b\u88fd\u5099\u5305\u62ec\u75c5\u6bd2\u4e4b\u55ae\u5143\u8207\u591a\u5143\u6297\u9ad4\u4e4b\u88fd\u5099\u53ca\u6838\u9178\u63a2\u91dd\u4e4b\u958b\u767c\u3002<\/li>\n<\/ol>\n<\/div>\n
      \u5df2\u5b8c\u6210\u7684\u76f8\u95dc\u7814\u7a76<\/div>
      \n
        \n
      1. \u5229\u7528\u8f15\u75c7\u75c5\u6bd2\u4e4b\u4ea4\u4e92\u4fdd\u8b77\u4f5c\u7528\u9632\u6cbb\u6728\u74dc\u8f2a\u9ede\u75c5\u6bd2<\/strong><\/span>
        \u6728\u74dc\u8f2a\u9ede\u75c5\u6bd2 (PRSV) \u70ba\u53f0\u7063\u53ca\u71b1\u5e36\u5730\u5340\u6728\u74dc\u683d\u57f9\u4e4b\u4e3b\u8981\u9650\u5236\u56e0\u5b50\uff0c\u8449\u6559\u6388\u5229\u7528\u4e9e\u785d\u9178\u7a81\u8b8a\u8655\u7406\u7372\u5f97\u7121\u75c5\u5fb5\u611f\u67d3\u4e4b\u8f15\u75c7\u75c5\u6bd2\uff0c\u5229\u7528\u4ea4\u4e92\u4fdd\u8b77\u4f5c\u7528 (cross protection) \u539f\u7406\u9632\u6cbbPRSV\uff0c\u6b64\u65b9\u6cd5\u7d93\u65bc1985-1994\u5e74\u9593\uff0c\u8655\u7406\u904e\u56db\u767e\u9918\u842c\u68f5\u6728\u74dc\u82d7\u6728\uff0c\u63a8\u5ee3\u9762\u7a4d\u9054\u4e8c\u5343\u9918\u516c\u9803\uff0c\u7dad\u6301\u53f0\u7063\u6728\u74dc\u4e4b\u751f\u7522\uff0c\u6b64\u70ba\u4e16\u754c\u4e0a\u5927\u898f\u6a21\u61c9\u7528\u4ea4\u4e92\u4fdd\u8b77\u4f5c\u7528\u9632\u6cbb\u869c\u87f2\u50b3\u64ad\u75c5\u6bd2\u4e4b\u9996\u4f8b\u3002\u6b64\u6210\u5c31\u65bc1988\u5e74\u7372\u7f8e\u570bPlant
        Disease\u671f\u520a\u9080\u8acb\u4f5c\u7279\u5225\u5831\u5c0e (Feature article)\uff0c\u4e26\u65bc1994\u5e74\u7372Advances in Disease Vector Research\u9080\u8acb\u505areview\u5831\u5c0e(Yeh et al., 1988; Yeh and Gonsalves, 1994)\u3002<\/li>\n
      2. \u6728\u74dc\u8f2a\u9ede\u75c5\u6bd2PRSV\u4e4b\u5206\u5b50\u7814\u7a76<\/strong><\/span>
        \u4e3b\u8981\u6210\u5c31\u5305\u62ec\u9996\u5148\u5c07\u6b64\u75c5\u6bd2\u4e4b\u5b8c\u6574\u57fa\u56e0\u6838\u9178\u9ad4\u5e8f\u521710326 nt\u89e3\u51fa\uff0c\u5efa\u69cb\u5176\u57fa\u56e0\u5716\u8b5c\uff0c\u70ba\u7576\u6642\u5df2\u77e5\u690d\u7269\u6b63\u6975\u6027ssRNA\u75c5\u6bd2\u5df2\u89e3\u5e8f\u8005\u4e4b\u6700\u9577\u8005 (Yeh et al., 1992)\u3002\u57fa\u65bc\u6b64\uff0c\u4ee5T3\u555f\u52d5\u5b50\u9a45\u52d5\u4e4b\u751f\u9ad4\u5916\u5177\u611f\u67d3\u529b\u8f49\u9304\u9ad4in vitro infectious transcript\u53ca\u4ee535S promoter\u555f\u52d5\u4e4b\u751f\u9ad4\u5167\u5177\u611f\u67d3\u529b\u8f49\u9304\u9ad4in vivo infectious transcript\u4ea6\u5df2\u69cb\u7bc9\u5b8c\u6210 (Chiang et al., 1997; Wang et al., 1997)\uff0c\u6b64\u4f7f\u5f97\u8449\u6559\u6388\u5be6\u9a57\u5ba4\u6709\u80fd\u529b\u5c0dPRSV\u7684\u4efb\u4f55\u57fa\u56e0\u505a\u529f\u80fd\u5206\u6790\u3002\u5229\u7528\u5f37\u7cfb\u75c5\u6bd2\u548c\u524d\u8ff0\u8f15\u75c7\u75c5\u6bd2\u5728cDNA\u5c64\u6b21\u4e0a\u4e4b\u91cd\u7d44\u5206\u6790\uff0c\u5df2\u627e\u51fa\u75c5\u539f\u6027\u76f8\u95dc\u4e4b\u5340\u57df\u3002\u76ee\u524d\u6b63\u5229\u7528\u9019\u4e9b\u57fa\u790e\uff0c\u69cb\u7bc9\u672c\u7701\u5206\u96e2\u682a\u4e0d\u5177\u75c5\u539f\u6027\u7684\u907a\u50b3\u5de5\u7a0b\u4e8c\u4ee3\u75ab\u82d7\u4f9b\u4ea4\u4e92\u4fdd\u8b77\u4f5c\u7528\u9632\u6cbbPRSV\u3002\u4e14\u5df2\u89e3\u51fa\u611f\u67d3\u6728\u74dc\u6700\u95dc\u9375\u4e4b\u56e0\u5b50\u70baNIa-Pro\u57fa\u56e0\u4e0a\u7684\u4e00\u500bLys27\u80fa\u57fa\u9178 (Chen et al., 2008)\uff0c\u8449\u6559\u6388\u5be6\u9a57\u5ba4\u65bc\u6728\u74dc\u8f2a\u9ede\u75c5\u6bd2\u4e4b\u5206\u5b50\u7814\u7a76\u5df2\u53d6\u5f97\u4e16\u754c\u5148\u5c0e\u5730\u4f4d\u3002<\/li>\n
      3. \u6297\u75c5\u6bd2\u4e4b\u57fa\u56e0\u5de5\u7a0b\u4f5c\u7269\u4e4b\u80b2\u6210<\/strong><\/span>
        \u7531PRSV\u75c5\u6bd2\u5168\u9577\u5ea6\u57fa\u56e0\u9ad4\u5b9a\u5e8f\u3001\u75c5\u6bd2\u6838\u9798\u86cb\u767d\u8f49\u57fa\u56e0\u7684\u69cb\u7bc9\u3001\u6728\u74dc\u57fa\u56e0\u8f49\u6b96\u8207\u7d44\u7e54\u57f9\u990a\u6280\u8853\u5e73\u53f0\u7684\u5efa\u7acb\uff0c\u4e00\u76f4\u5230\u5b8c\u6210\u7531\u653f\u5e9c\u76f8\u95dc\u55ae\u4f4d\u6240\u6307\u5b9a\u4e4b\u5404\u968e\u6bb5\u7530\u9593\u8a66\u9a57\uff0c\u767c\u5c55\u51fa\u4e00\u5957\u975e\u5e38\u6709\u6548\u7684\u6728\u74dc\u8f2a\u9ede\u75c5\u6bd2\u9632\u6cbb\u7b56\u7565\uff0c\u4e26\u4e14\u53c3\u8207\u5404\u9805\u75c5\u6bd2\u75c5\u5bb3\u9632\u6cbb\u53ca\u8f49\u57fa\u56e0\u6280\u8853\u7684\u570b\u969b\u6703\u8b70\uff0c\u6210\u70ba\u570b\u969b\u4e0a\u8457\u540d\u7684\u9632\u6cbb\u6728\u74dc\u8f2a\u9ede\u75c5\u6bd2\u7684\u5c08\u5bb6\u3002\u8449\u6559\u6388\u5be6\u9a57\u5ba4\u7368\u529b\u7814\u767c\u6728\u74dc\u8f49\u578b\u4f5c\u7528\uff0c\u7814\u767c\u51fa\u5e7c\u80da\u518d\u751f\u53ca\u9798\u86cb\u767d\u57fa\u56e0\u8fb2\u687f\u83cc\u8f49\u6b96\u6280\u8853 (Cheng et al., 1996)\uff0c\u76ee\u524d\u5df2\u6709\u591a\u500b\u6728\u74dc\u8f2a\u9ede\u75c5\u6bd2\u9798\u86cb\u767d\u8f49\u57fa\u56e0\u6728\u74dc\u682a\u7cfb\uff0c\u5177\u5099\u6975\u4f73\u6297PRSV\u4e4b\u6027\u72c0 (Fan et al., 2009; Kung et al., 2010)\uff0c\u5df2\u5b8c\u6210\u6eab\u5ba4\u8a55\u4f30\u3002\u4e14\u65bc1996\u5e74\u53d6\u5f97\u8fb2\u59d4\u6703\u7530\u9593\u8a66\u9a57\u8a31\u53ef\uff0c\u5df2\u5b8c\u6210\u8fb2\u8a66\u6240\u8f49\u57fa\u56e0\u5c08\u7528\u8a66\u9a57\u7530\u9032\u884c\u7530\u9593\u8a55\u4f30\uff0c\u7d50\u679c\u975e\u5e38\u986f\u8457\uff0c\u5c0d\u6728\u74dc\u7522\u696d\u6709\u9769\u547d\u6027\u885d\u64ca\u3002\u6240\u6709\u7530\u9593\u8a66\u9a57\u7d50\u679c\u5df2\u65bc2003\u5e74\u7372\u8fb2\u59d4\u6703\u751f\u7269\u5b89\u5168\u5be9\u67e5\u59d4\u54e1\u6703\u901a\u904e\uff0c\u6b64\u70ba\u6211\u570b\u7b2c\u4e00\u5b97\u8f49\u57fa\u56e0\u4f5c\u7269\u7530\u9593\u8a66\u9a57\u7bc4\u4f8b\u3002\u800c\u8449\u6559\u6388\u6240\u7814\u767c\u4e4b\u8f49\u57fa\u56e0\u6728\u74dc\u5c0d\u4e16\u754c\u5404\u5730\u4e4b\u6728\u74dc\u8f2a\u9ede\u75c5\u6bd2\u5747\u6709\u6297\u6027\uff0c\u5ee3\u53d7\u5404\u570b\u91cd\u8996\uff0c\u6b64\u65b9\u9762\u4e4b\u6210\u5c31\u7372\u9078\u70ba\u7f8e\u570b\u671f\u520aPhytopathology (Bau et al., 2003, Jan.) \u8207Plant Disease (Bau et al., 2004, June) \u4e4b\u5c01\u9762\u5831\u5c0e\u3002 \u53e6\u5916\u6e20\u7b49\u4ea6\u5df2\u5b8c\u6210CMV CP\u8f49\u57fa\u56e0\u83f8\u8349\u53ca\u756a\u8304\u3001TMV CP\u53careplicase\u8f49\u57fa\u56e0\u83f8\u8349\u53caPWV CP\u8f49\u57fa\u56e0\u83f8\u8349 (\u898b\u8457\u4f5c)\u3002\u76ee\u524d\u6b63\u9032\u884c\u6297Tospovirus\u3001ZYMV\u53caPRSV-W\u4e4b\u591a\u91cd\u6297\u75c5\u6027\u8f49\u57fa\u56e0\u751c\u74dc\u53ca\u897f\u74dc\u4e4b\u69cb\u7bc9\uff0c\u5df2\u6709\u826f\u597d\u7d50\u679c\u3002\u53e6\u5916\u65bc\u6728\u74dc\u8f2a\u9ede\u75c5\u6bd2\u9632\u6cbb\u8a66\u9a57\u7576\u4e2d\uff0c\u65bc\u7530\u9593\u767c\u73fe\u53e6\u4e00\u6728\u74dc\u5d0e\u8449\u5d4c\u7d0b\u75c5\u6bd2 (Papaya leaf distortion mosaic virus, PLDMV) \u53ef\u611f\u67d3\u6297PRSV\u4e4b\u8f49\u57fa\u56e0\u6728\u74dc (Bau et al., 2008)\uff0c\u7232\u89e3\u6c7a\u6b64\u554f\u984c\uff0c\u8a72\u7814\u7a76\u5ba4\u80b2\u6210\u4e86\u540c\u6642\u5177\u6709PRSV\u8207PLDMV\u4e4b\u9798\u86cb\u767d\u57fa\u56e0\u7684\u8f49\u57fa\u56e0\u6728\u74dc\uff0c\u4e26\u65bc\u9694\u96e2\u6eab\u5ba4\u63a5\u7a2e\u8a66\u9a57\u4e2d\u7372\u5f97\u53ef\u540c\u6642\u62b5\u6297PSRV\u8207PLDMV\u4e8c\u8005\u4e4b\u6297\u6027 (Kung et al., 2009; \u5341\u4e00\u6708\u5c01\u9762\u5831\u5c0e)\u3002\u76ee\u524d\u5df2\u7372\u5f97\u8fb2\u59d4\u6703\u7684\u8a31\u53ef\uff0c\u9032\u884c\u7530\u9593\u8a66\u9a57\u3002\u6b64\u5177\u5099\u96d9\u91cd\u6297\u75c5\u6bd2\u6027\u72c0\u6728\u74dc\u4e4b\u6210\u679c\uff0c\u5df2\u7a69\u5c45\u4e16\u754c\u9818\u5148\u4e4b\u4f4d\u7f6e\u3002<\/li>\n
      4. Tospovirus\u75c5\u6bd2\u5c6c\u4e4b\u7814\u7a76<\/strong><\/span>
        \u8fd1\u5e74\u4f86\u53f0\u7063\u897f\u74dc\u53ca\u5176\u4ed6\u74dc\u985e\u5ee3\u53d7\u7531\u858a\u99ac\u50b3\u64ad\u4e4b\u75c5\u6bd2\u5371\u5bb3\uff0c\u6b64\u75c5\u6bd2\u7d93\u5206\u96e2\u9451\u5b9a\u53ca\u8840\u6e05\u6bd4\u5c0d\uff0c\u8b49\u5be6\u548cTospovirus\u5c6c\u4e2d\u5df2\u77e5\u75c5\u6bd2\u4e0d\u540c\u3002\u8449\u6559\u6388\u9032\u4e00\u6b65\u5b8c\u6210\u6b64\u75c5\u6bd2\u4e4bnucleocapsid (N) gene\u4e4b\u5206\u6790\u6bd4\u5c0d\uff0c\u78ba\u8a8d\u6b64\u70baTospovirus\u4e4b\u65b0\u75c5\u6bd2\uff0c\u4e26\u547d\u70ba\u897f\u74dc\u9280\u6591\u75c5\u6bd2 (Watermelon silver mottle virus, WSMoV) (Yeh and Chang, 1995)\u3002\u6b64\u75c5\u6bd2\u4e4b\u5168\u9577\u5ea6S RNA (Yeh et al., 1996) \u53caM RNA (Chu and Yeh, 1998) \u4e4b\u5168\u9577\u5e8f\u5217\u5206\u6790\u5747\u5df2\u5b8c\u6210\uff0c\u5176N-gene\u8f49\u57fa\u56e0\u83f8\u8349\u53ca\u756a\u8304\u4ea6\u5df2\u80b2\u6210\u3002\u9664WSMoV\u5916\uff0c\u8a72\u5be6\u9a57\u5ba4\u76ee\u524d\u5c1a\u6709\u843d\u82b1\u751f\u5206\u96e2\u682a\u53ca\u8774\u8776\u862d\u5206\u96e2\u682a\u5747\u5177\u8840\u6e05\u7279\u7570\u6027\u70ba\u4e8c\u53ef\u80fd\u65b0\u7a2e (Yeh et al., 1996; Chen et al., 2005)\u3002\u5be6\u9a57\u5ba4\u76ee\u524d\u4e26\u81ea\u884c\u88fd\u5099\u4e16\u754c\u4e0a\u6700\u9f4a\u5168\u4e4bserogroup I-VI\u4e4b\u5404\u7a2e\u55ae\u5143\u53ca\u591a\u5143\u6297\u9ad4\uff0c\u5df2\u6210\u70ba\u5168\u7403Tospovirus\u5c6c\u75c5\u6bd2\u9451\u5b9a\u53ca\u5206\u985e\u91cd\u93ae\u3002\u7531\u65bc\u5728Tospovirus\u5c6c\u4e0a\u4e4b\u512a\u7570\u6210\u5c31\uff0c\u8449\u6559\u6388\u65bc1995\u5e74\u7372\u9078\u70ba\u570b\u969b\u75c5\u6bd2Tospovirus study group\u570b\u969b\u4e03\u540d\u59d4\u54e1\u4e4b\u4e00\u6210\u54e1\uff0c\u70ba\u500b\u4eba\u53ca\u570b\u5bb6\u4e4b\u5149\u69ae\u3002\u5728\u570b\u969b\u4e0a\u5c0d\u65bc\u6b64\u5c6c\u75c5\u6bd2\u7684\u7814\u7a76\u5df2\u4f54\u6709\u9996\u5e2d\u4e4b\u5730\uff0c\u6e20\u4e26\u65bc2003\u5e74\u7372\u570b\u969b\u91cd\u8981\u66f8\u520aAdvances in Plant Disease Management\u4e4b\u9080\u64b0\u5beb\u5c08\u7ae0\u300cOccurrence, Importance, Taxonomy and Control of Thrips-borne Tospoviruses\u300d\uff0c\u53ef\u898b\u5176\u6210\u5c31\u975e\u51e1\u3002\u76ee\u524d\u5df2\u5c07WSMoV\u4e2dL RNA\u4e4b\u9ad8\u4fdd\u7559\u6027\u5e8f\u5217\u4ee5\u4e0d\u540c\u4e4b\u69cb\u7bc9\u5f62\u5f0f\u8f49\u6b96\u65bc\u83f8\u8349\uff0c\u53ef\u7372\u5f97\u5177\u6709\u5ee3\u6548\u6027\u5c0d\u6297\u5169\u7a2e\u4e0d\u540ctospoviruses\u7684\u8f49\u57fa\u56e0\u690d\u682a\uff0c\u4e14\u6297\u6027\u8868\u73fe\u826f\u597d\u3002\u83f8\u8349\u6a21\u5f0f\u5efa\u7acb\u5f8c\uff0c\u76ee\u524d\u6b63\u52aa\u529b\u80b2\u6210\u8f49\u57fa\u56e0\u756a\u8304\uff0c\u4ee5\u671f\u80fd\u5be6\u969b\u61c9\u7528\u65bc\u7d93\u6fdf\u4f5c\u7269\u4ee5\u9632\u6cbb\u71b1\u5e36\u53ca\u4e9e\u71b1\u5e36tospoviruses\u6240\u9020\u6210\u7684\u56b4\u91cd\u7d93\u6fdf\u640d\u5931\u3002\u8449\u6559\u6388\u5728tospoviruses\u7684\u5075\u6e2c\u6aa2\u75ab\u5de5\u4f5c\u4e0a\u6210\u679c\u8f1d\u714c\uff0c\u5982\u591a\u5143\u6216\u55ae\u682a\u6297\u9ad4\u4e4b\u88fd\u5099 (Chen et al., 2006\uff0c\u4e2d\u83ef\u6c11\u570b\u767c\u660e\u5c08\u5229\u7b2cI301853\u865f)\uff0c\u4ee5\u53ca\u5c08\u4e00\u6027\u6216\u7c21\u4f75\u6027\u5f15\u5b50\u5c0d\u4e4b\u958b\u767c\u7b49 (Chu et al., 2001a & b)\uff0c\u53ef\u91dd\u5c0dtospoviruses\u7684\u591a\u6578\u75c5\u6bd2\u6216\u662f\u500b\u5225\u8840\u6e05\u578b\u7684\u75c5\u6bd2\u9032\u884c\u5feb\u901f\u66a8\u6e96\u78ba\u4e4b\u5075\u6e2c\u53ca\u5206\u985e\u3002\u9019\u4e9b\u6210\u679c\u7686\u6709\u52a9\u65bc\u5c0d\u7530\u9593\u6216\u81ea\u570b\u5916\u9032\u53e3\u7684\u690d\u7269\u6aa2\u9ad4\u63d0\u4f9b\u826f\u597d\u7684\u9451\u5b9a\u4f9d\u64da\uff0c\u5728\u9632\u6aa2\u75ab\u53ca\u9632\u6cbb\u7684\u5de5\u4f5c\u4e0a\u7121\u7591\u662f\u4e0d\u53ef\u6216\u7f3a\u7684\u5229\u5668\u3002<\/li>\n
      5. \u690d\u7269\u75c5\u6bd2\u8f09\u9ad4\u4e4b\u958b\u767c\u5229\u7528<\/strong><\/span>
        \u8449\u6559\u6388\u4ea6\u5c0d\u690d\u7269\u75c5\u6bd2\u8f09\u9ad4\u4e4b\u5efa\u69cb\u6295\u5165\u4e0d\u5c11\u5fc3\u529b\uff0c\u5c07\u5206\u96e2\u81ea\u7530\u9593\u7f79\u75c5\u751c\u74dc\u4e4b\u77ee\u5357\u74dc\u9ec3\u5316\u5d4c\u7d0b\u75c5\u6bd2 (Zucchini yellow mosaic virus<\/em>, ZYMV) \u57fa\u56e0\u9ad4\u5b9a\u5e8f\u5f8c\uff0c\u958b\u767c\u6210\u75c5\u6bd2\u8f09\u9ad4\uff0c\u53ef\u65bc\u611f\u67d3\u5f8c\u5728\u690d\u7269\u9ad4\u5167\u5927\u91cf\u8868\u73fe\u6c34\u6bcd\u7da0\u8272\u87a2\u5149\u86cb\u767dGFP\u3001\u5875\u87a8\u904e\u654f\u539fDer p 5\uff0c\u4ee5\u53ca\u53e6\u4e00\u611f\u67d3\u74dc\u985e\u4e4b\u897f\u74dc\u9280\u6591\u75c5\u6bd2 (Watermelon silver mottle virus<\/em>, WSMoV) \u4e4b\u6838\u9798\u86cb\u767d (nucleocapsid protein, NP) \u8207\u975e\u7d50\u69cb\u6027NSs\u86cb\u767d\u3002\u6240\u8868\u73fe\u4e4b\u86cb\u767d\u7d93Ni2+-NTA\u89aa\u548c\u6027\u7ba1\u67f1\u7d14\u5316\u5f8c\uff0c\u53ef\u505a\u70ba\u6297\u8840\u6e05\u6216\u55ae\u682a\u6297\u9ad4\u88fd\u5099\u4e4b\u7528\u3002\u6b64\u5916\uff0c\u65bc\u52d5\u7269\u8a66\u9a57\u4e2d\u8b49\u5be6\u5c0f\u767d\u9f20\u9935\u98df\u542b\u6709ZYMV\u8868\u73fe\u4e4b\u5875\u87a8\u904e\u654f\u539fDer p 5\u7684\u690d\u7269\u6750\u6599\u5f8c\uff0c\u80fd\u6709\u6548\u5730\u964d\u4f4e\u904e\u654f\u53cd\u61c9\u7684\u6307\u6578\uff0c\u8a72\u6210\u679c\u4ea6\u65bc\u570b\u969b\u514d\u75ab\u5b78\u671f\u520a\u4e2d\u767c\u8868 (Hsu et al., 2004\uff0c\u4e2d\u83ef\u6c11\u570b\u767c\u660e\u5c08\u5229\u7b2cI288778\u865f)\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u4ee5\u5b9a\u9ede\u7a81\u8b8a\u65b9\u5f0f\u6240\u7522\u751f\u7684\u5f31\u7cfbZYMV\u8f09\u9ad4\u5247\u5177\u6709\u5728\u7cfb\u7d71\u6027\u5bc4\u4e3b\u4e0a\u50c5\u7522\u751f\u8f15\u5fae\u75c5\u5fb5\u4e14\u65bc\u55ae\u6591\u5bc4\u4e3b\u7121\u5c40\u90e8\u75c5\u6591\u5f62\u6210\u4e4b\u7279\u6027\uff0c\u76f8\u7576\u9069\u5408\u505a\u70ba\u7814\u7a76\u690d\u7269\u4ea4\u4e92\u4fdd\u8b77\u53ca\u75c5\u6bd2\u57fa\u56e0\u8207\u690d\u7269\u9593\u4ea4\u4e92\u4f5c\u7528\u95dc\u4fc2\u4e4b\u7528 (Lin et al., 2007; \u4e09\u6708\u5c01\u9762\u5831\u5c0e)\u3002\u6b64\u5916\uff0c\u4ee5\u958b\u767cZYMV\u75c5\u6bd2\u8f09\u9ad4\u4e4b\u7d93\u9a57\uff0c\u4ea6\u5c07\u5206\u96e2\u81ea\u5f69\u8272\u6d77\u828b\u4e4b\u856a\u83c1\u5d4c\u7d0b\u75c5\u6bd2 (Turnip mosaic virus, TuMV) \u5efa\u69cb\u6210\u70ba\u53e6\u4e00\u690d\u7269\u75c5\u6bd2\u8f09\u9ad4\uff0c\u4e26\u6210\u529f\u5730\u8868\u73feGFP\u3001Der p 5\u4ee5\u53caWSMoV\u4e4bNP\u8207NSs\u7b49\u86cb\u767d\uff0c\u7d93\u5b9a\u9ede\u7a81\u8b8a\u4e4b\u5f31\u7cfbTuMV\u4ea6\u80fd\u5c0d\u91ce\u751f\u578b\u75c5\u6bd2\u63d0\u4f9b\u826f\u597d\u7684\u4ea4\u4e92\u4fdd\u8b77\u6297\u6027 (Chen et al., 2007)\u3002<\/li>\n<\/ol>\n<\/div>\n
        \u6559\u6388\u8ab2\u7a0b<\/div>
        \n

        \u690d\u7269\u75c5\u6bd2\u5b78\u3001\u690d\u7269\u751f\u7269\u79d1\u6280\u5c08\u984c\u4ecb\u7d39\u3001\u8ad6\u6587\u5beb\u4f5c(\u4e00)\u3001\u5206\u5b50\u690d\u7269\u75c5\u6bd2\u5b78\u3001\u5206\u5b50\u75c5\u6bd2\u5b78\u6587\u737b\u9078\u8b80\u3001\u8ad6\u6587\u5beb\u4f5c\u3001\u690d\u75c5\u6587\u737b\u9078\u8b80<\/p>\n<\/div>\n

        \u671f\u520a\u8ad6\u6587<\/div>
        \n
          \n
        1. Yazhisai, U., Rajagopalan, P. A., Raja J. A. J., Chen, T.-C,
          and Yeh, S.-D.<\/strong> (2015) Untranslatable tospoviral NSs fragment
          coupled with L conserved region enhances transgenic resistance
          against the homologous virus and a serologically unrelated
          tospovirus. Transgenic Research. 24(4):635-649.<\/li>\n
        2. Kung, Y.-J., You, B.-J., Raja. J. A. J., Chen, K.-C., Huang,
          C.-H., Bau, H.-J., Yang, C.-F., Huang, C.-H., Chang, C.-P., and
          Yeh, S.-D.<\/strong> (2015) Nucleotide sequence-homology-independent
          breakdown of transgenic resistance by more virulent virus
          strains and a potential solution. Scientific Reports. 5:9804,
          1-10.<\/li>\n
        3. Huang, C.-H., Hsiao, W.-R., Huang, C.-W., Chen, K.-C., Lin,
          S.-S., Chen, T-C., Raja. J. A. J., Wu, H.-W., and Yeh, S.-D.<\/strong>
          (2015) Two novel motifs of Watermelon Silver Mottle Virus NSs
          protein are responsible for RNA silencing suppression and
          pathogenicity. PLoS One. 10:e0126161.<\/li>\n
        4. Chen, T.-C., Tsai, W.-T., Kang, Y.-C., Wang, Y.-C., Yeh,
          S.-D. (2015) Using monoclonal antibodies against the common
          epitopes of NSs proteins for the prompt detection and
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        5. Wu, P.-R., Chien, W.-C., Okuda, M., Takeshita, M., Yeh,
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        6. Lin, H.-T., Yen, G.-C., Lee, W.-C., Tsai, Y.-T. Wu, J.-H.,
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        7. Kung, Y.-J., Lin, S.-S., Huang, Y.-L., Chen, T.-C., Harish,
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          microRNAs targeting conserved motifs of the replicase gene
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        8. Chen, T.-C., Li, J.-T., Lin, Y.-P., Yeh, Y.-C., Kang, Y.-C.,
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          Calla lily chlorotic spot virus and design of broad-spectrum
          primers for detection of tospoviruses. Plant Pathology 61,
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        9. Yen, G.-C. Lin, H.-T., Cheng, Y.-H., Lin, Y.-J., Chang,
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        10. Lin, C.-Y., Ku, H.-M., Tan, C.-W., Yeh, S.-D.<\/strong> & Jan, F.-J.
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        11. Chen, T.-C., Lu, Y.-Y., Kang, Y.-C., Li, J.-T., Yeh, Y.-C.,
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          different tospoviruses species by the monoclonal antibody
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        12. Peng, J.-C., Yeh, S.-D.<\/strong>, Huang, L.-H., Li, J.-T., Cheng,
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          yellow spot virus on melon and watermelon in Taiwan. European
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        13. Lin, Y.-T., Lin, C.-W., Chung, C.-H., Su, M.-H., Ho, H.-Y.,
          Yeh, S.-D.<\/strong>, Jan, F.-J. & Ku, H.-M. (2011) In Vitro Regeneration
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        15. Kunkalikar, S. R., Poojari, S., Arun, B. M., Rajagopalan, P.
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        18. Wu, H.-W., Yu, T.-A., Raja, J. A. J., Christopher, S. J.,
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          transgenic oriental melon conferred by untranslatable chimeric
          construct carrying partial coat protein genes of two viruses.
          Plant Disease 94, 1341-1347.<\/li>\n
        19. Chen, Y.-T., Fang, Q.-S., Chiang, C.-H., Yeh, S.-D.<\/strong>, Wu,
          H.-W. & Yu, T.-A. (2010) Transgenic Eustoma grandiflorum
          expressing the bar gene are resistant to the herbicide Basta\u00ae.
          Plant Cell Tiss. Org. 102, 347-356.<\/li>\n
        20. Chao, C.-H., Wu, H.-W., Chen, K.-C., Lin, S.-S. & Yeh, S.-D.<\/strong>
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          of an attenuated mutant of Zucchini yellow mosaic virus. Plant
          Protection Bulletin 52, 1-16.<\/li>\n
        21. Chao, C.-H., Chen, T.-C., Kang, Y.-C, Li, J.-T., Huang,
          L.-H. & Yeh, S.-D.<\/strong> (2010) Characterization of Melon yellow spot
          virus infecting cucumber (Cucumis sativus L.) in Taiwan. Plant
          Pathology Bulletin 19, 41-52.<\/li>\n
        22. Kung, Y.-J., Yu, T.-A., Huang, C.-H., Wang, H.-C., Wang,
          S.-L. & Yeh, S.-D.<\/strong> (2010) Generation of hermaphrodite transgenic
          papaya lines with virus resistance via transformation of somatic
          embryos derived from adventitious roots of in vitro shoots.
          Transgenic Res. 19, 621-635.<\/li>\n
        23. Chen, T.-C., Lu, Y.-Y., Cheng, Y.-H., Li, J.-T., Yeh, Y.-C.,
          Kang, Y.-C., Chang, C.-P., Huang, L.-H., Peng, J.-C. & Yeh,
          S.-D. (2010) Serological relationship between Melon yellow spot
          virus and Watermelon silver mottle virus and differential
          detection of the two viruses in cucurbits. Arch. Virol. 155,
          1085-1095.<\/li>\n
        24. Wu, H.-W., Lin, S.-S., Chen, K.-C., Yeh, S.-D.<\/strong> & Chua, N.-H.
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          mosaic virus with differential effects on small RNA pathways
          involving viral pathogenicity and symptom development. Mol.
          Plant Microbe In. 23, 17-28.<\/li>\n
        25. Chen, C.-C., Huang, C.-H., Cheng, Y.-H., Chen, T.-C., Yeh,
          S.-D. & Chang, C.-A. (2009). First Report of Capsicum chlorosis
          virus Infecting Amaryllis and Blood Lily in Taiwan. Plant
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        26. Chang, H.-H., Tseng, H.-H., Yeh, S.-D.<\/strong>, Ku, H.-M. & Jan,
          F.-J. (2009). Generation of monoclonal antibody against the
          replicase of Watermelon silver mottle virus and its application
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        28. Kung, Y.-J., Bau, H.-J., Wu, Y.-L., Huang, C.-H., Chen,
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        29. Tsai, S.-F., Yeh, S.-D.<\/strong>, Chan, C.-F. & Liaw, S.-I. (2009).
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        30. Wu, H.-W., Yu, T.-A., Raja, J. A. J., Wang, H.-C. & Yeh,
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          to Zucchini yellow mosaic virus by an improved cotyledon-cutting
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        31. Lin, S.-S., Wu, H.-W., Elena, S.F., Chen, K.-C., Niu, Q.-W.,
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          threat of a new pathotype of Papaya leaf distortion mosaic virus
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        35. Zheng, Y.-X., Chen, C.-C., Yang, C.-J., Yeh, S.-D.<\/strong> & Jan,
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          Nucleotide sequence analysis of the coat protein genes of two
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        39. Wang, L.-Y., Chen, K.-C., Chen, T.-C. & Yeh, S.-D.<\/strong> (2007).
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            Yeh, S.-D.<\/strong>
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            Genetically Modified Papaya and the Influence on Soil Bacterial Communities in
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            Preparation of immunogens and production of antibodies. Methods Mol Biol. 508,
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            \u7372\u734e<\/div>
            \n

             <\/p>\n<\/div>\n

            \u7814\u8a0e\u6703\u8ad6\u6587<\/div>
            \n
              \n
            1. Lin, W. -Y, Chen, K. -C., Yang, C. -F., Uthaman Yazhisai,
              Yeh, S.-D.<\/strong> 2015. Generation of transgenic tomato lines with
              broad-spectrum resistance to tospoviruses and begomoviruses.
              Annual meeting of Taiwan Phytopathology Society. Abstract, SCB18
              (National Chung Hsing University, Taichung, Mar. 27-28, 2015)<\/li>\n
            2. Yeh, S.-D.<\/strong>, Chen, T. -C., Peng, J. -C., & Yazhisai Uthaman.
              2015. Diversity of Tospoviruses in Asia, and Possible Control by
              Broad-spectrum Transgenic Resistance. (Keynote Speech, Xth
              International Symposium on Thysanoptera and Tospoviurses,
              Monterey, California, USA, May. 16-20, 2015)<\/li>\n
            3. Chen, T. -C., Chien, W. -C., Tsai, W. -T., Kang, Y.-C.,
              Chen, Y.-H., Wang, Y. -C., Ou, Y. -J., & Yeh, S.-D.<\/strong> 2015. An
              efficient serological platform for identification and detection
              of tospoviral species prevalent in different geographic regions.
              (Oral Presentation, Xth International Symposium on Thysanoptera
              and Tospoviurses, Monterey, California, USA, May. 16-20, 2015)<\/li>\n
            4. Yeh, S.-D.<\/strong>, Kung, Y.-J., You, B.-J., Chen, K.-C., Bau,
              H.-J., Yang, J.-F., and Huang C.-H. 2015. Homology-independent
              breakdown of transgenic resistance by more virulent virus strain
              and the potential solution. (Keynote Speech, 11th Cross Strait
              Conference on Plant Molecular Biology and Plant Biotechnology,
              Wuhan, China, June 23-25, 2015)<\/li>\n
            5. Yeh, S.-D.<\/strong> 2015. Nucleotide Sequence-Homology-independent
              Breakdown of Transgenic Resistance by More Virulent Virus Strain
              and the Solution. (Keynote Speech, Guangxi University – Chung
              Hsing University on Agricultural and Biological Science
              Symposium, Guangxi, China, Oct. 27-31, 2015)<\/li>\n
            6. Yeh, S.-D.<\/strong>, Chang, C.-P., Chutirat Assawathep, Srimek
              Chowpongpang and Wichai Cositratana. 2015. Progress for control
              of Papaya ringspot virus inThailand by cross protection.
              (Keynote Speech, International Symposium for Agricultural
              Biotechnology, Thailand, Dec.16-20, 2015)<\/li>\n
            7. Kang, Y.-C., Chen, T.-C., Yeh, S.-D.<\/strong> 2015. Study on the gene
              silencing suppressor and pathogenicity determinant of Cucurbit
              chlorotic yellows virus. PV-07. (Kaohsiung District Agricultural
              Research and Extension Station, Pingtung, Taiwan, Dec. 25, 2015)<\/li>\n
            8. Chien, W.-C.\u201a Li, J.-T., Yang, C.-F., Yeh, S.-D.<\/strong> and Chen,
              T.-C. 2015. Generation of transgenic Nicotiana benthamiana
              plants conferring resistance against Cucurbit chlorotic yellows
              virus. PV-11. (Kaohsiung District Agricultural Research and
              Extension Station, Pingtung, Taiwan, Dec. 25, 2015)<\/li>\n
            9. Yeh, S.-D.<\/strong>, Lin, S.-S., and Chua, N.-H. 2007. A novel
              strategy for plant virus resistance using artificial miRNA. 2007
              International Symposium on Biocatalysis and Biotechnology,
              Abstract, p. 44 (National Chung Hsing University, Taichung,
              November 28-30, 2007)<\/li>\n
            10. Ho, H.-Y., Ku, H.-M., Yu, T.-A., Yeh, S.-D.<\/strong>, and Jan,
              Fuh-Jyh. 2007. Development of transgenic watermelon plants with
              multiple resistance to Cucumber mosaic-\u3001Cucumber green mottle
              mosaic- and Watermelon mosaic viruses . 2007 International
              Symposium on Biocatalysis and Biotechnology. (National Chung
              Hsing University, Taichung, November 28-30, 2007)<\/li>\n
            11. \u5f6d\u745e\u83ca\u3001\u912d\u5b89\u79c0\u3001\u8449\u932b\u6771<\/strong>\u30022008\u30022007\u5e74\u521d\u53f0\u5357\u5730\u5340\u6d0b\u9999\u74dc\u75c5\u6bd2\u75c5\u5bb3\u56b4\u91cd\u767c\u751f\u539f\u56e0\u4e4b\u63a2\u8a0e\u3002Annual meeting
              of Taiwan Phytopathology Society, Abstract, V-6 (National Chung
              Hsing University, Taichung, Jan. 25, 2008)<\/li>\n
            12. \u9673\u91d1\u679d\u3001\u6c5f\u82ac\u862d\u3001\u9673\u5b97\u797a\u3001\u8449\u932b\u6771<\/strong>\u3001\u9ec3\u6625\u60e0\u3001\u912d\u6afb\u6167\u3001\u5f35\u6e05\u5b89\u30022008\u3002Capsicum chlorosis
              virus\u611f\u67d3\u5b64\u633a\u82b1\u4e4b\u8840\u6e05\u5b78\u53ca\u5206\u5b50\u751f\u7269\u7279\u6027\u4e4b\u9451\u5b9a\u3002Annual meeting of Taiwan Phytopathology
              Society, Abstract, V-10 (National Chung Hsing University,
              Taichung, Jan. 25, 2008)<\/li>\n
            13. \u9673\u91d1\u679d\u3001\u9ec3\u6625\u60e0\u3001\u9673\u5b97\u797a\u3001\u8449\u932b\u6771<\/strong>\u3001\u912d\u6afb\u6167\u3001\u5f35\u6e05\u5b89\u30022008\u3002WsMoV\u611f\u67d3\u6d77\u828b\u4e4b\u8840\u6e05\u5b78\u53ca\u5206\u5b50\u751f\u7269\u7279\u6027\u4e4b\u9451\u5b9a\u3002Annual
              meeting of Taiwan Phytopathology Society, Abstract, V-11
              (National Chung Hsing University, Taichung, Jan. 25, 2008)<\/li>\n
            14. \u5f6d\u745e\u83ca\u3001\u9673\u7d39\u5d07\u3001\u8449\u932b\u6771<\/strong>\u30022008\u3002\u756a\u8304\u7a2e\u5b50\u7a2e\u82d7\u5e36\u75c5\u6bd2\u7387\u6aa2\u6e2c\u53ca\u767c\u5c55\u756a\u8304\u7a2e\u5b50\u53bb\u75c5\u6bd2\u6280\u8853\u4e4b\u7814\u7a76\u3002Annual meeting
              of Taiwan Phytopathology Society, Abstract, V-16 (National Chung
              Hsing University, Taichung, Jan. 25, 2008)<\/li>\n
            15. Dmytro, U., Chen, K. C. and Yeh, S. D.<\/strong> 2008. Construction of
              artificial miRNA for generating transgenic resistance against
              potyviruses in plant. Annual meeting of Taiwan Phytopathology
              Society, Abstract, SC-02 (National Chung Hsing University,
              Taichung, Jan. 25, 2008)<\/li>\n
            16. Wang, P. T., Chang, Y. L., Chiang, C. H., Chen, Y. T., Shaw,
              S. F., Yeh, S. D.<\/strong>, Shih, H. D. and Yu, T. A. 2008. Transgenic
              melons expressing antifungal protein (AFP3) conferred resistance
              against Rhizoctonia solani. Annual meeting of Taiwan
              Phytopathology Society, Abstract, SC-03 (National Chung Hsing
              University, Taichung, Jan. 25, 2008)<\/li>\n
            17. Chen, J. H., Li, C. M., Chiang, C. H., Yeh, S. D.<\/strong> and Yu, T.
              A. 2008. Double resistance of transgenic watermelon expressing
              an untranslatable chimeric construct carrying parts of coat
              protein coding sequence of Zucchini yellow mosaic virus and
              Papaya ringspot virus type-W. Annual meeting of Taiwan
              Phytopathology Society, Abstract, SC-04 (National Chung Hsing
              University, Taichung, Jan. 25, 2008)<\/li>\n
            18. Wu, H. W., Lin, S. S., Chen, K. C., Yeh, S. D.<\/strong>, Chua, N. H.
              2008. Discriminating mutations of HC-Pro with differential
              effects on miRNA and siRNA pathwas. International Union of
              Microbiological Societies 2008-XIV International Congress of
              Virology, Abstract, VOP-37, p. 28 (Istanbul, Turkish, Aug.
              10-15, 2008)<\/li>\n
            19. Chen, C. C., Yeh, S. D.<\/strong> 2008. Expression of dust mite
              allergen in edible leaves of crucifers by Turnip mosaic virus
              vector. International Union of Microbiological Societies
              2008-XIV International Congress of Virology, Abstract, VOP-229,
              p.142 (Istanbul, Turkish, Aug. 10-15, 2008)<\/li>\n
            20. Chen, K. C., Chiang, C. H., RAJA, J. A. J., Liu, F. L., Tai,
              C. H., Yeh, S. D.<\/strong> 2008. A signal amino acid of NIaPro of papaya
              ringspot virus determining host specificity for infection of
              papaya. International Union of Microbiological Societies
              2008-XIV International Congress of Virology, Abstract, VP-316,
              p.336 (Istanbul, Turkish, Aug. 10-15, 2008)<\/li>\n
            21. Chen, C. C., Chiang, F. L., Chen, T. C., Yeh, S. D.<\/strong>, Cheng,
              Y. H., and Chang, C. A. 2009. Serological and molecular
              identification of Capsicum chlorosis virus infecting blood lily.
              Annual meeting of Taiwan Phytopathology Society, Abstract, V04
              (National Chung Hsing University, Taichung, Feb. 6, 2009)<\/li>\n
            22. Yeh, Y. C., Lin, Y. P., Yeh, S. D.<\/strong>, Chen, T. C. 2009. Quick
              detection of tospoviruses using degenerate primers. Annual
              meeting of Taiwan Phytopathology Society, Abstract, SC25
              (National Chung Hsing University, Taichung, Feb. 6, 2009)<\/li>\n
            23. Kang, Y. C., Yeh, S. D.<\/strong>, Chen, T. C. 2009. Production of
              broad-spectrum antisera for detection of tospoviruses. Annual
              meeting of Taiwan Phytopathology Society, Abstract, SC27
              (National Chung Hsing University, Taichung, Feb. 6, 2009)<\/li>\n
            24. Li, J. X., Cheng, H. W., Yeh, S. D.<\/strong> 2009. Enhancement of the
              production of recombinant proteins expressed by Zucchini yellow
              mosaic virus vector through fusion with the nucleocapsid protein
              of a tospovirus. Annual meeting of Taiwan Phytopathology
              Society, Abstract, SC30 (National Chung Hsing University,
              Taichung, Feb. 6, 2009)<\/li>\n
            25. Yang, J. F., Yeh, S. D.<\/strong>, Chiang, C. H., Yu, T. A. 2009.
              Transgenic watermelon lines with triple-virus resistance
              generated by Agrobacterium-mediated transformation. Annual
              meeting of Taiwan Phytopathology Society, Abstract, SC33
              (National Chung Hsing University, Taichung, Feb. 6, 2009)<\/li>\n
            26. Kung, Y.-J., Lin, S.-S., Huang, Y.-L., Chua, N.-H. and Yeh,
              S.-D. 2009. Artificial microRNAs targeting the conserved motifs
              of replicase gene conferring transgenic resistance to
              negative-sense ssRNA plant virus. International Symposium on
              Vascular Biology and Agriculture (Chungqing, China, Jun. 21-24,
              2009)<\/li>\n
            27. Yeh, S.-D.<\/strong> and Chen, T.-C. 2009. Detection and
              identification of thrips-borne tospoviruses by serological and
              nucleic acid tools. APSA-AVRDC Workshop on Tospoviruses and
              Thrips Vectors, Asian Seed Congress 2009 (Bangkok, Thailand,
              Nov. 8, 2009)<\/li>\n
            28. Cheng, Y. F., Li, J. T., Chen, L. F., Huang, L. H., Peng, J.
              C., Yeh, S. D.<\/strong>, and Chen, T. C. 2009. Evaluation of field
              surveys for tospoviruses and potyviruses in the cucurbit-growing
              areas of central and southern Taiwan . Annual meeting of Taiwan
              Plant Protection Society, Abstract, PP-13, p. 39 (Agricultural
              Chemicals and Toxic Substances Research Institute, Taichung,
              Nov. 20, 2009)<\/li>\n
            29. Ho, C. Y., Li, J. T., Kang, Y. C., Chen, Y. T., Fan, Y. S.,
              Huang, L. H., Tseng, H. H., Yeh, S. D.<\/strong>, and Chen, T. C. 2009.
              Emergence of Tomato spotted wilt virus infects Capsicum spp. And
              Zantedeschia sp. crops in Taiwan . Annual meeting of Taiwan
              Plant Protection Society, Abstract, PP-14, p. 40 (Agricultural
              Chemicals and Toxic Substances Research Institute, Taichung,
              Nov. 20, 2009)<\/li>\n
            30. Lin, B. Y., Chen, K. C., Yeh, S. D.<\/strong>, Yu, T. A., Chiang, C.
              H. 2010. The infectivity assays of Papaya ringspot virus
              contained the mutation at the dual coat protein cleavage sites.
              Annual meeting of Taiwan Phytopathology Society, Abstract, SC27
              (National Taiwan University, Taipei, Mar. 6, 2010)<\/li>\n
            31. Fan, Y. H.,Yeh, Y. C., Yeh, S. D.<\/strong>, Chen T. C. 2010.
              Determination and molecular analyses of the NSm genes of Tomato
              yellow ring virus and Peanut chlorotic fan-spot virus. Annual
              meeting of Taiwan Phytopathology Society, Abstract, SC28
              (National Taiwan University, Taipei, Mar. 6, 2010)<\/li>\n
            32. Tsai, W. T., Yeh, S. D.<\/strong> 2010. Production of polyclonal and
              monoclonal antibodies against the NSs proteins of
              Euro-America-Type tospoviruses. Annual meeting of Taiwan
              Phytopathology Society, Abstract, SC30 (National Taiwan
              University, Taipei, Mar. 6, 2010)<\/li>\n
            33. Peng, J. C., Chen, S. K.,Chang, Y. L., Tsai, H. Y., Hu, C.
              C., Yeh, S. D.<\/strong> 2010. The relationship between the retention
              virus rate in whitefly and the Squash leaf curl virus disease
              occurred on melon plants at Tainan area. Annual meeting of
              Taiwan Phytopathology Society, Abstract, V07 (National Taiwan
              University, Taipei, Mar. 6, 2010)<\/li>\n
            34. Kung, Y. J., Lin, S. S., Huang, Y. L., Chen, T. C., Chua, N.
              H., and Yeh, S. D.<\/strong> 2010. Artificial microRNAs targeting the
              conserved motifs of replicase gene conferring transgenic
              resistance to negative-sense ssRNA plant virus. The 12th World
              Congress of the IAPB (St. Louis, Missouri, USA, Jun. 9-11, 2010)<\/li>\n
            35. Huang, C.-H., Hsiao, W.-R., Wang, N.-Y., Huang, C.-W. and
              Yeh, S. D.<\/strong> 2010. Analysis of essential regions of the NSs
              protein of Watermelon silver mottle virus for pathogenicity by
              attenuated strain of Zucchini yellow mosaic virus with point
              mutated in HC-Pro gene. The 12th World Congress of the IAPB (St.
              Louis, Missouri, USA, Jun. 9-11, 2010)<\/li>\n
            36. Yang, C.-F., Yu, T.-A., Yeh, S.-D.<\/strong> 2010. Generation of
              transgenic watermelon resistant to Zucchini yellow mosaic virus
              and Papaya ringspot virus type W. The 12th World Congress of the
              IAPB (St. Louis, Missouri, USA, Jun. 9-11, 2010)<\/li>\n
            37. Wu, P. R., Kang, Y. C., Li, J. T., Yeh, S. D.<\/strong>, and Chen, T.
              C. 2010. Nucleotide sequencing of S RNA of Chrysanthemum stem
              necrosis virus and expression of its nucleocapsid protein for
              antiserum production. Annual meeting of Taiwan Plant Protection
              Society, Abstract, PP-14, p. 47 (National Pingtung University of
              Science and Technology, Pingtung, 10 December 2010).<\/li>\n
            38. Chiu, W. C., Kang, Y. C., Chen, S. M., Chan, S. F., Yeh, S.
              D., and Chen, T. C. 2010. Production of the nucleocapsid
              protein-based antisera for detection of tospoviruses belonging
              to TSWV serogroup. Annual meeting of Taiwan Plant Protection
              Society, Abstract, PP-15, p. 48 (National Pingtung University of
              Science and Technology, Pingtung, 10 December 2010).<\/li>\n
            39. Hsia, C. M., Chan, S. F., Kang, Y. C., Li, J. T., Yeh, S.
              D., and Chen, T. C. 2010. Production of a monoclonal antibody
              against the nucleocapsid protein of Groundnut ringspot virus.
              Annual meeting of Taiwan Plant Protection Society, Abstract,
              PP-16, p. 49 (National Pingtung University of Science and
              Technology, Pingtung, 10 December 2010).<\/li>\n
            40. Wu, H. W., Lin, S. S., Chen, K. C., Huang, C. H., Chua, N.
              H. and Yeh, S. D.<\/strong> 2011. The roles of potyviral gene silencing
              suppressor on symptom severity and host defensive responses.
              Annual meeting of Taiwan Phytopathology Society, Abstract, KS02
              (Chaoyang University of Technology, Taichung, Mar. 25-26, 2011)<\/li>\n
            41. Uthaman, Y., Chen, K. C., Huang, Y. L. Rajagopalan, P. A.,
              Chen. T. C. and Yeh, S. D.<\/strong> 2011. Generation of marker-free
              transgenic plants with broad-spectrum resistance to different
              tospoviruses. Annual meeting of Taiwan Phytopathology Society,
              Abstract, V06 (Chaoyang University of Technology, Taichung, Mar.
              25-26, 2011)<\/li>\n
            42. Hsia, C. M., Chan, S. F., Kang, Y. C., Li, J. T., Yeh, S. D.<\/strong>
              and Chen, T. C. 2011. Epitope analysis of a monoclonal antibody
              broadly reacted with the nucleocapsid proteins of
              Euro-American-type tospoviruses. Annual meeting of Taiwan
              Phytopathology Society, Abstract, V11 (Chaoyang University of
              Technology, Taichung, Mar. 25-26, 2011)<\/li>\n
            43. Wu, C. Y., Korniichuk, I. and Yeh, S. D.<\/strong> 2011. Generation of
              transgenic tobacco plants carrying the conserved regions of
              HC-Pro, CIP, NIb and CP genes for broad-spectrum resistance
              against potyviruses. Annual meeting of Taiwan Phytopathology
              Society, Abstract, SC20 (Chaoyang University of Technology,
              Taichung, Mar. 25-26, 2011)<\/li>\n
            44. Maulidiyah, A. Shen, T. H. and Yeh, S. D.<\/strong> 2011.
              Broad-spectrum transgenic resistance against tospoviruses
              conferred by the expression of a single-chain antibody against
              the common epitope of the gene silencing suppressor. Annual
              meeting of Taiwan Phytopathology Society, Abstract, SC22 (Chaoyang
              University of Technology, Taichung, Mar. 25-26, 2011)<\/li>\n
            45. Yeh, S. D.<\/strong>, Chen, K. C. and Cheng, H. W. 2011. A critical
              amino acid of 6K2 protein of Papaya ringspot virus for inducing
              wilting symptom on papaya plants. 2011 APS-IPPC Joint Meeting,
              Abstract, P994. (Honolulu, Hawaii, USA, Aug. 6-10, 2011)<\/li>\n
            46. Peng, J. C., Chen, T. C. and Yeh, S. D.<\/strong> 2011. Generation of
              broad-spectrum resistance in transgenic tobacco and tomato
              plants against distinct tospovirus species of different
              serogroups. 2011 APS-IPPC Joint Meeting, Abstract, P685.
              (Honolulu, Hawaii, USA, Aug. 6-10, 2011)<\/li>\n
            47. Yeh, S. D.<\/strong> and Kung, Y. J. 2011. Solving the problem of
              sequence homology-independent breakdown of transgenic resistance
              by disarming viral gene silencing suppressor. 2011 APS-IPPC
              Joint Meeting, Abstract, P995. (Honolulu, Hawaii, USA, Aug.
              6-10, 2011)<\/li>\n
            48. Chiang, C. H., Wu, Z. J., Lin, B. Y., Bau, H. J., Chen, K.
              C., Yu, T. A. and Yeh, S. D.<\/strong> 2011. Deletion of the N terminus of
              Papaya ringspot virus larger coat protein disrupt viral systemic
              infection. 2011 APS-IPPC Joint Meeting, Abstract, P998.
              (Honolulu, Hawaii, USA, Aug. 6-10, 2011)<\/li>\n
            49. Yeh, S. D.<\/strong>, Chen, C. C., Raja, J. A. J. and Huang, C. H.
              2011. Cross-protection effectiveness of attenuated variants of
              Turnip mosaic virus with mutations in a conserved motif of the
              N-terminal region of HC-Pro. International Union of
              Microbiological Societies 2011-XV International Congress of
              Virology, Abstract, VI-SY23-6. (Sapporo, Hokkaido, Japan, Sep.
              11-16, 2011)<\/li>\n
            50. Chen, T. C., Li, J. T., Huang, L. H., Weng, J. S., Cheng, Y.
              F., Peng, C. F. and Yeh, S. D.<\/strong> 2011. Host preference of
              Watermelon silver mottle virus and Melon yellow spot virus for
              field watermelon and melon. International Union of
              Microbiological Societies 2011-XV International Congress of
              Virology, Abstract, VI-SY46-6. (Sapporo, Hokkaido, Japan, Sep.
              11-16, 2011)<\/li>\n
            51. Cheng, H. W., Li, J. S., Chen, K. C. and Yeh, S. D.<\/strong> 2011.
              Development of a common epitope of NSs protein of Asia-type
              tospoviruses as a tag for recombinant proteins expressed in
              bacterial and plant viral systems. International Union of
              Microbiological Societies 2011-XV International Congress of
              Virology, Abstract, VI-SY49-3. (Sapporo, Hokkaido, Japan, Sep.
              11-16, 2011)<\/li>\n
            52. Huang, C. H., Chen, K. C., Wu, H. W., Hsiao, W. R. and Yeh,
              S. D. 2011. Analysis of essential regions of NSs protein of
              Watermelon silver mottle virus for gene silencing suppression.
              International Union of Microbiological Societies 2011-XV
              International Congress of Virology, Abstract, VI-PO26-2.
              (Sapporo, Hokkaido, Japan, Sep. 11-16, 2011)<\/li>\n
            53. Yeh, S. D.<\/strong>, Chen, T. C. and Peng, J. C. 2011. Broad-spectrum
              transgenic resistance against different tospoviruses at the
              genus level conferred by the conserved region of L genes.
              International Union of Microbiological Societies 2011-XV
              International Congress of Virology, Abstract, VI-PO23-6.
              (Sapporo, Hokkaido, Japan, Sep. 11-16, 2011)<\/li>\n
            54. Kang, Y. C., Tsai, W. T., Haung, C. H., Li, J. T., Weng, J.
              S., Yeh, S. D.<\/strong> and Chen, T. C. 2011. Prompt identification of
              Asian-type and Euroamerican-type tospoviruses using monoclonal
              antibodies against the common epitopes of NSs proteins.
              International Union of Microbiological Societies 2011-XV
              International Congress of Virology, Abstract, VI-PO46-4.
              (Sapporo, Hokkaido, Japan, Sep. 11-16, 2011)<\/li>\n
            55. Kung, Y. J., You, B. J., Chen, K. C., Huang, C. H., Bau, H.
              J. and Yeh, S. D.<\/strong> 2011. The breakdown of transgenic resistance
              of papaya by super strains of Papaya ringspot virus and the
              solution. 3rd The International Symposium on Papaya, Keynote
              speaker (Chiang Mai, Thailand, Dec. 19-21, 2011)<\/li>\n
            56. Yeh, S. D.<\/strong> 2012. New technology for plant virus control. The
              International Conference on Tropical and Sub-tropical Plant
              Diseases 2012, Keynote speaker (Chiang Mai, Thailand, Feb. 7-10,
              2012)<\/li>\n<\/ol>\n<\/div>\n
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              \u8449\u932b\u6771\u3001\u912d\u6d69\u6587\u3002\u771f\u6838\u751f\u7269\u8868\u73fe\u7cfb\u7d71\u53ca\u5176\u61c9\u7528\u3002\u4e2d\u83ef\u6c11\u570b\u5c08\u5229\u7b2cI 504747\u865f(2015\/10\/21- 2033\/08\/07)\u3002<\/p>\n<\/div>\n

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              \u8449\u932b\u6771 \u6559\u6388 \u91cd\u8981\u5b78\u7d93\u6b77 1983 \u7f8e\u570b\u5eb7\u5948\u723e\u5927\u5b78 (Cornell University) \u690d\u7269\u75c5\u2026<\/p>\n

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