微生物訊息傳遞研究室

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研究領域說明:

 

1. 微生物訊息傳遞與生物膜形成分子機制探討

微生物分泌並感應細胞外小分子量訊息分子 (small signal molecules),來了解其族群在環境中的密度,並進而調控其生物機能 (:致病因子之產生、生物膜的形成、微生物表面移行性、轉型作用、抗生物質產生及產孢等),此一現象稱為quorum sensing (QS)QS已知可調控多種病原菌之致病因子,在致病過程中扮演極重要角色,因此QS被視為一研發抗菌物質之標的。另亦有報導指出QS亦為拮抗與共生性微生物與其宿主建立交互關係之關鍵,因此干擾微生物間訊息溝通系統,為調控微生物機能之一嶄新且有效策略。本研究室主要在於探討土壤或植物根圈及葉表益生細菌Bacillus spp.Streptomyces spp.、植物病原細菌Xanthomonas spp.,如:柑橘潰瘍病菌 X. axonopodis pv. citri之細胞間訊息傳遞系統及其調控功能與干擾訊息傳遞系統之分子機制,藉以發揮益生菌最大效益與發展防治植物病害之策略。

2. 微生物歧異性調查及植物病害綜合防治技術開發

長期化學防治藥劑的施用與不當的農藥使用,使田間抗藥性病原族群逐漸增加,而利用多元化防治方法之病害綜合管理為控制害物族群,使其低於可被接受之經濟危害水平,同時亦兼顧公眾安全與維持生態平衡之有效策略。其中導入益生性拮抗微生物於作物的栽培,亦為近年來植物病害防治策略之一開發重心。枯草桿菌屬與鏈黴菌屬細菌於自然界中常存在於植物葉表、根圈及土壤,其具產生對環境逆境有抗性之(內生/斷生)孢子及分泌多種酵素、抗生物質、蛋白質、維生素、二次代謝產物及天然聚合物等能力,多種菌株亦具有促進動、植物生長、免疫及防禦反應之功能,因此此兩菌屬菌株常被廣泛應用防治細菌、真菌及線蟲等引起之植物病害、種子保護劑或動物用益生菌,亦常被應用於酵素、蛋白、抗生素等工業發酵生產,可見此兩菌屬為微生物科學與農業科技應用性至為廣泛之ㄧ微生物。本研究室由台灣本土環境中篩選針對柑橘潰瘍病菌、檬果黑斑病菌、水稻白葉枯病、炭疽病菌及灰黴病菌等重要植物病原,具拮抗能力之枯草桿菌屬與鏈黴菌屬菌株,並評估其應用於防治植物病害之潛力,本研究所建立相關應用技術將可提供擬定應用微生物防治植物病害之依據。另針對常用殺蟲及殺菌劑篩選與微生物製劑具親和性之藥劑,作為生物與非生物藥劑混合應用基礎,以降低農藥殘留與減少抗藥性問題產生。所篩選具較佳拮抗潛力及生長特性之之台灣菌株,配合rDNA序列及分子生物學分析,以作為菌株種之鑑定依據,另建立之核酸指紋圖譜(DNA fingerprint),亦可提供未來商品化菌株分子特性資訊。

 

3. 植物病原微生物診斷鑑定技術研發

炭疽病菌Colletotrichum spp.可危害多種國產重要蔬果,嚴重影響作物品質與儲存壽命。炭疽病菌以型態特徵、病徵表現及寄主範圍等特性鑑定不易確認,為因應加入世界貿易組織後,真菌快速檢測及鑑定之需求,本研究室嘗試利用雙股泳動性分析(Heteroduplex mobility assay, HMA) 配合rDNAITS核酸PCR增幅,作為鑑定炭疽病菌依據。另發展藉由測試菌株與一組參考菌株進行HMA分析所形成之HMA分佈圖譜 (將之命名為「異質雙股形式圖譜」 (heteroduplex pattern; HP),來簡化HMA圖譜之判定及增進鑑別力,並期能將HP發展成為「DNA條碼資料庫」以作為未來檢、防疫檢測鑑定炭疽病菌或其他病原微生物之依據。

 

已完成的相關研究:

1. 微生物訊息傳遞與生物膜形成分子機制探討

  •  拮抗性枯草桿菌於葉表之生物膜與其於柑桔潰瘍病菌病害管理之應用

Xanthomonas axonopodis pv. citri引起之柑桔潰瘍病 (citrus bacterial canker) 為影響世界柑桔生產最嚴重之細菌性病害,在美國以及中南美國家即將之列為檢疫病害,並花費不貲極力除滅,現行此病害之防治多仰賴銅劑及抗生素等化學藥劑,然已有報導指出發現抗藥性菌株,因此發展創新、安全及有效之防治策略為時勢所趨。微生物群聚附著於物體表面形成膜狀構造,此泛稱為生物膜,已知生物膜之形成有助於微生物抵抗抗生素等不良環境,且此過程在多種微生物亦已被證實為致病、拮抗或共生作用之關鍵,因此藉由化學藥劑、生物製劑干擾或抑制生物膜形成為研發病害防治策略之嶄新標的。本研究中應用分離自本土介質及根圈土壤,經生理生化及分子指紋圖譜檢測證實其皆歸屬於對人體不具危險性之Bacillus subtilis group,且對柑桔潰瘍病原具拮抗能力之菌株WG6-14TKS1-1,作為發展柑桔潰瘍病防治之生物製劑,其中菌株WG6-14內生孢子生物製劑亦已在台灣登記可供水稻徒長病防治應用。本研究中特別針對枯草桿菌及潰瘍病原於葉表形成生物膜能力與病害發生相關性進行探討,試驗結果證實柑桔潰瘍病菌於葉表生物膜形成及纏存能力與潰瘍病徵表現呈正相關(圖一)。利用共軛焦雷射掃描顯微鏡配合綠螢光蛋白標定及吖啶橙 (acridine orange) 染色法觀察,結果顯示於接種潰瘍病原前一天噴灑枯草桿菌群菌株TKS1-1WG6-14可明顯抑制潰瘍病原於墨西哥萊姆葉表生物膜形成及纏據能力,另可見葉表多由枯草桿菌群菌株所纏據(圖二),且枯草桿菌之應用亦可明顯降低單位葉表面積柑桔潰瘍病斑數(圖三),綜合上述結果顯示利用拮抗性枯草桿菌抑制潰瘍病原於葉表形成生物膜能力可降低柑桔潰瘍病害發生,而達到以拮抗性枯草桿菌生物防治潰瘍病害之目的 [已發表於PLoS ONE (2012) 7(7):e42124已發表於PLoS ONE (2013) 8(4):e62824]

 

圖一: 柑桔潰瘍病菌於聚苯乙烯盤(A)及柑桔葉表(B)生物膜形成與纏存能力正向影響潰瘍病徵表現(C)

 

 

圖二、接種潰瘍病原菌株XW19前一天噴灑枯草桿菌群TKS1-1WG6-14菌株(紅色螢光)可明顯抑制潰瘍病原(綠色螢光)於墨西哥萊姆葉表生物膜形成及纏據能力。

 

圖三、接種潰瘍病原菌株XW19前一天噴灑枯草桿菌群TKS1-1WG6-14菌株於柑桔葉表可明顯降低葉表面積柑桔潰瘍病斑數。

 

  • 新穎二元反應調控蛋白BfdR藉由rpf/DSF訊息傳遞系統調控柑桔潰瘍病菌之生物膜形成能力及毒力

    為釐清X. axonopodis pv. citri於柑桔葉表形成生物膜之分子機制及其與致病作用之關係,本研究中利用跳躍子進行菌株XW19基因體之隨機突變,篩選生物膜形成能力明顯較野生型菌株為低之菌株,其中一突變菌TPH3,跳躍子插入使其喪失功能之基因序列經比對分析,顯示其為二元反應調控蛋白 (two-component response regulator , 命名為BfdR [biofilm formation defective, regulator]),其在非生物性表面以及生物性表面上,形成生物膜能力皆明顯低於野生型菌株,且接種於墨西哥萊姆葉片上,可發現罹病度較野生型菌株為低。利用反轉錄聚合酶鏈鎖反應測定hrpG、rfbC、rpfF、katE等致病相關基因之表現,結果顯示在模擬細胞間質之培養基(XVM2)中,BfdR則可正調控擴散性訊息分子(diffusible signal factor)生合成基因rpfF之表現。[已發表於PLoS ONE (2013) 8(4):e62824

二元反應調控蛋白BfdR突變菌株TPH3其在墨西哥萊姆葉片上形成生物膜能力及罹病度皆明顯低於野生型菌株(TPH2)及互補菌株(TPH5)為低

 

   在模擬細胞間質之培養基(XVM2)中,BfdR則可正調控擴散性訊息分子(diffusible signal factor)生合成基因rpfF之表現。

2. 微生物歧異性調查及植物病害綜合防治技術開發

    由X. axonopodis pv. citri所造成之柑桔潰瘍病為國際間影響柑桔產業最具威脅之細菌性病害,此病害不僅嚴重影響柑柑品質與產量,造成經濟上嚴重損失,亦是世界各國防檢疫單位所重視的病害,然目前尚無推薦可供此病害防治管理應用之生物製劑。本研究旨在篩選對柑柑潰瘍病菌具拮抗能力之台灣本土枯草桿菌屬菌株,並評估其應用於柑柑潰瘍病防治之潛力與探討其可能機制,研究所建立相關應用技術可提供擬定應用微生物防治柑柑潰瘍病之依據。另配合rDNA序列及分子生物學分析,以鑑定收集及篩選之台灣菌株。研究中自中、南部地區所採集本土介質及根圈土壤分離對多種病原具拮抗性細菌,經篩選獲得至少7株對柑桔潰瘍病原具優異拮抗能力之Bacillus菌株TKS1-1OF3-16SP4-17HSP1WG6-14TLB7-7WP8-12,可作為微生物生物製劑發展應用。所篩選菌株經生理生化及分子指紋圖譜檢測證實皆歸屬於對人體不具危險性之B. subtilis 群,且其中對柑桔潰瘍病原具優異拮抗能力之菌株,其分子多型性圖譜明顯與國外菌株不同,此分子指紋圖譜將可作為本土菌株商品化、專利申請或具潛力菌株篩選應用。 [研究成果已發表於PLoS ONE 7(7):e42124]

 

對柑桔潰瘍病菌具優異拮抗活性之菌株TKS1-1WG6-14WP8-12SP4-1716S rDNA分析皆歸屬於對人體不具危險性之B. subtilis 群,而明顯不同於B. cereus 群。

 

具優異拮抗活性之菌株TKS1-1WG6-14WP8-12SP4-17BOXA1R-PCR指紋圖譜及類群關係迥異於其他同屬種標準參考菌株。

 

3. 植物病原微生物診斷鑑定技術研發

炭疽菌Colletotrichum spp.可危害多種國產重要蔬果,嚴重影響作物品質與儲存壽命。炭疽菌以型態特徵、病徵表現及寄主範圍等特性鑑定不易確認,為因應加入世界貿易組織後,真菌快速檢測及鑑定之需求,本研究嘗試利用發展異質雙股泳動性分析(Heteroduplex mobility assay, HMA)配合rDNAITS核酸PCR增幅,作為鑑定炭疽菌依據。經供試菌株rDNAITS增幅產物兩兩進行HMA分析,可將目前測試菌株歸類成CGCACMCLCC5群,分別以C. gloeosporioidesC. acutatumC. musaeC. lindemuthianumC. capsici為其代表之菌株,此分群結果與rDNAITS序列比對相同。另藉由測試菌株與一組參考菌株C. gloeosporioides Cg1C. gloeosporioides Cg16 C. musae Cm1C. gloeosporioides Cg2C. graminicola Cgr1C. lindemuthianum Cl1,進行HMA分析所形成之HMA分佈圖譜 (將之命名為「異質雙股形式圖譜」 (heteroduplex pattern; HP),可將所有炭疽病菌區分成CG1CG2CACMCLCC等六群,其中亦可將僅六個核酸鹽基差別之 C. gloeosporioides 區分成兩亞群CG1CG2。綜合上述試驗結果,HMAHP分析方法配合PCR增幅rDNA ITS區間序列,確可作為炭疽病種階層鑑定與親緣關係研究相當明確的輔助依據。其中HP不僅有助於簡化HMA圖譜之判定及增進鑑別力,並可發展成為DNA條碼資料庫以作為未來檢、防疫檢測鑑定炭疽病菌之依據 [研究成果已發表於J. Phytopath. (2010) 158(1):46-55 & New Biotech. (2011) 28(1):72-78.]

 

 

 

 

研究著作

(A) 期刊論文 Publications In Refereed Journals

Huang, T. P.*, K. M. Lu, and Y. H. Chen. 2013. A novel two-component response regulator links rpf with biofilm formation and virulence of Xanthomonas axonopodis pv. citri. PLoS ONE 8(4):e62824 (SCI, NSC97-2313-B-005-039-MY3 and NSC99-2321-B-005-014-MY3 )

Huang, T. P.* 2013. Regulation of biofilm formation and pathogenicity of citrus canker bacteria and its application in disease management. Plant Pathol. Bull. 22(1):1-11.

Huang, T. P.*, D. D. S. Tzeng, A. C. L. Wong, C. H. Chen, K. M. Lu, Y. H. Lee, W. D. Huang, B. F. Hwang, and K. C. Tzeng. 2012. DNA polymorphisms and biocontrol of Bacillus antagonistic to citrus bacterial canker with indication of the interference of phyllospere biofilm. PLoS ONE 7(7):e42124 (SCI, NSC97-2313-B-005-039-MY3, NSC99-2321-B-005-014-MY3, 99AS-9.2.2-BQ-B2(13) and 100AS-9.2.2-BQ-B2(1))  

Hwang, B. F., I. P. Liu, T. P. Huang. 2012. Gene-environment interaction between interleukin-4 promoter and molds in childhood asthma. Ann. Epidemiol. 22(4):250-256. (SCI) 

Hwang, B. F., I. P. Liu, T. P. Huang. 2011. Molds, parental atopy and pediatric incident asthma. Indoor air 21(6)472-478 (SCI) 

Huang, T. P., , C. H. Chen, Y. H. Lee, B. F. Hwang, D. S. Tzeng, K. C. Tzeng. 2011. Genetic diversity of antagonistic Bacillus subtilis against citrus canker Bacteria. Phytopathology 101:S76. (SCI, NSC97-2313-B-005-039-MY3; NSC99-2321-B-005-014-MY3; 99AS-9.2.2-BQ-B2-13; 100AS-9.2.2-BQ-B2-1) 

Huang, W. D., Y. H. Chiu, T. H. Wang, T. P. Huang, D. S. Tzeng. 2011. The amplification culture of endospore formulation of Bacillus subtilis biofungicide and its use in disease management. Phytopathology 101:S76. 

Tzeng, D. S., W. D. Huang, Y. R. Liang, C. Y. Chen, T. P. Huang, T. L. Lee, W. R. Lai. 2011. The use of arthrospore formulation of antagonistic Streptomyces for the control of diseases caused by Phytophthora species. Phytopathology 101:S180. 

Huang, T. P., Y Yeh and D. D.-S. Tzeng. 2011. Barcode-like heteroduplex DNA pattern as an aid for rapid identification of anthracnose fungi. New Biotech. 28(1):72-78. (SCI, 97AS-14.3.1-BQ-B1(14)) 

Huang, T. P., Y Yeh and D. D.-S. Tzeng. 2010. Heteroduplex mobility assay for identification and phylogenetic analysis of anthracnose fungi. J. Phytopath. 158(1)46-55. (SCI, 97AS-14.3.1-BQ-B1(14)) 

Huang, T. P. and K. C. Tzeng. 2009. Biofilm formation and motility by strains of Xanthomonas axonopodis pv. citri causing differential symptoms on citrus leaves. Phytopathology 99(6 Suppl.):S56 (SCI, NSC 97-2313-B-005-039-MY3)  

Huang, T. P., Y Yeh and D. D.-S. Tzeng. 2008. Identification of anthracnose fungi by heteroduplex mobility assay and heteroduplex pattern. Phytopathology 98(6 Suppl.):S9. (SCI, 97AS-14.3.1-BQ-B1(14)) 

Huang, T. P.* and A. C. L. Wong. 2007. Extracellular fatty acids stimulate flagella-independent translocation by Stenotrophomonas maltophilia. Res. Microbiol. 158(8-9): 702-711 (SCI) 

Huang, T. P. and A. C. L. Wong. 2007. cAMP receptor protein regulated cell-cell communication system mediates expression of a FecA homologue in Stenotrophomonas maltophilia. Appl. Environ. Microbiol. 73(15): 5034-5040 (SCI). 

Huang, T.P., E. B. Somers, and A. C. L. Wong. 2006. Differential biofilm formation and motility associated with lipopolysaccharide/exopolysaccharide-coupled biosynthetic genes in Stenotrophomonas maltophilia. J. Bacteriol. 188(8):3116–3120 (SCI). 

Shimizu, K. J. K. Hicks, T. P. Huang, and N. P. Keller. 2003. Pka, Ras and RGS protein interactions regulate activity of AflR, a Zn(II)2Cys6 transcription factor in Aspergillus nidulans. Genetics 165(3):1095-1104 (SCI). 

*corresponding author

(B) 學術性專刊 Technical Publications

Huang, T. P. and A. C. L. Wong. 2004. Involvement of lipopolysaccharide in biofilm formation by a septic tank system clogger Stenotrophomonas maltophilia. FRI Newsletter 16(4)2-3.

(C) 碩士、博士論文 Thesis

Der-Syh Tzeng, Huang, T. P. et al. 2009. Maximum Residue Limits of Taiwanese Agricultural Products in Major Exported Countries (Regions). Version 7. 362 PP.  ISBN:957-01-9724-2. Agriculture and Food Agency, Council of Agriculture, Executive Yuan, R.O.C. and National Chung-Hsing University.

 Der-Syh Tzeng, Huang, T. P. et al. 2008. Maximum Residue Limits of Taiwanese Agricultural Products in Major Exported Countries (Regions). Version 6. 475 PP.  ISBN:957-01-9724-2. Agriculture and Food Agency, Council of Agriculture, Executive Yuan, R.O.C. and National Chung-Hsing University.

 Biofilm formation and cell-cell signaling in Stenotrophomonas maltophilia. 2007. University of Wisconsin-Madison, USA. (Ph. D dissertation)

Application of rDNA internal transcribed spacer (ITS) sequence characteristics as an aid for the identification and phylogenetic study of Colletotrichum spp. 1999. National Chung-Hsing University. (Master thesis)

 

(D) 研討會論文及發表 Conference Presentations

Huang, T.-P., Chen, Y.-H. Huang, S.-Y. Chang, H.-Y. Tzeng, D.-S. 2015. Management of strawberry anthracnose by application of Bacillus subtilis endospore formulations and potential resistant cultivars. XVIII. International Plant Protection Congress. 24-27 August, 2015, Berlin, Germany. (P N-CCO 29)

黃姿碧 2013。柑桔潰瘍病菌生物膜形成機制與病害管理應用. 國立成功大學熱帶植物科學研究所102學年專題演講系列活動6 (Invited speaker) 

黃姿碧*、呂冠民、陳郁璇。2013柑桔潰瘍病菌生物膜形成機制與病害管理應用。2013年海峽兩岸植物病理學青年科學家論壇。北京,中國大陸。 

Yu-Hsuan, Chen and Huang, T. P. * 2013. A two component response regulator BfdR regulatesbiofilm formation and virulence through the rpf /DSF system in Xanthomonas axonopodis pv. citri. Annual Meeting of Taiwan Phytopathological Society, Taichung, Taiwan, April 26-27, 2013. (SC32 ) 

Huang, T. P. * and K. M. Lu. 2012. The role of two-component response regulator in biofilm formation and pathogenicity by Xanthomonas axonopodis pv. citri. IS-MPMI 2012 XV International Congress. Kyoto, Japan (PS14-537) 

Huang, T. P. *, C. H. Chen, Y. H. Lee, B. F. Hwang, D. D. S. Tzeng, and K. C. Tzeng. 2011 Genetic diversity of antagonistic Bacillus subtilis against citrus canker bacteria. 2011 APS-IPPC Joint Meeting. Honolulu, Hawaii, USA. (9-P) 

Tzeng, D. S, W. D. Huang, Y. R. Liang, C. Y Chen,.T. P. Huang, T. L. Lee, and W. R.. Lai. 2011. The use of arthrospore formulation of antagonistic Streptomyces for the control of diseases caused by Phytophthora species. 2011 APS-IPPC Joint Meeting. Honolulu, Hawaii, USA. (803-P) 

Huang, W. D., Y. S. Chiu, T. H Wang, T. P. Huang, and D. S. Tzeng. 2011.The amplification culture of endospore formulation of Bacillus subtilis biofungicide and its use in disease management. 2011 APS-IPPC Joint Meeting. Honolulu, Hawaii, USA. (748-P) 

呂冠民、曾德賜、曾國欽、黃姿碧*2011。二元反應調控蛋白對柑桔潰瘍病菌於葉表形成生物膜與致病性之影響。中華民國植物病理學會九十九年度年會。台中市,台灣。(SC02) 

陳金堯、黃文的、李宗倫、黃姿碧、賴文瑞、梁瑩如、曾德賜。2011。鏈黴菌Streptomuces griseobrunneus S3 菌株活孢子製劑在疫病菌危害防治上之應用性。中華民國植物病理學會九十九年度年會。台中市,台灣。(B01) 

Huang, T. P. 2010. Development of molecular techniques for diagnosis and identification of fungal pathogen. 2010 Symposium on Frontiers of Plant Science. Nantou, Taiwan (Invited speaker). 

Huang, T. P. * 2010. Biofilm formation by Xanthomonas axonopodis pv. citri causing citrus canker. 2010 110th ASM General Meeting, San Deigo, CA, USA (I-1731). 

Huang, T. P. * and K. C. Tzeng. 2009. Biofilm formation and motility by strains of Xanthomonas axonopodis pv. citri causing differential symptoms on citrus leaves. 2009 APS Annual Meeting, Portland, OR, USA (P-599). 

黃姿碧. 2009. 看誰在說話 - 微生物間之溝通. 生物物理Journal Club,中興大學. (Invited speaker)   

Huang, T. P. 2009. Microbial communication and its application in disease management. 97 Taiwan Phytopathological Society Conference. Taichung, Taiwan. (Invited speaker) 

Huang, T. P. 2008. Cell-cell communication in Stenotrophomonas maltophilia. The13th Conference on Bacteriology. Taipei, Taiwan. (Invited speaker) 

Huang, T. P. *, Y Yeh and D. D.-S. Tzeng. 2008. Identification of anthracnose fungi by heteroduplex mobility assay and heteroduplex pattern. 2008 APS Centennial Meeting, Minneapolis, MN, USA (P-42).  

Huang, T. P. 2007. Wireless communication in a sand-clogging bacterial pathogen. Food Research & Education Seminar Highlight Seminar. (Invited speaker) 

Huang, T. P. 2006. Biofilm formation and cell-cell signaling in a septic tank system clogger Stenotrophomonas maltophilia. Department of Food Science Seminar. 

Huang, T. P., E. Somers, and A. C. L. Wong. 2005. Lipopolysaccharide/ exopolysaccharide biosynthetic genes are associated with biofilm formation and motility by Stenotrophomonas maltophilia WR-C. 105th American Society for Microbiology, ASM, General Meeting, Atlanta, GA, USA (J-013) and Food Research Institute, FRI, Annual Meeting. 

Huang, T. P. and A. C. L. Wong. 2004. Involvement of a TonB-dependent ferric citrate receptor FecA in biofilm formation by a septic system clogger Stenotrophomonas maltophilia. 104th ASM General Meeting, New Orleans, LA (Q-215) and FRI Annual Meeting.  

Huang, T. P. 2004. Mechanisms of biofilm formation by a septic tank system clogger Stenotrophomonas maltophilia. Department of Food Science Seminar. 

Huang, T. P. and A. C. L. Wong. 2003. Involvement of lipopolysaccharide in biofilm formation by a septic system clogger Stenotrophomonas maltophilia. 3rd ASM Conference on Biofilms, British Columbia, Victoria, Canada (143B). 

Huang, T. P. and N. P. Keller. 2002. RasA-mediated signaling negatively regulates sterigmatocystin production in Aspergillus nidulans. FRI Annual Meeting, Madison, WI, USA.

 

(E) 專書及專書章節

曾德賜、黃姿碧等. 2010. 台灣農產品主要外銷國(地區)殘留農藥容許量手冊。 第八版,350 PP.  ISBN: 957-01-9724-2. 行政院農業委員會農糧署暨國立中興大學編印. 

曾德賜、黃姿碧等. 2009. 台灣農產品主要外銷國(地區)殘留農藥容許量手冊。 第七版,362 PP.  ISBN:957-01-9724-2. 行政院農業委員會農糧署暨國立中興大學編印.

曾德賜、黃姿碧等. 2008. 台灣農產品主要外銷國(地區)殘留農藥容許量手冊。 第六版,475 PP.  ISBN:957-01-9724-2. 行政院農業委員會農糧署暨國立中興大學編印

Biofilm formation and cell-cell signaling in Stenotrophomonas maltophilia. 2007. University of Wisconsin-Madison, USA. (博士論文

Application of rDNA internal transcribed spacer (ITS) sequence characteristics as an aid for the identification and phylogenetic study of Colletotrichum spp. 1999. National Chung-Hsing University. (碩士論文)

 

進行中之研究計畫

2013

Mechanisms of biofilm formation and pathogenesis by citrus canker bacteria and its application in biocontrol. (Ministry of Science and Technology)

 Product development and industrialization of the microbial pesticide - Bacillus subtilis TKS1-1. (Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture, Executive Yuan)