分子植物病原真菌研究室 — _英文版

Fungal Genetics and Molecular Biology; Plant-Microbe Interactions; Bio-agents for Disease Management

Research in my Lab focuses on fungal pathogen-host interactions and disease management with the development of bio-agents.  Current projects include:

(1) Molecular interactions of  Colletotrichum acutatum and chili pepper:  We use genomic, transcrptomic and genetic approaches to analyze the function of candidate effector proteins. We also establish a T-DNA insertion library to identify novel fungal development and/or pathogenicity genes.

(2) Molecular interactions of  Colletotrichum gloeosporioides and mango:  We use genomic, transcrptomic and genetic approaches to identify potential genes involved in fungal pathogenicity and sensing to host factor and various environmental stresses.

(3) Disease resistance in rice to Pythium:  We have identified several potential genes in rice which are involved in  resistance to Pythium. Currently we are working on characterizing their function, mechanisms and applications.

Molecular Plant Pathology, Laboratory of Molecular Plant Pathology, Biopesticides 

1. Chi-Kuan Tu, Wen-Di Huang, Pei-Han Wang, Wei-Lun Lin, Hong-Yue Chen, Sheng-Tsz Rau, Tsu-Cheng Chang, Li-Sen Young, Chih-Li Wang, Miin-Huey Lee* (2024, March) The rice endophytic bacterium Bacillus velezensis LS123N provides protection against multiple pathogens and enhances rice resistance to wind with increase in yield. Biological Control. Volume 192, 2024, https://doi.org/10.1016/j.biocontrol.2024.105507
2. MUZAFFAR, ADNAN; Chen, Yi-Shih; Lee, Hsiang-Ting; Wu, Cheng-Chieh; Le, Trang Thi; Liang, Jin-Zhang; Lu, Chun-Hsien; Balasubramaniam, Hariharan; Lo, Shuen-Fang; Yu, Lin-Chih; Chan, Chien-Hao; Chen, Ku-Ting; Lee, Miin-Huey; Hsing, Yue-Ie; Ho, Tuan-Hua David; Yu, Su-May (2023, Dec). A newly evolved rice-specific gene JAUP1 regulates jasmonate biosynthesis and signaling to promote root development and multi-stress tolerance. Plant Biotechnology Journal. https://doi.org/10.1111/pbi.14276
3. Zun-Jie Syu, Chi-Kuan Tu, Chy-Yu Chen, Shuen-Fang Lo, and Miin-Huey Lee* (2023, Dec). A Large-Scale Hydroponic Evaluation of Rice Mutants for Pythium Resistance. Plant Disease, https://doi.org/10.1094/PDIS-10-23-2179-RE.
4. Jie-Hao Ou, Chang-Hsin Kuo, Yea-Fang Wu, Guo-Cih Lin, Miin-Huey Lee, Rong-Kuen Chen, Hau-Ping Chou, Hsin-Yuh Wu, Sheng-Chi Chu, Qiao-Juan Lai, Yi-Chen Tsai, Chun-Chi Lin, Chien-Chih Kuo, Chung-Ta Liao, Yi-Nian Chen, Yen-Wei Chu, Chi-Yu Chen. 2023. Application-oriented deep learning model for early warning of rice blast in Taiwan. Ecol. Informatics 73: 101950.
5. Chao-Yang Kao, Chun-Ta Wu, Chsin-Hzier Lin, Dai-Keng Hsieh, Huey-Ling Lin, and Miin-Huey Lee*. 2022. The G protein subunit α1, CaGα1, mediates ethylene sensing of mango anthracnose pathogen Colletotrichum asianum to regulate fungal development and virulence and mediates surface sensing for spore germination. Front. Microbiol. 13:1048447.doi: 10.3389/fmicb.2022.1048447
6. Ching-Ling Wang, Mu-Wei Li, Yung-Kuan Chan, Shyr-Shen Yu, Jie Hao Ou, Chi-Yu Chen, Miin-Huey Lee, and Chuen-Horng Lin. 2022. Multi-Scale Features Fusion Convolutional Neural Networks for Rice Leaf Disease Identification. Journal of Imaging Science and Technology. 66(5): 050501-1-050501-12, 2022.
7. Chi-Kuan Tu, Pei-Han Wang, and Miin-Huey Lee*. 2023. The endophytic bacterium Lysobacter firmicutimachus strain 5-7 is a promising biocontrol agent against rice seedling disease caused by Pythium arrhenomanes in nursery trays. Plant Dis. 107: 1075-1086. Doi: 10.1094/PDIS-05-22-1195-RE.
8. Handoko RNS, Tu CK, Ou JH, Chen YN, Lee MH*. First report of Pythium aristosporum causing root rot on rice seedling in Taiwan. Plant Dis. 2022 May 20. doi: 10.1094/PDIS-01-22-0092-PDN. Epub ahead of print. PMID: 35596245.
9. Dai-Keng Hsieh, Shu-Cheng Chuang, Chun-Yi Chen, Ya-Ting Chao, Mei-Yeh Jade Lu, Miin-Huey Lee*, and Ming-Che Shih. 2022. Comparative genomics of three Colletotrichum scovillei strains and genetic analysis revealed genes involved in fungal growth and virulence on chili pepper. Front. Microbiol. 13:818291.doi: 10.3389/fmicb.2022.818291
10. Chia-Chi Kuo, Yung-Chu Lin, Li-Hung Chen, Meng-Yi Lin, Ming-Che Shih and Miin-Huey Lee* (2020, Dec). CaNRT2.1 is required for nitrate but not nitrite uptake in chili pepper pathogen Colletotrichum acutatum. Frontiers in Microbiology 2021 Jan 5;11:613674. doi: 10.3389/fmicb.2020.613674. eCollection 2020.
11. Ka-Tung Leung, Chi-Yi Chen, Bang-Jau You, Miin-Huey Lee*, and Jenn-Wen Huang. 2020. Brown root rot disease of Phyllanthus myrtifolius: the causal agent and two potential biological control agents. Plant Disease.104(11):3043-3053. https://doi.org/10.1094/PDIS-02-20-0412-RE
12. Fang-Yi Yu, Chiu-Min Chiu, Yue-Zhi Lee, Shiow-Ju Lee, Chien-Ming Chou, Bang-Jau You, Dai-Keng Hsieh, Maw-Rong Lee, Miin-Huey Lee*, and Richard M. Bostock. 2020. Polyketide synthase gene expression in relation to chloromonilicin and melanin production in Monilinia fructicola. Phytopathology.110:1465-1475. https://doi.org/10.1094/PHYTO-02-20-0059-R  
13. Ting-Hang Liu, Mohammad A. Yaghmour1, Miin-Huey Lee, Thomas M. Gradziel, Johan Leveau, and Richard M. Bostock. 2019. A roGFP2-based bacterial bioreporter for redox sensing of plant surfaces. Phytopathology. Published Online:16 Dec 2019 https://doi.org/10.1094/PHYTO-07-19-0237-R
14. Pei-Ling Yu, Chih-Li Wang, Pei-Yin Chen and Miin-Huey Lee* 2017. YAP1 homologue-mediated redox sensing is crucial for a successful infection by Monilinia fructicola. Molecular Plant Pathology 18(6): 783–797. DOI: 10.1111/mpp.12438
15. Bang-Jau You, Ni Tien, Miin-Huey Lee, Bo-Ying Bao, Yih-Shyuan Wu, Tsung-Chi Hu & Hong-Zin Lee. 2017. Induction of apoptosis and ganoderic acid biosynthesis by cAMP signaling in Ganoderma lucidum. Scientific Reports 7: 318. doi:10.1038/s41598-017-00281-x
16. Lo, Shuen-Fang; Ho, Tuan-Hua; Liu, Yi-Lun; Jiang, Miring-Jier; Hsieh, KunTing; Chen, Ku-Ting; Yu, Lin-Chih; Lee, Miin-Huey; Chen, Chi-yu; Huang, Tzu-Pi; Kojima, Mikoko; Sakakibara, Hitoshi; Chen, Liang-Jwu; Yu, Su-May (2016, Dec). Ectopic expression of specific GA2 oxidase mutants promotes yield and stress tolerance in rice. Plant Biotechnology. DOI: 10.1111/pbi.12681
17. T. Liu., K. D., Chiu, T. K., Hsieh, J. W., Huang and M. H. Lee* 2015. Development of detached mango leaf-inoculation system for rapid evaluating the efficacy of biocontrol agent on controlling mango anthracnose. Plant Pathol. Bull. 24: 189-199.
18. Chien-Ming Chou, Fang-Yi Yu, Pei-Ling Yu, Richard M. Bostock, Kuang-Ren Chung, Jenn-Wen Huang and Miin-Huey Lee*. 2015. Expression of five endopolygalacturonase genes and demonstration that MfPG1 overexpression diminishes virulence in the brown rot pathogen Monilinia fructicola. PLoS One 10(6):e0132012.
19. Chiu-Min Chiu, Bang-Jau You, Chien-Ming Chou, Pei-Ling Yu, Fang-Yi Yu, Shiah-Mei Pan, Richard M. Bostock, Kuang-Ren Chung and Miin-Huey Lee*. 2013. Redox status-mediated regulation of gene expression and virulence in the brown rot pathogen Monilinia fructicola. Plant Pathology, 62: 809-819
20. De Cal, P. Sandín-Espa˜na, F. Martinez, B. Egüen, C. M. Chiou, M.H. Lee, P. Melgarejoa and D. Prusky. 2013. Role of gluconic acid and pH modulation in virulence of Monilinia fructicola on peach fruit. Postharvest Biology and Technology 86: 418–423.
21. Bang-Jau You*, Miin-Huey Lee, Ni Tien, Meng-Shiou Lee, Hui-Chuan Hsieh, Lin-Hsien Tseng, Yu-Lin Chung, and Hong-Zin Lee. 2013. A Novel Approach to Enhancing Ganoderic Acid Production by Ganoderma lucidum Using Apoptosis Induction. PLoS ONE 8(1):
22. Bang-Jau You, Hong-Zin Lee, Kuang-Ren Chung, Miin-Huey Lee , Mei-Jung Huang, Ni Tien, Chiao-Wei Chan and Yueh-Hsiung Kuo. 2012. Enhanced production of ganoderic acids and cytototoxicity activity by Ganoderma lucidum using solid-medium culture. Bioscience Biotechnology and Biochemistry 76:120270-1-6. IF: 2.292
23. Miin-Huey Lee*, Shiah-Mei Pan, Thieng-Wei Ngu, Po-Sheng Chen, Li-Yuan Wang and Kuang-Ren Chung. 2011. Mutations of β-tubulin codon 198 or 200 indicate thiabendazole resistance among isolates of Penicillium digitatum collected from citrus in Taiwan. International Journal of Food Microbiology 150(2-3): 157-163
24. Po-Sheng Chen, Li-Yuan Wang, You-Jen Chen, Kuo-Ching Tzeng, Shih-Chieh Chang, Kuo-Ching Tzeng, Kuang-Ren Chung^* and Miin-Huey Lee*. 2012. Understanding cellular defence in kumquat and calamondin to citrus canker caused by Xanthomonas citri citri. Physiological and Molecular Plant Pathology. 79: 1-12 (IF:1.377, 96/190=50.5% in Plant Science)
25. Chien-Yao Liao, Mei-Ya Chen, Yu-Ken Chen, Tien-Cheng Wang Zong-Ming Sheu, Ker-Chung Kuo, Pi-Fang Chang, Kuang-Ren Chung and Miin-Huey Lee*. 2012. Characterization of three Colletotrichum acutatum isolates from Capsicum European Journal of Plant Pathology 133:599-608
26. Chien-Yao Liao, Mei-Ya Chen, Yu-Ken Chen, Ker-Chung Kuo, Kung-Ren Chung and Miin-Huey Lee*. Formation of highly branched hyphae by Colletotrichum acutatum within the fruit cuticles of Capsicum spp. Plant Pathology 61: 262–270
27. Miin-Huey Lee, Chiu-Min Chiu, Tatiana Roubtsova, Chien-Ming Chou and Richard M. Bostock*. 2010. Overexpression of a redox-regulated cutinase gene MfCUT1 increases virulence of the brown rot pathogen Monilinia fructicola on Prunus Molecular Plant-Microbe Interactions. 23:176-186
28. You, B. J., Lee, M. H. and Chung, K.R.Gene-specific disruption in the filamentous fungus Cercospora nicotianae using a split-marker approach. Archive Microbiology 191:615-622.
29. You, B. J., Lee, M. H. and Chung, K.R. Production of cercosporin toxin by the phytopathogenic Cercospora fungi is affected by diverse environmental signals. Canadian Journal of Microbiology 54: 259-269
30. Chen, H. Q., Lee, M. H., and Chung, K. R. 2007. Functional characterization of three genes encoding putative oxidoreductases required for cercosporin toxin biosynthesis in the fungus Cercospora nicotianae. Microbiology 153:2781-2790
31. Chen, H. Lee, M. H., Daub, M. E., and Chung, K. R. 2007. Molecular analysis of the cercosporin biosynthetic gene cluster in Cercospora nicotianae. Molecular Microbiology 64(3): 755–770
32. Choquer, M., Lee, M. H., Bau, H. J., and Chung, K. R. Deletion of a MFS transporter-like gene in Cercospora nicotianae reduces cercosporin toxin accumulation and fungal virulence. FEBS Letters 581:489-494
33. Dekkers, K. L., You, B. J., Chen, H. Q., Gowda, V. S., Liao, H. L., Lee, M. H., Ueng, P. P. and Chung K. R. 2007. The Cercospora nicotianae gene encoding dual O-methyltransferase and FAD-dependent monooxygenase domains mediates cercosporin biosynthesis. Fungal genetic and biology 44(5):444-54
34. Lee, M. H. and Bostock, R. M. 2007. Fruit exocarp phenols in relation to quiescence and development of Monilinia fructicola infections in Prunus: a role for cellular redox. Phytopathology 97:269-277
35. Lee, M. H. and Bostock, R. M. 2006. Induction, regulation, and role in pathogenesis of appressoria in Monilinia fructicola. Phytopathology 96:1072-1080
36. Lee, M. H. and Bostock, R. M. 2006. Agrobacterium T-DNA-mediated integration and gene replacement in the brown rot pathogen Monilinia fructicola. Current Genetics 49:309-322
37. Lee, M. H.*, Tzeng, D. D. S. and Hsu, F. S. 2006. Photodynamic effects of methionine-riboflavin mixture on antioxidant proteins. Plant Pathol. Bull. 15:17-24
38. Lee, M. H.* and Tzeng, D. D. S. 2006. Pantoea agglomerans is a potential pathogen causing necrosis on mongo leaf. Plant Pathol. Bull. 15:63-68

1. Kuang-Ren Chung and Miin-Huey Lee. Split marker-mediated transformation and targeted gene disruption in filamentous fungi (invited contribution). Genetic Transformation System in Fungi, Volume 2. (ISBN: ISBN 978-3-319-10503-1 (eBook)). Springer Publishes. Dec, 2014: Chapter 15, p. 175-180.
2. Lee, M. H. 2005. Microscopic, physiological and molecular studies of pathogenesis in Monilinia fructicola, the brown rot pathogen of stone fruits. Ph.D. dissertation. University of California, Davis, USA.

1. William J. Moller Graduate Student Research Award (2006), UC Davis

1. Miin-Huey Lee^,*, Kuan-Ting, Liu, and Jenn-Wen Huang. Development of bio-based strategies to control anthracnose disease on mango. The 8^th International Conference of Clinical Plant Science. Dec. 11, Daegu, Korea. (Invited speech)
2. Miin-Huey Lee*, Chia-Chi Kuo, Yung-Chu Lin, Meng-Yi Lin, Shu-Cheng Chuang, and Ming-Che Shih. 2017. Reverse and forward genetic studies for the pathogenicity and growth of Colletotrichum. 2017 Taiwan-Japan Mycological Research Conference. Oct. 21, Taipei, Taiwan. (Invited speech)
3. Chia-Chi Chang, Kuan-Ting Liu, Jenn-Wen Huang, and Miin-Huey Lee*, 2016. Mango Anthracnose: Pathogenicity and Disease Control. International Symposium on Agricultural Biotechnology, Dec. 12, 2016, NCHU, Taichung, Taiwan. (Invited speech)
4. Chia-Chi Chang, Yung-Chu Lin, Miin-Huey Lee*. 2016. Agrobacterium T-DNA insertions identified genes involved in growth and pathogenicity of Colletotrichum gloeosporioides on mango. 13^th European Conference on Fungal Genetics, April 3-6, Paris, France.
5. Pei-Ling Yu, Chia-Chi Chang, Chiu-Min Chiu, Pei-Yin Chen and Miin-Huey Lee* 2016. The transcription factor MfAP1-mediated redox sensing is crucial for a successful infection by Monilinia fructicola. 13^th European Conference on Fungal Genetics, April 3-6, Paris, France.
6. Kuan-Ting Liu, Jenn-Wen Huang and Miin-Huey Lee^*. 2015. Screening and application of antagonistic bacteria and plant extracts to control anthracnose disease on mango. International Symposium on Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus and Bangkok Campus, Thailand, Dec. 16-20. (Invited speech)
7. Miin-Huey Lee. 2015. Molecular mechanisms involved in the interaction of Monilinia fructicola and Rosaceae. Monilinia Workshop, Nov. 25-26, Lleida, Spain, 2015. (Invited speech)
8. Miin-Huey Lee^*, Shiah-Mei Pan, Dai-Kang Hsieh, Chiu-Min Chiu, and Chia-Chi Chang. 2015. Identification and detection of peach brown rot pathogens in Taiwan. 6th International Conference of Clinical Plant Science 2015, Tokyo University, Tokyo, Japan, Dec. 5.
9. Fang-Yi Yu, Chiu-Min Chiu, Shiow-Ju Lee, Maw-Rong Lee and Miin-Huey Lee*. 2015. Antibiotic activity of the stone fruit pathogen Monilinia fructicola: characterization and identification. 11th International symposium on Biocatalysis and Agricultural Biotechnoloy, Banff National Park, Alberta, Canada, Sept. 13-16. (Invited speech)
10. Miin-Huey Lee, Chein-Yao Liao, Mei-Ya Chen, Yung-Chu Lin and Jia-Fang Ho. 2014. Pathogenicity of Colletotrichum acutatum on chili pepper. International Symposium for Agricultural Biotechnology, Hanoi, Vietnam, Dec. 3-9. (Invited speech)
11. Miin-Huey Lee, Pei-Ling Yu, Chiu-Min Chiu, Fang-Yi Yu, and Richard M. Bostock. 2014. The interactions of Monilinia fructicola and Rosaceae: Redox and 10th International symposium on Biocatalysis and Agricultural Biotechnoloy, Kaohsiung, Taiwan, Oct. 28-31 (Invited speech)
12. Chiu-Min Chiu, Fang-Yi Yu, Richard M. Bostock^* and Miin-Huey Lee. 2014. Functional analysis of genes encoding polyketide synthase in Monilinia fructicola, a causal agent of peach brown rot. 10th International symposium on Biocatalysis and Agricultural Biotechnoly, Kaohsiung, Taiwan, Oct. 28-31
13. Shu-Cheng Chuang, Miin-Huey Lee and Ming-Che Shih. 2014. Genomic and transcriptomic analyses unravel effector repertoire of Colletotrichum acutatum during the infection on Capsicum annuum. 12^th European Conference of Fungal Genetics. Seville, Spain, March 23-27
14. Bostock RM, Liu T-H, Yaghmour M, Leveau J, Lee M-H, Martinez-Garcia PJ, Parfitt DE, Gradziel TM, Crisosto CH. 2014. Brown rot in stone fruits, redox and resistance. Invited abstract and speaker, XVII Congress of the Spanish Society for Phytopathology, Lleida, Spain, October 7-10, 2014. Publications Page 33 of 39
15. Liu T-H, Yaghmour MA, Lee MH, Leveau J, Bostock RM. 2013. Construction and expression of roGFP in Pantoea agglomerans as a bioreporter for host surface redox potential in plant-microbe interactions. Phytopathology, 103: 84-85.)
16. Fang-Yi Yu, Chien-Ming Chou, Pei-Ling Yu, Kuang-Ren Chung, Richard M. Bostock and Miin-Huey Lee*.2013. Differential expression of five endopolygalacturonase genes and functional demonstration of MfPG1 as a negative virulence determinant in the brown rot pathogen Monilinia fructicola. XI International Fungal Biology Conference. Karlsruhe, Germany, September 29 – October 3.
17. Fang-Yi Yu, Chien-Ming Chou, Pei-Ling Yu, Kuang-Ren Chung, Richard M. Bostock and Miin-Huey Lee*.2013. Differential expression of five endopolygalacturonase genes and functional demonstration of MfPG1 as a negative virulence determinant in the brown rot pathogen Monilinia fructicola. XI International Fungal Biology Conference. Karlsruhe, Germany, September 29 – October 3.
18. Miin-Huey Lee. 2012. The interactions of Monilinia fructicola and peach: redox, pathogenicity and polyketides. Monilinia Workshop, Dec. 13-14, Lleida, Spain, 2012. (Invited speech)
19. Pei-Ling Yu, Pei-Yin Chen and Miin-Huey Lee*. 2012. Functional analysis of an oxidative stress-regulated gene MfAP1 from Monilinia fructicola. XV International Congress of Molecular Plant-Microbe Interactions, July 29- August 2, Kyoto, Japan. IS-MPMI 2012 XV International Congress of Molecular Plant-Microbe Interactions program and abstracts, PS04-222.
20. Mei-Ya Chen and Miin-Huey Lee*. 2012. Induction and regulation of highly branched penetration structure of Colletotrichum acutatum. XV International Congress of Molecular Plant-Microbe Interactions, July 29- August 2, Kyoto, Japan. IS-MPMI 2012 XV International Congress of Molecular Plant-Microbe Interactions program and abstracts, PS04-223
21. Chien-Yao Liao and Miin-Huey Lee*. 2012. Formation of highly branched hyphae by Colletotrichum acutatum within the fruit cuticles of Capsicum XV International Congress of Molecular Plant-Microbe Interactions, July 29- August 2, Kyoto, Japan. IS-MPMI 2012 XV International Congress of Molecular Plant-Microbe Interactions program and abstracts, PS04-221.
22. Fang-Yi Yu, Chien-Min Chiou, Pei-Ling Yu, Richard M. Bostock, and Miin-Huey Lee*. 2011.Antimicrobial activity and polyketide synthase gene cloning of the stone fruit pathogen Monilinia fructicola. 26^th Fungal Genetic Conference, March 15-20, 2011, Asilomar, CA. Fungal Genetics reports, vol.58-supplement, abstract No. 27

1. 指導大學部洪譽芳獲得科技部大專生專題研究計畫補助 (2017 – 2018)。
2. 指導研究生陳原諄獲得「2016中華民國植物病理學會年會」，學生口頭發表論文競賽乙組第二名。
3. 指導研究生郭家琪獲得「2015中華民國植物病理學會年會」，學生口頭發表論文競賽乙組第一名。
4. 指導研究生邱秋閔參加2014國際研討會ISBAB (International symposium on Biocatalysis and Agricultural Biotechnoloy) 海報競賽獲得優良研究獎。
5. 指導研究生獲得「2010中華民國植物病理學會年會」，學生口頭發表論文競賽第一名(廖建堯)及第二名(周建銘)。
6. 指導研究生邱秋閔參加美國植物病理年會(APS, 2009)發表論文獲得國科會研究生出國發表論文經費補助。