分子植物逆境研究室

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張老師獲得美國普渡大學(Purdue University)植物生理學博士,研究領域和植物對逆境的反應與對病原菌的防禦機制相關。主要探討植物在乾旱、高低溫、高鹽等環境逆境或受病原菌感染等生物逆境之下,植物的生理生化與基因表現的變化。其一為探討水稻第一族及第二族低分子量熱休克蛋白的基因表現與其蛋白質的保護功能。其二為開發重要作物土傳病原真菌Fusarium spp.(尤其是感染不同作物的Fusarium oxysporum真菌)的分子偵測技術及其應用,部份研究成果並已申請專利中。其三為作物防禦相關基因之選殖與其產物的功能分析,尤其針對西瓜抗蔓割病與辣椒抗炭疽病的抗性機制有較深入的探討。

一、開發重要作物尖鐮孢菌及鐮孢菌屬菌株的分子檢測技術

1. 目前已由全台各地收集之供試菌株共計208株,其中包括尖鐮孢菌(Fusarium oxysporum)菌株183株、其他鐮孢菌屬(Fusarium spp.)菌株14株、非鐮孢菌之其它作物病原菌11株。

2. 由香蕉黃葉病菌(F. oxysporum f. sp. cubense)核酸進行「隨機增幅核酸多型性分析(random amplified polymorphic DNA, RAPD)」,增幅得一約400 bp的專一性片段,經選殖及解序後,依此序列設計之專一性引子對,在適當的「聚合酵素連鎖反應(polymerase chain reaction, PCR)」條件下可增幅出特定片段,並可由南方雜合分析確定其對香蕉黃葉病菌生理小種第四型具專一性。相關成果已發表於SCI期刊(Lin et al., 2009),並申請專利中。

3. 由西瓜蔓割病菌(F. oxysporum f. sp. niveum)核酸進行RAPD增幅得一約410 bp的專一性片段,經選殖及解序後,依此序列設計之專一性引子對,在適當的PCR條件下可增幅出特定片段,並可由南方雜合分析確定其對西瓜蔓割病菌具專一性。目前正依此片段開發西瓜蔓割病菌之「快速分子檢測技術」。相關成果申請專利中。

4. 由番茄萎凋病菌(F. oxysporum f. sp. lycopersici)核酸進行RAPD增幅出一約1.41 kb的特異性片段,經選殖核酸序列,完成解序,目前正依此序列設計專一性引子對進行後續測試。

5. 另已取得專一性引子對,在所有供試Fusarium spp.菌株經由PCR技術分析後,可增幅出一約160 bp的專一性片段。

6. 利用前人發表針對F. oxysporum 具專一性之寡核酸片段Fox1為引子,於本研究室測試搭配ITS4引子對進行PCR,所得約190 bp的特定片段對F. oxysporum的菌株DNA具專一性。

7. 已由測試得知Shimazu等學者(2005)發表的STS專一性分子標誌並不完全適用於檢測台灣萵苣萎凋病菌(F. oxysporum f. sp. lactucae, Fola)菌株,另Pasquali等學者(2007)所發表針對Fola生理小種第一型的分子標誌也無法區分台灣的生理小種第一型與新生理小種(洪等人,2008),故目前正在開發檢測台灣Fola及其生理小種之分子標誌。

二、西瓜抗蔓割病之遺傳及抗性機制研究

1. 西瓜蔓割病抗性遺傳分析:

本研究室於91-93年度國科會計畫中,已利用RAPD技術,針對西瓜抗蔓割病(Fusarium wilt of watermelon)的JSB品系與其同源感病的Sugar Baby品種所純化的模板DNA,進行多型性DNA之篩選。由300個逢機引子,選獲16個多型性片段,再經1-3次篩選,最後獲得兩個引子可在抗病JSB樣品中分別增幅出異於感病Sugar Baby樣品的898 bp及800 bp條帶。經進一步研究發現此兩片段可作為西瓜抗蔓割病篩選的RAPD分子標誌(Lin, 2003; Chen, 2004),並已將相關成果發表於SCI期刊(Lin et al., 2009)。

2. 西瓜抗蔓割病抗性機制研究:

本研究室利用上述JSB品系與其同源感病的Sugar Baby品種利用反轉錄聚合酵素連鎖反應(reverse transcription-polymerase chain reaction, RT-PCR)法、抑制劑處理法與組織切片法等技術探索西瓜抗蔓割病之可能的機制,相關成果已發表於SCI期刊(Chang et al., 2008)。

三、辣椒防禦相關基因之選殖與定性

本研究室已利用網路上已發表的基因序列,分別設計出針對番茄及辣椒等茄科作物之防禦相關基因引子對,且利用所設計的引子,對水楊酸(salicylic acid, SA)處理或傷口處理後之番茄與辣椒cDNA進行各防禦相關基因之增幅與選殖。目前已將番茄PR1 (pathogenesis-related protein 1)、PR2PR3PR5PAL (phenylalanine ammonia lyase)、NPR1(nonexpressor of pathogenesis-related genes 1)與NIF1(NPR1-interacting factor)以及辣椒PR1 (pathogenesis-related protein 1)、PR2PR5PALNPR1NIF1(NPR1-interacting factor)等個別基因之部分片段選殖並解序完成,並比對確認為所求之基因片段。且已成功取得NPR1此重要誘導抗性調控基因之全長基因序列,可作為後續基因轉殖之材料。現階段正分析辣椒果實於接種辣椒炭疽病菌(Colletotrichum acutatum)後各個防禦相關基因(PRsPALNPR1NIF1)之表現情形。並初步利用「炭疽病菌-辣椒果實」之系統,探討抗、感病辣椒在接種炭疽病菌後可能之抗病因子,及寄主內防禦相關基因之表現差異。結果顯示抗、感病辣椒成熟(紅)果及未成熟(綠)果以傷口接種或無傷口接種後炭疽病病徵的發展明顯有所差異,並得知AVRDC PBC932為最抗病之辣椒種原。在抑菌物質分析的部分,則發現辣椒果實之水萃取物雖會促進炭疽病菌孢子發芽及菌落生長,但以乙酸乙酯萃取的萃取物卻具有抑制菌落生長之活性。另外,利用北方雜合法分析比較於C. acutatum接種前後,抗、感病性之辣椒組織內防禦相關基因(PR1PR2PR3PR5PALNIF1)之表現情形。由結果發現各供試防禦相關基因(包含PR2PR3PR5PAL)之表現在抗病辣椒(AVRDC PBC932種原)比在感病辣椒(群香品種)中更為顯著,本研究室正更進一步分析了解辣椒中各個防禦相關基因表現情形與辣椒抗病機制之關連性,並以「Differential Display-Reverse Transcriptase-Polymerase Chain Reaction, DD-RT-PCR」與「cDNA-Amplified Fragment Length Polymorphism, cDNA-AFLP」等技術選殖在「辣椒-炭疽病菌」之親和性與不親和性反應中,可能參與抗性(或耐性)之基因,相關成果不日即可發表於期刊上,將來並可利用遺傳工程技術開發出可持續表現此些基因且對病原菌具有抗性之轉基因辣椒或茄科作物,並可加速抗炭疽病作物育種之時程,本研究結果對未來開發適當之炭疽病防治策略將有很大的助益,並可作為植物防疫及抗病育種之重要參考依據。

四、水稻熱休克蛋白、PR1及PR5等抗逆境相關基因之表現及其蛋白質累積之研究與其和水稻抗(耐)逆境之分析(部分成果已發表於SCI期刊,Chang et al., 2007)

1. 利用水楊酸(salicylic acid)、切傷(wounding)及熱處理(41℃)水稻幼苗,並以北方點漬法分析可知水稻第二族小分子量熱休克蛋白(sHSP-CII)在此三逆境下會被誘導。

2. 利用立枯絲核菌(Rhizoctonia solani R96)接種一個月大的水稻植株,再以RT-PCR法進行分析,可知水稻第一族小分子量熱休克蛋白基因Oshsp16.9BOshsp16.9COshsp17.0基因可被立枯絲核菌接種而誘導,推測sHSP-CI基因的表現在植物對抗病原菌之侵入感染過程中可能扮演某種相關的角色。

3. 利用分離自水稻的第二族小分子量熱休克蛋白基因Oshsp18.0-II作為探針,以北方點漬法觀察到水稻幼苗在熱逆境(41℃)下Oshsp18.0-II會大量表現;且將重組後的Oshsp18.0-II融合蛋白大量表現於大腸桿菌(Escherichia coli)體內,可以提高大腸桿菌在高溫下的存活率以及減少其在紫外光下的傷害。

4. 利用熱處理、紫外光處理、過氧化氫處理水稻幼苗,再以西方點漬法觀察蛋白質累積,可知大分子量熱休克蛋白HSP90、HSC70 (HSP73)、HSP60 與第一族小分子量熱休克蛋白(sHSP-CI)在41℃下會被大量誘導。HSP60在紫外光處理下會被誘導;而sHSP-CI 則在過氧化氫處理時會被誘導。

5. 以西方點漬法觀察蛋白質累積,得知水稻在種子成熟過程中HSP60在早期會累積而到後期會逐漸減少;但sHSP-CI則在種子成熟過程之中後期才開始被誘導出來,到成熟時達到最大量;另在水稻種子發芽過程中,HSP60會逐日增加,但sHSP-CI卻會隨著發芽天數而逐漸減少。

6. 將水稻幼苗先以非致死溫度(41℃)預熱處理,再於室溫下回復4小時使HSPs大量累積後,最後置於高溫(55℃)下觀察其後天耐熱性(acquired thermotolerance),由電解質滲漏度與氧化四唑(TTC)染色之結果,可知預熱處理水稻幼苗可使其對後續之熱逆境具有些微的保護性。

7. 已由水稻分離出PR1及PR5等逆境相關蛋白之基因,並以北方點漬法觀察水稻幼苗在熱逆境(41℃)下並不會誘導此二基因之表現;但在立枯絲核菌(Rhizoctonia solani R96)接種的水稻植株中,以北方點漬法或RT-PCR 進行分析,可知PR1 (pathogenesis-related protein 1) 及PR5等基因會受立枯絲核菌接種而誘導其表現。

相關著作:

(A) 期刊論文

      黃盈潔、鍾文鑫、張碧芳、黃振文(2015年07月)。奈米材料抑制動物與植物病原菌生長的功效。永續農業,36:23-30。(台灣)

      張碧芳、黃振文、鍾文鑫、劉詠汶(2015年07月)。微奈米鈣矽對水稻生長和 保護的效果。永續農業,36:13-22。本人為第一作者、通訊作者。(台灣)

      Chang TH, YH Lin, KS Chen, JW Huang, SC Hsiao, and PFL Chang (2015, Mar). Cell wall reinforcement in watermelon shoot base related to its resistance to Fusarium wilt caused by Fusarium oxysporum f. sp. niveum. Journal of Agricultural Science, 153: 296-305. (SCI, 3/56, AGRICULTURE, MULTIDISCIPLINARY). 本人為通訊作者. (NEW YORK, NY, USA)

      Lin YH, PJ Lai, TH Chang, YL Wan, JW Huang, JH Huang, and PFL Chang*. 2014. Genetic diversity and identification of race 3 of Fusarium oxysporum f. sp. lactucae in Taiwan. European Journal of Plant Pathology 140(4): 721-733. (SCI) (ABC) (SCI: Horticulture 8/32, IF= 1.610)

      Lin YH, CC Su, CP Chao, CY Chen, CJ Chang, JW Huang*, and PFL Chang*. 2013. A molecular diagnosis method using real-time PCR for quantification and detection of Fusarium oxysporum f. sp. cubense race 4. European Journal of Plant Pathology 135(2): 395-405. (SCI journal) (IF= 1.610) (SCI) (ABC) (SCI: Horticulture 8/32, IF= 1.610)

      Chang PFL*, WK Huang, YH Lin, Y Chen, and TH Chang. 2012. Protective function of the recombinant Oshsp18.0-CII protein, a class II small heat shock protein of rice, in Escherichia coli. Botanical Studies 53:291-299. (SCI) (ABC) (SCI: Plant Science 129/197, IF= 0.864)

      Liao CY, MY Chen, YK Chen, TC Wang, ZM Sheu, KC Kuo, PFL Chang, KR Chung, and MH Lee*. 2011. Characterization of three Colletotrichum acutatum isolates from Capsicum spp. European Journal of Plant Pathology 133(3): 599-608. (SCI) (SCI: Horticulture 8/32, IF= 1.610)

      Hwang SG , NC Lin, YY Hsiao, CH Kuo, PFL Chang, WL Deng, MH Chiang, HL Shen, CY Chen, and WH Cheng*. 2012. The Arabidopsis short-chain dehydrogenase/reductase 3, an ABSCISIC ACID DEFICIENT 2 homolog, is involved in plant defense responses but not in ABA biosynthesis. Plant Physiology and Biochemistry 51(2): 63-73. (SCI journal) (SCI: Plant Science 40/197=0.203, IF=2.78)

      Lin, YH, HW Chen, and PFL Chang*. 2011. Development and application of microbial agents. National Chung Hsing University of Agriculture. 78: 2-6. (In Chinese)

林盈宏、陳和緯、張碧芳。2011。微生物源製劑的發展與應用。興大農業78: 2-6

      Chen HW, YH Lin, JW Huang, and PFL Chang*. 2010. Effect of Bacillus mycoides on seedlings growth of lettuce. Plant Pathology Bulletin 19(2): 157-165. (In Chinese with abstract in English). (99AS-9.3.1-BQ-B2(6))

      Lin YH, KS Chen, JY Chang, YL Wan, CC Hsu, JW Huang, and PFL Chang*. 2010. Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp. niveum in Taiwan. New Biotechnology 27(4): 409-418. (SCI journal) ( Biotechnology & applied microbiology, 99/160=0.618, IF=1.71) (NSC98-2313-B-005-025-MY3, NSC99-2622-B-005-006-CC2)

  Chiu, EYH**, YH Lin**, W Wu, Q Song, PFL Chang*, LY Gao, CC Chou, and PP Ueng*. 2010. Alternative splicing and genetic diversity of the white collar-1 (wc-1) gene in cereal Phaeosphaeria pathogens. European Journal of Plant Pathology 127(7): 351-363. (SCI journal) (SCI: Horticulture 8/32, IF= 1.610) **Chiu, EYH and YH Lin contributed equally.

  Lin YH, JY Chang, ET Liu, CP Chao, JW Huang, and PFL Chang*. 2009. Development of a molecular marker for specific detection of Fusarium oxysporum f. sp. cubense race 4. European Journal of Plant Pathology 123(3): 353–365. (SCI journal) (SCI: Horticulture 8/32, IF= 1.610) (90AS-6.3.1-BQ-B2(6), 91AS-7.3.1-BQ-B2(3), 93AS-1.9.2-BQ-B1(1), 96AS-4.1.2-IC-I1(2), 97AS-4.1.2-IC-I1(6))

  Wang CL, PFL Chang, YH LIN, A Malkus, LY Gao, and PP Ueng*. 2009. Group I introns in small subunit ribosomal DNA (SSU-rDNA) of cereal Phaeosphaeria species. Botanical Studies 50(2): 137–147. (SCI journal) (SCI: Plant Science 129/197, IF= 0.864)

  Lin YH, KS Chen, TD Liou, JW Huang*, and PFL Chang*. 2009. Development of a molecular method for rapid differentiation of watermelon lines resistant to Fusarium oxysporum f. sp. niveum. Botanical Studies 50(3): 273-280. (SCI journal) (SCI: Plant Science 129/197, IF= 0.864). (90AS-2.1.2-BQ-B7, NSC 91-2313-B-005-094, NSC 92-2313-B-005-054, NSC 93-2313-B-005-080)

  Chiu YHE, PFL Chang, LY Gao, CC Chou, and PP Ueng*. 2009. Genetic diversity of the white collar-2 (wc-2) gene in cereal Phaeosphaeria pathogens. Plant Pathology Bulletin 18(3): 175-183.

  Lin YH, and PFL Chang*. 2008. The role of Arabidopsis NPR1 protein in plant induced systemic disease resistance. Plant Pathology Bulletin 17: 101-110. (In Chinese with abstract in English).

  Hong CF, PFL Chang, JL Huang, YL Wan, and JW Huang*. 2008. Identification for Physiological Races of Fusarium oxysporum f. sp. lactucae and Screening of Lettuce Cultivars Resistant to Fusarium Wilt. Plant Pathology Bulletin 17: 233-242. (In Chinese with abstract in English). (95AS-13.3.1-BQ-B1(6), 96AS-14.3.1-BQ-B1(6), 97AS-14.3.1-BQ-B1(11))

  Chang PFL*, CC Hsu, YH Lin, KS Chen, JW Huang and TD Liou. 2008. Histopathology comparison and phenylalanine ammonia lyase (PAL) gene expressions in Fusarium wilt infected watermelons. Australian Journal of Agricultural Research 59(12): 1146-1155. (SCI journal) (SCI: Agriculture, Multidisciplinary 17/57, IF= 1.133) (2008後改名為Crop & Pasture Science) (90AS-2.1.2-BQ-B7, NSC 91-2313-B-005-094, NSC 92-2313-B-005-054, NSC 93-2313-B-005-080)

  Chang PFL*, TL Jinn, WK Huang, Y Chen, HM Chang and CW Wang. 2007. Induction of a cDNA clone from rice encoding a class II small heat shock protein by heat stress, mechanical injury, and salicylic acid. Plant Science 172(1): 64-75. (SCI journal) (SCI: Plant Science 35/197, IF= 2.922) (NSC88-2311-B-005-042, NSC88-2311-B-005-022, NSC89-2311-B-005-159).

  Wang CL, A Malkus, SM. Zuzga, PFL Chang, BM Cunfer, E Arseniuk, and PP Ueng. 2007. Diversity of the tri-functional histidine biosynthesis gene (his) in cereal Phaeosphaeria species. Genome 50(6): 595-609. (SCI journal) (SCI: Biotechnology & applied microbiology 101/ 160, IF= 1.668)

  Malkus A, PFL Chang, SM Zuzga, K Chung, J Shao, BM Cunfer, E Arseniuk and PP Ueng*. 2006. RNA polymerase II gene (RPB2) encoding the second largest protein subunit in Phaeosphaeria nodorum and P. avenaria. Mycological Research 110(10): 1152-1164. (SCI journal) (SCI: Mycology 10/23, IF= 2.082)

21. Hong CF, PFL Chang, JY Chang, and JW Huang*. 2006. Identification for the Causal Agent of Caspia Anthracnose and Its Pathogenicity Tests. Plant Pathology Bulletin 15: 241-249. (In Chinese with abstract in English).

  Malkus A, E Reszka, CJ Chang, E Arseniuk, PFL Chang and PP Ueng*. 2005. Sequence diversity of β-tubulin (tubA) gene in Phaeosphaeria nodorum and P. avenaria. FEMS Microbiology Letters 249: 49-56. (SCI journal) (SCI: Microbiology 71/116, IF= 2.049).

  Chen KS, TD Liou, PFL Chang and JW Huang*. 2003. Selection for resistance of watermelon varieties (lines) to Fusarium wilt and their genetic analysis of inheritance. Plant Pathology Bulletin 12: 173-180. (In Chinese with abstract in English). (NSC 91-2313-B-005-094, NSC 92-2313-B-005- 054)

  Chen KS, PFL Chang, TD Liou and JW Huang*. 2003. Identification of physiological races of Fusarium oxysporum f. sp. niveum and breeding for Fusarium wilt-resistant watermelon line. Plant Pathology Bulletin 12: 247-254. (In Chinese with abstract in English). (NSC 91-2313-B-005-094, NSC 92-2313-B-005- 054)

  Chang PFL, CY Huang, FC Chang, WC Lin, TS Tseng and CY Lin* 2001. Isolation and characterization of the third gene encoding a 16.9 kDa class I low-molecular-mass heat shock protein, Oshsp16.9C, in rice. Botanical Bulletin of Academia Sinica 42: 85-92. (SCI journal) (NSC 84-2311-B-002-007-B01, NSC 85-2311-B-002-010-B13). (2006後改名為Botanical Studies)

  Guan JC, FC Chang, TS Tseng, PFL Chang, KW Yeh, YM Chen and CY Lin* 1998. Structure of rice genes encoding three class-I low-molecular-mass heat-shock proteins (accession nos. U83669, U83670, U83671) (PGR 98-178). Plant Physiology 118: 1101. (SCI journal) (SCI: Plant sciences 8/197, IF=6.555) (NSC 84-2311-B002-030)

  Chang PFL, Y Xu, ML Narasimhan, KT Cheah, MP DUrzo, B Damsz, AK Kononowicz, L Abad, PM Hasegawa and RA Bressan*. 1997. Induction of pathogen resistance and pathogenesis-related genes in tobacco by a heat-stable Trichoderma mycelial extract and plant signal messengers. Physiologia Plantarum 100: 341-352. (SCI journal) (SCI: Plant sciences 24/197, IF=3.656).

  Jinn TL, PFL Chang, YM Chen, JL Key and CY Lin. 1997. Tissue type-specific heat shock response and immunolocalization of class I low molecular weight heat shock proteins in soybean. Plant Physiology 114: 429-438. (SCI journal) (SCI: Plant sciences 8/197, IF=6.555) (NSC 80-0211-B002-18 & NSC 81-0211-B002-13).

  Yeh CH, PFL Chang, KW Yeh, WC Lin, YM Chen and CY Lin* 1997. Expression of a gene encoding a 16.9 kDa heat-shock protein, Oshsp16.9, in Escherichia coli enhances thermotolerance. Proceedings of the National Academy of Sciences of the United States of America 94: 10967-10972. (SCI journal) (SCI: Multidisciplinary sciences 4/56, IF=9.737) (NSC 83-0211-B002-232 & NSC 84-2311-B002-030).

  Chang PFL, Y Xu, ML Narasimhan, KT Cheah, MP DUrzo, B Damsz, AK Kononowicz, L Abad, PM Hasegawa and RA Bressan* 1997. Induction of pathogen resistance and pathogenesis-related genes in tobacco by a heat-stable Trichoderma mycelial extract and plant signal messengers. Physiologia Plantarum 100: 341-352. (SCI journal) (SCI: Plant sciences 24/197, IF=3.656).

  Chang PFL, B Damsz, AK Kononowicz, M Reuveni, Z Chen, Y Xu, K Hedges, CC Tseng, NK Singh, ML Binzel, ML Narasimhan, PM Hasegawa and RA Bressan*. 1996. Alterations in cell membrane structure and expression of a membrane-associated protein after adaptation to osmotic stress. Physiologia Plantarum 98: 505-516. (SCI journal) (SCI: Plant sciences 24/197, IF=3.656).

  Chang PFL, KT Cheah, ML Narasimhan, PM Hasegawa and RA Bressan*. 1995. Osmotin gene expression is controlled by elicitor synergism. Physiologia Plantarum 95: 620-626. (SCI journal) (SCI: Plant sciences 24/197, IF=3.656)

  Zuker A, PFL Chang, A Ahroni, KT Cheah, WR Woodson, RA Bressan, AA Watad, PM Hasegawa and AA Vainstein*. 1995. Transformation of carnation by microprojectile bombardment. Scientia Horticulturae 64: 177-185. (SCI journal) (SCI: Horticulture 9/32, IF=1.396).

  Yeh CH, KW Yeh, SH Wu, PFL Chang, YM Chen and CY Lin. 1995. A recombinant rice 16.9-kD heat shock protein can provide thermoprotection in vitro. Plant Cell Physiology 36: 1341-1348. (SCI journal) (SCI: Plant sciences 20/ 197, IF=4.134) (NSC 81-0211-B002-13 & NSC 82-0211-B002-241).

  Lee YL, PFL Chang, KW Yeh, TL Jinn, CCS Kung, WC Lin, YM Chen and CY Lin. 1995. Cloning and characterization of a cDNA encoding an 18.0-kD class-I low-molecular-weight heat-shock-protein from rice. Gene 165: 223-227. (SCI journal) (SCI: Genetics & heredity 99/ 161, IF=2.196) (NSC 82-0211-B002-317 & NSC 83-0211-B002-232).

  Xu Y, PFL Chang, D Liu, ML Narasimhan, KG Raghothama, PM Hasegawa and RA Bressan. 1994. Plant defense genes are synergistically induced by ethylene and methyl jasmonate. Plant Cell 6: 1077-1085. (SCI journal) (SCI: Plant sciences 4/ 197, IF=9.251).

  Chen Z, H Fu, D Liu, PFL Chang, ML Narasimhan, R Ferl, PM Hasegawa and RA Bressan. 1994. NaCl-regulated plant gene encoding a brain protein homologue that activates ADP-ribosyl-transferase and inhibits protein kinase C. Plant Journal 6: 729-740. (SCI journal) (SCI: Plant sciences 7/ 197, IF=6.582).

  Chang PFL, ML Narasimhan, PM Hasegawa and RA Bressan. 1993. Quantitative mRNA-PCR for expression analysis of low-abundance transcripts. Plant Molecular Biology Reporter 11: 237-248. (SCI journal) (SCI: Plant sciences 12/ 197, IF=5.319).

  Chang PFL. 1990. Hypothesis of Auxin-induced Cell Elongation in Legume Plants. Scientific Agriculture 38 (1-2): 22-27. (In Chinese).

張碧芳。1990。生長素 (AUXIN) 誘導豆科植物細胞伸長的假說。科學農業 38 (1-2): 22-27

 

(B) 研討會論文

          Pi-Fang Linda Chang, Wen-Hsin Chung, Yao-Tung Lin and Jenn-Wen Huang (2015, Dec). Applications and Implications of Nanotechnology in Agriculture.International Symposium for Agricultural Biotchnology, Kasetsart University,Kamphaeng Saen, Thailand.本人為第一作者、通訊作者. (16-20 Dec.,2015)


          Pi-Fang Linda Chang, Yong-Wen Liu, Jiang-Jhen Lin, and Jenn-Wen Huang (2015, Sep). Effects of Nanomaterials on Fusarium oxysporum f. sp. niveum and Vegetable Seeding Growth . 11th International Symposium on Biocatalysis and Agricultural Biotechnology, Banff, Alberta, Canada.本人為第一作者、通訊作者.(13-16 Sep.,2015)


          Pi-Fang Linda Chang, Pei-Hsuan Chan, Ying-Hong Lin, Chien-Chih Chen, Kan-Shu Chen, Jenn-Wen Huang (2015, Aug). Disease severity and colonization of Fusarium oxysporum f. sp. niveum transformants on watermelon. 2015 APS Annual Meeting, Pasadena, California, U.S.A..本人為第一作者、通訊作者.(1-5 Aug.,2015)


          Y. J. Lin, J. C. Hsu, Y. H. Chiu, L. L. Hong, T. D. Chang, P. F. L. Chang, Y. H. LIN. (2015, Aug). Development of TaqMan probebased insulated isothermal PCR (iiPCR) for detection of Fusarium oxysporum f. sp. cubense race 4.. 2015 APS Annual Meeting, Pasadena, California, U.S.A..(1-5 Aug., 2015)


          Chang P.-F. L., Lin Y.-H., Chang T.-H., Shih S.-L., Chen K.-S., Huang J.-W. (2015, May). Applications of transcriptome sequencing technology for analysis of molecular mechanisms of Fusarium wilt resistance . 13th European of Fusarium Saminar, Martina Franca, Italy.本人為第一作者、通訊作者. (10-14 May.,2015)


          Chan PH*, YH Lin, JW Huang, PFL Chang. 2013. Effects of dimethyl disulfide produced by Bacillus mycoides on Fusarium oxysporum. The Congress of Mycological Society of Republic of China. Taichung, Taiwan. Abstract book, p. 33-34. (December 7, 2013)(*presenter)

 

          Chang PFL*, YH Lin, CC Chen, YH Lin, YS Lin. Adjustment of Crops to Biological Stresses. The Workshop on Climate-Smart Agricultural Production. Tatsuen Hsiang, Changhua, Taiwan (October, 23, 2013) (*presenter)

張碧芳*、林盈宏、陳建志、林奕宏、林昀聖。 2013。作物對生物性逆境的調適。氣候智能型農作生產研討會。台灣、彰化。(October 23, 2013) (*presenter)

 

          Lin YH*, TH Chang, KS Chen, JW Huang, and PFL Chang. 2013. RNA-Seq analysis of Fusarium wilt-resistant and -susceptible watermelons. Advances in Molecular Plant-Microbe Interactions. Taipei, Taiwan. Abstract Book, p. 29 (D-7). (October 3-4, 2013) (*presenter)

 

          Chang PFL*, YH Lin, TH Chang, KS Chen, JW Huang. 2013. Analysis of the resistance to Fusarium wilt in a Fusarium wilt-resistant watermelon line. 2013 The Cross-Strait Forum of the Young Scientists of Plant Pathology. Beijing, China Abstract Book, p. 11. (September 2-3, 2013) (*presenter)

 

          Huang YJ*, JW Huang, JJ Lin, PFL Chang, WH Chung. 2013. Evaluation of nano-materials on improving efficacy of inhibition to fungicide-resistant isolates. 10th International Congress of Plant Pathology. Beijing, China. (August 25-30, 2013) (*presenter)

          Chang PFL*, TH Chang, YH Lin, KS Chen, JW Huang, and SC Hsiao. 2013. Comparative analysis of resistance in Fusarium wilt-resistant and-susceptible watermelons: reinforcement of the structure barrier. 12th European Fusarium Seminar. Bordeaux, France. (May 13-16, 2013) (*presenter)

          Liu YW*, YH Lin, JJ Lin, JW Huang, PFL Chang. 2013. Effects of AgNP/NSP nanohybrids on Fusarium oxysporum f.sp. niveum. The Annual Meeting of Taiwan Phytopathological Society, Taichung, Taiwan. (April 26-27, 2013) (*presenter)

          Huang YJ*, JW Huang, JJ Lin, PFL Chnag, WH Chung. 2013. Study of nanomaterials on controlling phytopathogens. The 2012 Annual Meeting of Taiwan Phytopathological Society, Taichung, Taiwan. (April 26-27, 2013).

黃盈潔*黃振文、林江珍、張碧芳、鍾文鑫。2013。奈米資材於防治植物病原菌之相關研究。中華民國植物病理學年會。台中,台灣。(April 26-27, 2013) (*presenter)

          Dita MA, C Waalwijk, P Mutua, A Daly, PFL Chang, BM Corcolon, L Paiva, M Souza, J Kema GH. 2013. Detecting Fusarium oxysporum f. sp. cubense tropical race 4 in soil and symptomless banana tissues. Acta Horticulturae (ISHS) 986: 127-130. (25 April 2013)

      Dita MA*, C. Waalwijk, A. Daly, PFL Chang, BM Corcolon, and GHJ. Kema. 2012. An early diagnostic of Fusarium wilt of banana; recent advances and the implication for disease management. International Banana Symposium. Kaohsiung, Taiwan. (November 19-22, 2012) (*presenter)

      Lin YH, CC Su, CP Chao, JW Huang, and PFL Chang*. 2012. An efficient and reliable molecular method for detection of Fusarium oxysporum f. sp. cubense race 4 using real time PCR. International Banana Symposium. Kaohsiung, Taiwan. (November 19-22, 2012) (*presenter)

      Huang JW*, MK Chuang, PFL Chang, and YH Lin. 2012. Diagnosis of crop Fusarial wilt diseases and case analysis. Symposiums for identification and diagnosis of plant pests with case report analysis. Taipei, Taiwan. (November 16, 2012) (*presenter)

      Lin YH*, KS Chen, JW Huang, and PFL Chang. 2012. The molecular detection methods of Fusarium oxysporum f. sp. niveum. 8th International Symposium on Biocatalysis and Agricultural Biotechnology. California, USA. (October28-31, 2012) (*presenter)

      Chang PFL*, YH Lin, CC Su, CP Chao, and JW Huang. 2012. A molecular diagnosis method for quantification and detection of Fusarium oxysporum f. sp. cubense race 4. 8th International Symposium on Biocatalysis and Agricultural Biotechnology. California, USA. (October 28-31, 2012) (*presenter)

      Tong JJ*, YH Lin, PFL Chang, and JW Huang. 2012. Assessment skill for tomato plants resistant to Fusarium wilt disease induced by biocontrol agents. The 2011 Annual Meeting of Taiwan Phytopathological Society. Taipei, Taiwan (April 13-14, 2012) (*presenter)

      Chan PH*, YH Lin, JW Huang, and PFL Chang. 2012. Evaluation for the efficacy of Bacillus mycoides on controlling Fusarium wilt of watermelon. The 2011 Annual Meeting of Taiwan Phytopathological Society. Taipei, Taiwan (April 13-14, 2012) (*presenter)

      Chang TH*, YH Lin, KS Chen, JW Huang, and PFL Chang. 2012. Comparing the cell wall components of vascular tissues in Fusarium wilt-resistant and -susceptible watermelon lines. The 2011 Annual Meeting of Taiwan Phytopathological Society. Taipei, Taiwan (April 13-14, 2012) (*presenter)

      Lin YH*, CC Hsu, JY Chang, KS Chen, JW Huang, and PFL Chang. 2012. Studies on watermelon Fusarium wilt: the resistance mechanism, molecular markers for resistance, and molecular detection of its pathogen. The 2011 Annual Meeting of Taiwan Phytopathological Society, Taipei, Taiwan (April 13-14, 2012) Abstract Book, p. 16-17.

      Lin YH, KS Chen, JW Huang, and PFL Chang. 2012. Development of the molecular detection methods for Fusarium oxysporum f. sp. niveum. The 1st Biotechnology World Congress, Dubai, UAE (February 14-15, 2012) (*presenter)

      Liu YW*, YH Lin, JJ Lin, JW Huang, and PFL Chang. 2011. Effects of AgNP/NSP nanohybrids on Fusarium oxysporum f. sp. niveum and watermelon seedlings growth. The 2nd Symposium on the Applications and the Implications of Nanotechnology in Environmental and Agricultural Systems, Nantou, Taiwan (December 30-31, 2011) (*presenter)

      Chang TH, CC Hsu, YH Lin, KS Chen, JW Huang, and PFL Chang*. 2011. The relationship between phenylpropanoid pathway and watermelon resistance to Fusarium wilt. The 2nd Cross-Strait Symposium on Plant Science & Agricultural Biotechnology, Guangzhou, China (November 22-26, 2011) (*presenter)

      Chen CC*, YH Lin, YS Lin, YH Lin, and PFL Chang. 2011. Expression of rice small heat shock proteins under stress and their protective functions in Escherichia coli. The 9th International Symposium of Rice Functional Genomics, Taipei, Taiwan (November 7-9, 2011) (*presenter)

      Dita MA*, C Waalwijk, P Mutua, A Daly, PFL Chang, BM Corcolon, L Paiva, M Souza, and GHJ Kema. 2011. Detecting Fusarium oxysporum f. sp. cubense tropical race 4 in soil and symptomless banana tissues. The ISHS/ProMusa Symposium: "Bananas and Plantains: Toward Sustainable Global Production and Improved Uses". Salvador, Bahia, Brazil (October 10-14, 2011) (*presenter)

      Lin YH, JY Chang, TH Chang, KS Chen, JW Huang, and PFL Chang*. 2011. Development of the molecular methods for rapid detection of Fusarium oxysporum f. sp. niveum in Taiwan. Symposium on Diagnosis and Identification of Crop Pests in Taiwan: Present and Future Outlooks, Taipei, Taiwan (September 8-9, 2011) (*presenter)

      Lin YH, PJ Lai, TH Chang, YL Wan, JW Huang, and PFL Chang*. 2011. Pathological and molecular race determinations of Fusarium oxysporum f. sp. lactucae from Taiwan. 2011 American Phytopathological Society (APS)-International Plant Protection Congress (IPPC) Joint Meeting. Honolulu, Hawaii, U.S.A. (August 6-10, 2011) (*presenter)

      Chen YR, YH Lin, TC Wang, ZM Sheu, Y Chen, KC Kuo, and PFL Chang*. 2011. Preliminary studies on the resistance of pepper accessions AVRDC PBC81 (Capsicum baccatum) and PBC932 (Ca. chinense) to Colletotrichum acutatum. The 10th Cross-Strait Symposium on Mycology & the 3rd Conference on Edible and Medicinal Fungi. Wuhan, China (July 15-17, 2011). Abstract Book, p. 286-287. (*presenter)

      Lin YH, PJ Lai, TH Chang, YL Wan, JH Huang, JW Huang, and PFL Chang*. 2011. Development of molecular identification for races of Fusarium oxysporum f. sp. lactucae from Taiwan. The 2011 Cross-Strait Symposium of Plant Pathology, Taipei, Taiwan (May 25-26, 2011).

      Liu YW, HW Chen, JJ Lin, JW Huang, and PFL Chang*. 2011. The inhibition effect of AgNP/NSP nano hybrids on plant wilt pathogen Fursarium oxsporum. 2011 Nanotechnology Industry platform conference of National Chung Hsing University. Nantou County, Taiwan (April 22-23, 2011). (The ATU plan, Ministry of Education, Taiwan, R. O. C., and National Chung Hsing University, Taiwan, R.O.C.). (*presenter)

劉詠汶、陳和緯、林江珍、黃振文、張碧芳*2011奈米矽片銀對作物萎凋病菌 Fusarium oxysporum 之抑制效果。2011國立中興大學奈米科技產業鏈結論壇暨成果發表會,南投縣惠蓀林場,台灣(2011422-23日)。

      Chen YR, CH Tsao, CC Yen, TC Wang, ZM Sheu, Y Chen, KC Kuo, and PFL Chang*. 2010. Preliminary studies on the resistance mechanism of ‘PBC932’, a resistant Capsicum chinense germplasm accession to Colletotrichum acutatum. The 28th International Congress of Horticulture (IHC Lisboa 2010), Lisbon, Spain (August 22-27, 2010). (93AS-4.1.2- BQ-B1, 94AS-5.1.4-BQ-B1) (*presenter)

      Chang PFL*, KS Chen, YH Lin, and JW Huang. 2010Development of a molecular method for rapid differentiation of watermelon lines resistant to Fusarium oxysporum f. sp. niveum. 2010 Cross-Strait Symposium on Breeding Research of Watermelon and Melon. Kaohsiung City, Taiwan. (April 28-29, 2010). (90AS-2.1.2-BQ-B7, NSC 91-2313-B-005-094, NSC 92-2313-B-005-054, NSC 93-2313-B-005-080) (*presenter)

張碧芳*、陳甘澍、林盈宏、黃振文。2010。開發西瓜抗蔓割病品系之快速分子檢測技術。兩岸西甜瓜育種研究交流會,高雄市,台灣(2010428-29日)。

      Chen Y, RC Su, HW Chen*, and PFL Chang. 2010. Effect of TiO2 nanoparticals on plant calli and suspension cells. The First Symposium on the Applications and the Implications of Nanotechnology in Agricultural Systems. Taichung City, Taiwan (March 28, 2010). (The ATU plan, Ministry of Education, Taiwan, R. O. C., and National Chung Hsing University, Taiwan, R.O.C.) (*presenter)

      Lai PJ*, YL Wan, JH Huang, YH Lin, JW Huang, and PFL Chang. 2010. Molecular identification of Fusarium oxysporum f. sp. lactucae races in Taiwan. The 2009 Annual Meeting of Taiwan Phytopathological Society, Taipei, Taiwan (March 5-6, 2010). Abstract Book, p. 56 (Abstract No. SC05). Plant Pathology Bulletin 19(1). (95AS-13.3.1-BQ-B1(6), 96AS-14.3.1-BQ-B1(6), 97AS-14.3.1-BQ-B1(11), the ATU plan, Ministry of Education, Taiwan, R. O. C., and National Chung Hsing University, Taiwan, R.O.C.) (*presenter)

      PFL Chang, YH Lin, JY Chang, YL Wan, PJ Lai, KS Chen, CP Chao, and JW Huang. 2009. Development of molecular detection methods for Fusarium oxysporum pathogens infecting banana, lettuce, and watermelon. Asian Mycological Congress 2009 & The 11th International Marine and Freshwater Mycology Symposium. Taichung City, Taiwan (November 15-19, 2009). Abstract Book O-24. (89ST-6.2-BQ-65(06), 90AS-6.3.1-BQ-B2(6), 91AS-7.3.1-BQ-B2(3), 93AS-1.9.2-BQ-B1(1), 94AS-13.3.2-BQ-B1(6), 95AS-13.3.1-BQ-B1(6), 96AS-4.1.2-IC-I1(2), 96AS-14.3.1-BQ-B1(6), 97AS-4.1.2-IC-I1(6), 97AS-14.3.1-BQ-B1(11), 98AS-4.1.1-IC-I1(1), and the ATU plan, Ministry of Education, Taiwan, R. O. C.)

      PFL Chang, YH Lin, JY Chang, CP Chao, and JW Huang. 2009. Development of the molecular diagnosis method for detection of Fusarium oxysporum f. sp. cubense race 4 in Taiwan. In: Proceedings of 2009 Cross-Strait Symposium of Plant Pathology (November 3-8, 2009). Hangzhou, China. Assembly Manual, pp. 23. (89ST-6.2-BQ-65(06), 90AS-6.3.1-BQ-B2(6), 91AS-7.3.1-BQ-B2(3), 93AS-1.9.2-BQ-B1(1), 96AS-4.1.2-IC-I1(2), 97AS-4.1.2-IC-I1(6), 98AS-4.1.1-IC-I1(1), and the ATU plan, Ministry of Education, Taiwan, R. O. C.)

      PFL Chang, YH Lin, JY Chang, CP Chao, and JW Huang. 2009. A molecular detection method specific to Fusarium oxysporum f. sp. cubense race 4 in Taiwan. ISHS/ProMusa Symposium "Global Perspectives on Asian Challenges". Guangzhou, China (September 14-18, 2009). Program Book p. 117. (89ST-6.2-BQ-65(06), 90AS-6.3.1-BQ-B2(6), 91AS-7.3.1-BQ-B2(3), 93AS-1.9.2-BQ-B1(1), 96AS-4.1.2-IC-I1(2), 97AS-4.1.2-IC-I1(6), 98AS-4.1.1-IC-I1(1), and the ATU plan, Ministry of Education, Taiwan, R. O. C.)

      YH Lin, PFL Chang, JY Chang, KS Chen, and JW Huang. 2009. Molecular detection of Fusarium oxysporum f. sp. niveum, the causing agent of watermelon Fusarium wilt disease. 2009 American Phytopathological Society (APS) Annual Meeting. Portland, Oregon, U.S.A. (August 1-5, 2009). Poster Number is P-344. (93AS-1.9.2-BQ-B1 (1), 94AS-13.3.2-BQ-B1(6), and the ATU plan, Ministry of Education, Taiwan, R. O. C.)

      Tsao CH*, YR Chen, ZM Sheu, TC Wang, KC Kuo, and PFL Chang. 2010. Identification of defense-related genes in Capsicum chinense responding to Colletotrichum acutatum inoculation. The 2009 Annual Meeting of Taiwan Phytopathological Society, Taipei, Taiwan (March 5-6, 2010). Abstract Book, p. 59-60 (Abstract No. SC10). Plant Pathology Bulletin 19(1). (95AS-13.1.2-BQ-B3(11), 96AS-1.1.1-BQ-B1(2), 97AS-14.2.3-BQ-B5(6)) (*presenter)

      Chang PFL*, YH Lin, JY Chang, KS Chen, and JW Huang. 2009. Molecular detection of Fusarium oxysporum f. sp. niveum, the causing agent of watermelon Fusarium wilt disease. 2009 American Phytopathological Society (APS) Annual Meeting, Portland, Oregon, U.S.A. (August 1-5, 2009). Poster Number is P-344. (93AS-1.9.2-BQ-B1(1), 94AS-13.3.2-BQ-B1(6), and the ATU plan, Ministry of Education, Taiwan, R. O. C.) (*presenter)

      Chang PFL*, YH Lin, JY Chang, CP Chao, and JW Huang. 2009. A molecular detection method specific to Fusarium oxysporum f. sp. cubense race 4 in Taiwan. ISHS/ProMusa Symposium "Global Perspectives on Asian Challenges". Guangzhou, China (September 14-18, 2009). Program Book p. 117. (89ST-6.2-BQ-65(06), 90AS-6.3.1-BQ-B2(6), 91AS-7.3.1-BQ-B2(3), 93AS-1.9.2-BQ-B1(1), 96AS-4.1.2-IC-I1(2), 97AS-4.1.2-IC-I1(6), 98AS-4.1.1-IC-I1(1), and the ATU plan, Ministry of Education, Taiwan, R. O. C.) (*presenter)

      Chang PFL*, YH Lin, JY Chang, YL Wan, PJ Lai, KS Chen, CP Chao, and JW Huang. 2009. Development of molecular detection methods for Fusarium oxysporum pathogens infecting banana, lettuce, and watermelon. Asian Mycological Congress 2009 & The 11th International Marine and Freshwater Mycology Symposium. Taichung City, Taiwan (November 15-19, 2009). Abstract Book O-24. (89ST-6.2-BQ-65(06), 90AS-6.3.1-BQ-B2(6), 91AS-7.3.1-BQ-B2(3), 93AS-1.9.2-BQ-B1(1), 94AS-13.3.2-BQ-B1(6), 95AS-13.3.1-BQ-B1(6), 96AS-4.1.2-IC-I1(2), 96AS-14.3.1-BQ-B1(6), 97AS-4.1.2-IC-I1(6), 97AS-14.3.1-BQ-B1(11), 98AS-4.1.1-IC-I1(1), and the ATU plan, Ministry of Education, Taiwan, R. O. C.) (*presenter)

      Chang PFL*, YH Lin, JY Chang, CP Chao, and JW Huang. 2009. Development of the molecular diagnosis method for detection of Fusarium oxysporum f. sp. cubense race 4 in Taiwan. In: Proceedings of 2009 Cross-Strait Symposium of Plant Pathology (November 3-8, 2009). Hangzhou, China. Assembly Manual, pp. 23. (89ST-6.2-BQ-65(06), 90AS-6.3.1-BQ-B2(6), 91AS-7.3.1-BQ-B2(3), 93AS-1.9.2-BQ-B1(1), 96AS-4.1.2-IC-I1(2), 97AS-4.1.2-IC-I1(6), 98AS-4.1.1-IC-I1(1), and the ATU plan, Ministry of Education, Taiwan, R. O. C.) (*presenter)

張碧芳*、林盈宏、張景宜、趙治平、黃振文。2009。台灣香蕉黃葉病菌生理小種第四型之分子檢測技術研發。2009年海峽兩岸植物病理學術研討會,杭州,中國(2009113日至8日)。大會手冊第23頁。

      Lin YS*, and PFL Chang. 2009. Expression profile of rice small heat shock proteins under UV-C treatments. The Botanical Society Annual Meeting 2009, Taichung City, Taiwan (November 28-29). (*presenter)

      Chang TH*, YH Lin, PFL Chang, KS Chen, and JW Huang. 2009. The activity of phenylalanine ammonia lyase and the amount of soluble phenolic compounds related to the resistance of watermelon plants to Fusarium wilt. The 2008 Annual Meeting of Taiwan Phytopathological Society, Taichung, Taiwan. (February 5, 2009). Abstract Book, p. 50-51 (Abstract No. SC08). Plant Pathology Bulletin 18(1). (*presenter)

  Wan YL*, PFL Chang, YH Lin, and JW Huang. 2009. Effects of cultural variability on growth rate, condition, and virulence in Fusarium oxysporum f. sp. niveum. The 2008 Annual Meeting of Taiwan Phytopathological Society, Taichung, Taiwan. (February 5, 2009). Abstract Book, p. 51 (Abstract No. SC09). Plant Pathology Bulletin 18(1). (*presenter)

      Chen HW*, JW Huang, and PFL Chang. 2008. Preliminary studies on Bacillus mycoides affecting tomato defense-related genes expression and promoting plant growth. The 2008 Joint Annual Meeting of Plant Protection Society of the Republic of China and Taiwan Phytopathological Society, Taichung, Taiwan. (January 1, 2008). Plant Pathology Bulletin 17(1) 96. (*presenter).

      Chen YR*, D Wang, and PFL Chang. 2008. Effects of hot pepper fruit contents on Colletotrichum acutatum causing anthracnose. The 2008 Joint Annual Meeting of Plant Protection Society of the Republic of China and Taiwan Phytopathological Society, Taichung, Taiwan. (January 1, 2008). Plant Pathology Bulletin 17(1) 77. (*presenter).

      Chang PFL*, YH Lin, JY Chang, ET Liu, CP Chao, and JW Huang. 2008. Development of a molecular marker for specific detection of Fusarium oxysporum f. sp. cubense, a causing agent of banana wilts disease. 2008 American Phytopathological Society (APS) Centennial Meeting, Minneapolis, Minnesota, U.S.A. (July 26-30, 2008). Poster No: P-137. (*presenter). (89ST-6.2-BQ-65(06), 91AS-7.3.1-BQ-B2(3), 93AS-1.9.2-BQ-B1, 96AS-4.1.2-IC-I1(2), and 97AS-4.1.2-IC-I1(6))

      Hsu CC, PFLChang*, YH Lin, KS Chen, JW Huang, and TD Liou. 2007. Mechanisms involved in resistance to watermelon Fusarium wilt. International Conference on Plant Vascular Biology 2007. Academia Sinica, Taipei, Taiwan. (May 7-11, 2007).Program and Abstract Book p. 81-82 (*presenter).

      Wang CL, PFL Chang, and PP Ueng. 2007. Genetic diversity and identification of cereal leaf blotch pathogens, Phaeosphaeria species. In: Proceedings of 2007 Cross-Strait Symposium on Progresses of Modern Plant Pathology, CY Chen, PFL Chang, and JW Huang eds, Taiwan Phytopathological Society, Taichung, Taiwan., R.O.C., (March 19-23, 2007). pp. 79-98.  (*presenter).

      Chang PFL*, YH Lin and JY Chang. 2007. Development of Molecular Detection Methods for Some Fusarium oxysporum Pathogens Infecting Watermelon, Banana, and Tomato. 8th Cross-Strait Symposium on Mycology. Changchun, Jilin, China. (August 15-21, 2007). P. 360. (*presenter)

      Lin WW-C, and PFL Chang*. 2007. Approaches for acquired tolerance to and abiotic stress of economically important crops. 2007 International Symposium on Biocatalysis and Biotechnology. National Chung Hsing University, Taichung, Taiwan. (November 28-30, 2007). Program and Abstract Book p. 58 (*co-presenter).

      Malkus A, PFL Chang, E Arseniuk, and PP Ueng*. 2006. The RNA polymerase II gene (RPB2) in Phaeosphaeria nodorum. The 2006 Annual Meeting of American Phytopathological Society, Quebec City, Canada. (July 29-August 2, 2006). (*presenter)

      Chang PFL*, CC Hsu, YH Lin, JW Huang, KS Chen, and TD Liou. 2006. Resistance mechanism of watermelon to Fusarium wilt: a case study of resistant JSB line in Taiwan. Plant Biology 2006, Final Program and Abstract Supplement, p. 183-184. Boston, Massachusetts, USA. (August 5-9, 2006). (*presenter). (NSC 91-2313-B-005-094; NSC 92-2313-B-005-054; NSC 93-2313-B-005-080)

      Chen Y*, JF Hong, and PFL Chang. 2006. An improved method for assessing disease resistance and stress responses of different crop varieties at seedling stage. 27th International Horticultural Congress (IHC 2006), Seoul, Korea. (August 13-19, 2006).Presenting Number: S13-P-50. (*presenter).

      Chang PFL*, and JW Huang. 2006. Introduction of fruit crops diseases and their controls in Taiwan. Plant Protection Forum and Academic Reports. In: The 2006 China Plant Protection Information Exchanges and Agrochemicals and Agro-machineries Trade Exhibition. Xia-Men, Fujian, China. (November 23, 2006)(*presenter).

      Lin YH, PR Cheng, CC Hsu, and PFL Chang*. 2006. Using PCR technique for rapid detection of Fusarium wilt pathogen, Fusarium oxysporum f. sp. niveum, in watermelon seedlings. The 2006 Poster Contest for Plant Disease Control and its Educational Publicity--Diagnosis and Identification. Taiwan Phytopathological Society, Taichung, Taiwan. (December 16, 2006) (*presenter).

      Chen YR*, PFL Chang, and TC Wang. 2006. Preliminary studies on the resistance of hot pepper accessions PBC81 (Capsicum baccatum) and PBC932 (C. chinense) to anthracnose caused by Colletotrichum acutatum. The 2006 Joint Annual Meeting of Plant Protection Society of the Republic of China and Taiwan Phytopathological Society, Jia-Yi, Taiwan. (December 16, 2006). Plant Pathology Bulletin 15(4) 295-296. (*presenter).

      Lin YH*, and PFL Chang. 2006. Accumulation of heat shock proteins (HSPs) during seed maturation and drought stress in rice. The 2006 Joint Annual Meeting of Plant Protection Society of the Republic of China and Taiwan Phytopathological Society, Jia-Yi, Taiwan. (December 16, 2006).  Plant Protection Bulletin 15(4) 311. (*presenter).

      Chang PFL*, WK Huang, TL Jinn, CW Wang, PL Lin, and HM Chang. 2005. The class II small heat shock gene of rice (Oryza sativa L.), Oshsp18.0-CII, is induced by heat stress, mechanical injury, and also confers tolerance to heat and UV stresses when overexpressing it in Escherichia coli. Plant Biology 2005, Seattle, WA, USA. (July 16-20, 2005). Plant Biology 2005 Final Program and Abstract Supplement p. 170 (Poster No. 384). (*presenter). (NSC88-2311-B-005-042, NSC88-2313-B-005-022 and NSC89-2313-B-005-159)

      Chang PFL, CC Hsu*, YH Lin, JW Huang, KS Chen, and TD Liou. 2005. Molecular markers related to Fusarium wilt resistance and the expression analysis of an acidic class III chitinase gene in watermelon plants. Plant Biology 2005, Seattle, WA, USA. (July 16-20, 2005). Plant Biology 2005 Final Program and Abstract Supplement p. 130-131 (Poster No. 178) (*presenter). (90AS-2.1.2-BQ-B7; NSC 91-2313-B-005-094; NSC 92-2313-B-005-054; NSC 93-2313-B-005-080)

      Chang PFL*, and YR Chen. 2005. Expression of defense-related genes in chili pepper induced by salicylic acid and Colletotrichum infection. The 2005 Annual Meeting of American Phytopathological Society, Austin, Texas, USA. (July 30-August 3, 2005).Phytopathology S18. (*presenter). (93AS-4.1.2-BQ-B1(4); 94AS-5.1.4-BQ-B1(4))

      Lin PL*, YH Lin, and PFL Chang. 2005. The accumulation of different HSPs in rice seedlings under heat stress and during seed development and maturation. The Botanical Society Annual Meeting 2005, Ping-Tung, Taiwan. Abstract Book 25-26. (*presenter).

      Chen YR*, and PFL Chang. 2005. Isolation and expression of defense-related genes under salicylic acid treatment and inoculation of Colletotrichum acutatum in chili pepper. The 2006 Annual Meeting of Taiwan Phytopathological Society, Taipei, Taiwan. (December 16-17, 2005). Plant Pathology Bulletin 14(4) 304-305. (*presenter). (93AS-4.1.2-BQ-B1(4); 94AS-5.1.4-BQ-B1(4))

      Hsu CC*, YH Lin, KS Chen, and PFL Chang. 2004. Distribution of Fusarium oxysporum f. sp. niveum and expression of the acidic class III chitinase gene in resistant and susceptible watermelon plants. The 2003 Annual Meeting of Taiwan Phytopathological Society, Taipei. (December 4, 2004). Plant Pathology Bulletin 13(4) 357-358. (*presenter). (NSC 93-2313-B-005-080)

      Lin PL, and PFL Chang. 2004. The difference in accumulation of the class I sHSP from rice plants grown under oxidative stresses, Rhizoctonia solani R96 infection, and during their developmental processes. The 2004 Annual Meeting of Taiwan Phytopathological Society, Taipei, Taiwan. (December 4, 2004).  Plant Pathology Bulletin 13(4) 358. (*presenter).

      Ding JW*, JW Huang, and Chang PFL. 2004. Morphology and Phylogeny of Fusarium species in section Gibbosum from Taiwan. The 2004 Annual Meeting of Taiwan Fungal Society, Taichung, Taiwan. (January 7, 2004). Abstract Book 12-13. (*presenter).

      Lin YH*, KS Chen, JW Huang, and PFL Chang. 2003. Screening of DNA markers related to Fusarium wilt resistance in watermelon based on RAPD technique. The Botanical Society Annual Meeting 2003, Taichung, Taiwan. (*presenter). (90AS-2.1.2-BQ-B7; NSC 91-2313-B-005-094; NSC 92-2313-B-005-054)

      Hsu CC*, and PFL Chang. 2003. Isolation and characterization of a cDNA clone encoding a putative acidic class III chitinase from watermelon. The Botanical Society Annual Meeting 2003, Taichung, Taiwan. (*presenter). (NSC 92-2313-B-005-054)

      Huang WK*, YR Chen, and PFL Chang. 2003. Induced thermotolerance of Escherichia coli by a rice class II low-molecular-mass heat-shock protein, Oshsp18.0-II. The Botanical Society Annual Meeting 2003, Taichung, Taiwan. (*presenter).

      Hong* CF, JW Huang, and PFL Chang. 2003. Identification for the casual agent of caspia (Limonium bellidifolium) anthracnose. The 2003 Annual Meeting of Taiwan Phytopathological Society, Taichung, Taiwan. (December 13-14, 2003) Plant Pathology Bulletin 12(4) 276. (*presenter).

      Chang PFL, JY Chang*, EZ Liu, YR Chen, and JW Huang. 2003. Detection of Fusarium oxysporum f. sp. cubense based on RAPD and PCR analysis. The 2003 Annual Meeting of Taiwan Phytopathological Society, Taichung, Taiwan. (December 13-14, 2003). Plant Pathology Bulletin 12(4) 277. (*presenter). (91AS-7.3.1-BQ-B2(3))

      Liu ET*, and PFL Chang. 2002. Specific detection of Fusarium oxysporum f. sp. cubense using random amplified polymorphic DNA analysis and polymerase chain reaction. The 2002 Annual Meeting of Botanical Society of Republic of China, Taichung, Taiwan. Abstract. (*presenter). (89AS-6.2-BQ-65(06), 90AS-6.3.1-BQ-B2(6), 91AS-7.3.1-BQ-B2(3))

      Chang HM*, and PFL Chang. 2002. Differences in the expression of low-molecular-mass heat shock genes and pathogenesis-related genes induced by biotic and abiotic stresses in rice. The 2002 Annual Meeting of Botanical Society of Republic of China, Taichung, Taiwan. Abstract. (*presenter). (NSC88-2311-B-005-042, NSC89-2313-B-005-159).

      Lin YH*, CC Hsu, KS Chen, JW Huang, and PFL Chang. 2002. Relationships of the pathogenesis-related gene expression and Fusarium wilt resistance in watermelon. The 2002 Annual Meeting of Phytopathological Society of the Republic of China, Taichung, Taiwan. Plant Pathology Bulletin 11(4) 247. (*presenter). (NSC91-2313-B-005-094)

      Chen KS*, PFL Chang, JW Huang, WL Lee, and TD Liou 2001. Molecular Markers Linked to Fusarium Wilt Resistance in Watermelon Based on Random Amplified Polymorphic DNA Analysis. The Second International Symposium on Biotechnology of Tropical & Subtropical Species. Taipei, Taiwan, R.O.C. (November 5-9, 2001). (*presenter).

      Chang HM*, and PFL Chang. 2001. Differential induction of low-molecular-mass heat shock genes and pathogenesis-related genes in rice by biotic and abiotic stresses. The 2001 Joint Annual Meeting of Phytopathological Society of the Republic of China and Plant Protection Society of the Republic of China, Ping-Tung, Taiwan. (December 15-16, 2001). Plant Pathology Bulletin 10(4) 217. (*presenter).

      Chang PFL*, HS Chang, KW Yeh, YM Chen, and CY Lin 2000. A putative class II low-molecular-mass heat shock protein gene of rice (Oryza sativa L.) is induced by mechanical injury. Abstract S31-15. The Sixth International Congress of Plant Molecular Biology, Quebec, Canada, (July 14-18, 2000). (*presenter). (NSC84-2311-B-002-007 B01, NSC85-2311-B-002-010 B13, NSC84-2311-C002-023, NSC85-2811-B002-006 & NSC88-2311-B-005-042).

      Chang PFL*, CY Huang, FC Chang, WC Lin, TS Tseng, YH Lin, and CY Lin 2000. Characterization of a gene, Oshsp16.9C, encoding a 16.9 kDa class I low-molecular-mass heat shock protein in rice. Plant Biology 2000. (July 15-19, 2000). Abstract 438. (*presenter). (NSC 84-2311-B-002-007-B01 & NSC 85-2311-B-002-010-B13).

      Chang PFL*, HS Chang, KW Yeh, YM Chen, and CY Lin 2000. A putative class II low-molecular-mass heat shock protein gene of rice (Oryza sativa L.) is induced by heat stress and mechanical injury. Abstract P59. The Second Brook-Top Symposium—Post Genomics: Gene Expression and Function. Nan-Tao, Taiwan, 2000. (*presenter). (NSC84-2311-B-002-007 B01, NSC85-2311-B-002-010 B13, NSC84-2311-C002-023, NSC85-2811-B002-006 & NSC88-2311-B-005-042). (*presenter).

      Chang PFL*, CY Huang, FC Chang, TS Tseng, YH Lin, and CY Lin 2000. Isolation and characterization of another gene, Oshsp16.9C, encoding a rice 16.9 kDa class I low-molecular-mass heat shock protein. Abstract P60. The Second Brook-Top Symposium—Post Genomics: Gene Expression and Function. Nan-Tao, Taiwan, 2000. (*presenter). (NSC 84-2311-B-002-007-B01 & NSC 85-2311-B-002-010-B13).

      Liu, EZ*, and PFL Chang and JW Huang. 2000. Detection of Fusarium oxysporum f. sp. cubense using random polymorphic DNA (RAPD) analysis. The 2000 Joint Annual Meeting of Phytopathological Society of the Republic of China and Plant Protection Society of the Republic of China, Taipei, Taiwan. Plant Pathology Bulletin 9(4) 193-194. (*presenter). (89ST-6.2-BQ-65(06))

      Lin WC, PFL Chang, and CY Lin* 1998. Expression and decay of two rice class I low molecular mass heat shock protein genes which differ only in 3’ untranslated regions. Plant Physiol. 117: Abstract 768. (*presenter). (NSC 85-2311-B-002-010-B13 & NSC 86-2311-B-002-016-B01).

      Huang CY, PFL Chang*, FC Chang, TS Tseng, and CY Lin 1998. Characterization of a gene, Oshsp16.9C, encoding a 16.9 kDa class I low-molecular-weight heat shock protein in rice. Plant Physiol. 117: Abstract 504. (*presenter). (NSC 84-2311-B-002-007-B01 & NSC 85-2311-B-002-010-B13).

      Chang PFL*, CY Huang, KW Yeh, YM Chen, and CY Lin 1997. A partial cDNA clone from rice (Oryza sativa L.) encoding a class II low-molecular-weight heat shock protein. Plant Physiol. 114: Abstract 850. (*presenter). (NSC 84-2311-B002-007-B01 & NSC 85-2311-B002-010-B13 & NSC 85-2811-B002-006).

      Jinn TL, PFL Chang, and CY Lin* 1997. Immunolocalization of class I low molecular weight heat shock proteins in soybean. Plant Physiol. 114: Abstract 642. (*presenter). (NSC 80-0211-B002-18 & NSC 81-0211-B002-13).

      Chang PFL*, JC Guan, FC Chang, WC Lin, TS Tseng, and CY Lin 1997. Characterization of genes for class I low-molecular weight heat shock proteins in rice. Abstract. The Fifth International Congress of Plant Molecular Biology, Singapore. (*presenter). (NSC 84-2311-B002-030)

      Jinn TL, PFL Chang, and CY Lin* 1997. Tissue type-specific expression of class I low molecular weight heat shock proteins in soybean. Abstract. The Fifth International Congress of Plant Molecular Biology, Singapore. (September 21-27, 1997) (*presenter). (NSC 81-0211-B002-13).

      Yeh CH*, PFL Chang, KW Yeh, WC Lin, YM Chen, and CY Lin 1996. Expression of the rice pTS1 gene encoding 16.9 kD heat shock protein in Escherichia coli. Abstract 003. 1996 Annual Meeting of Chinese Biochem. Society. Taipei. (The 11th Annual Conference of Biomedical Sci.) (*presenter). (NSC 84-2311-B002-007-B01 & NSC 85-2311-B002-010-B13).

      Lin WC*, PFL Chang, KW Yeh, YM Chen, and CY Lin 1996. Studies on the gene expressions of the 16.9 kD heat shock protein genes of rice. Abstract P11. 1996 Annual Meeting of Chinese Biochem. Society. Taipei. (*presenter). (The 11th Annual Conference of Biomedical Sci.) (NSC 84-2311-B002-007-B01).

      Lin CY*, CH Yeh, PFL Chang, KW Yeh, and YM Chen 1996. Expression of a rice gene encoding 16.9 kD heat-shock protein in E. coli enhances thermotolerance. Plant Physiol. 111: Abstract 195. (*presenter). (NSC 84-2311-B002-007-B01 & NSC 85-2311-B002-010-B13).

      Chang PFL*, JC Guan, FC Chang, WC Lin, and CY Lin 1996. Characterization of rice class I low molecular weight heat shock protein genes clustered on chromosome 3. Abstract 3.14. The Fifth International Symposium on Rice Molecular Biology. Taipei. (October 14-15, 1996) (*presenter). (NSC 83-0211-B002-92 & NSC 84-2311-B002-030)

      Yeh CH*, PFL Chang, WC Lin, and CY Lin 1996. Functional analysis of OsHSP 16.9 in E. coli : effect on thermotolerance. Abstract 4.1. The Fifth International Symposium on Rice Molecular Biology. Taipei. (October 14-15, 1996) (*presenter). (NSC 84-2311-B002-007-B01 & NSC 85-2311-B002-010-B13).

      Lin WC*, KW Yeh, PFL Chang, YM Chen, and CY Lin 1995. Study on the genes structure and function of the 16.9 kD heat shock protein of rice. Abstract 187. 1995 Annual Meeting of Chinese Biochem. Society., Taipei. (The Tenth Joint Annual Conference of Biomedical Sci.) (*presenter). (NSC 83-0211-B002-94).

      Chang PFL*, M Reuvani, Y Xu, Z Chen, NK Singh, PM Hasegawa, and RA Bressan 1993. Cloning and characterization of a gene associated with membranes of salt-adapted tobacco cells. Plant Physiol. 102: Abstract 738. (*presenter).

(C)專書及專書論文

          MA Dita, C Waalwijk, P Mutua, A Daly, PFL Chang, BM Corcolon, L Paiva, M Souza, J Kema GH. 2013. Detecting Fusarium oxysporum f. sp. cubense tropical race 4 in soil and symptomless banana tissues. Acta Horticulturae (ISHS) 986: 127-130. (25 April 2013)

          PFL Chang*, YH Lin, CC Chen, YH Lin, YS Lin. 2013. Adjustment of crops to biological stresses. In: Climate-smart Agricultural Production, CY Tai, CS Chang, and CM Yang ed., Taichung District Agricultural Research and Extension Station, Changhua, Taiwan, R.O.C., pp. 79-86.

張碧芳*、林盈宏、陳建志、林奕宏、林昀聖。2013。作物對生物性逆境的調適,pp. 79-86。氣候智能型農作生產,戴振洋、張致盛、楊純明主編,臺中區農業改良場特刊第120號,彰化,台灣,95

          Lin WC and PFL Chang. 2009. Approaches for acquired tolerance to abiotic stress of economically important crops. In: Biocatalysis and Agricultural Biotechnology, CT Hou and JF Shaw eds, CRC Press, Boca Raton, FL, U.S.A., pp. 63-87.

          張碧芳、黃振文、曾國欽 編。2008。植物重要防疫檢疫病害診斷鑑定技術研習會專刊()。行政院農業委員會動植物防疫檢疫局出版,台北,台灣,80頁。

          張碧芳、黃振文、曾國欽 編。2007。植物重要防疫檢疫病害診斷鑑定技術研習會專刊()。行政院農業委員會動植物防疫檢疫局出版,台北,台灣,116頁。

          陳啟予、張碧芳、黃振文、曾國欽 編。20072007年海峽兩岸學術研討會-近代植物病理研究之進展。農委會動植物防檢局出版,中華民國植物病理學會發行,台中,台灣。173頁。

          Wang CL, PFL Chang and PP Ueng2007Genetic diversity and identification of cereal leaf blotch pathogens, Phaeosphaeria species2007年海峽兩岸學術研討會-近代植物病理研究之進展。農委會動植物防檢局出版,中華民國植物病理學會發行,台中,台灣。79-98頁。

          陳啟予、張碧芳、黃振文、楊宏仁 編。2006。作物病害管理技術研討會。行政院農委會動植物防疫檢疫局出版,台北,台灣,223頁。

          張碧芳、黃振文、曾國欽 編。2006。植物重要防疫檢疫病害診斷鑑定技術研習會專刊()。行政院農委會動植物防疫檢疫局出版,台北,台灣,118頁。

      張碧芳、黃振文 編。2005。植物重要防疫檢疫病害診斷鑑定技術研習會專刊()。行政院農委會動植物防疫檢疫局出版,台北,台灣,172頁。

      張碧芳、許靖祺、林盈宏。2004。聚合酶鏈鎖反應。p.225-233。植物基因轉殖與分子檢測技術。葉錫東、陳良築、楊長賢、曾志正、詹富智 編。植物生物技術教學資源中心主編,台中市,台灣。

      張碧芳、許靖祺。2004。反轉錄聚合酶鏈鎖反應。p.235-246。植物基因轉殖與分子檢測技術。葉錫東、陳良築、楊長賢、曾志正、詹富智 編。植物生物技術教學資源中心發行,台中市,台灣。

      張碧芳2004。溫度對作物的影響及熱休克蛋白質的功能。p.119-156。植物重要防疫檢疫病害診斷鑑定技術研習會專刊()張碧芳、黃振文 編。行政院農委會動植物防疫檢疫局出版,台北,台灣。

      張碧芳、黃振文 編。2004。植物重要防疫檢疫病害診斷鑑定技術研習會專刊()。行政院農委會動植物防疫檢疫局出版,台北,台灣,184頁。張碧芳、黃振文、陳隆鐘、林益昇 編。2003。植物病理學會特刊新一號 (新世紀植物病理科技學術研討會專刊)。中華民國植物病理學會出版,台中,台灣,131頁。

      張碧芳2002。鐮胞菌的分子檢測技術以香蕉黃葉病菌為例。p.65-82。植物重要防檢疫疫病診斷鑑定技術研習會專刊。張碧芳、蔡東纂、曾國欽、黃振文編。行政院農委會動植物防疫檢疫局出版,台北,台灣。

      張碧芳2002。鐮胞菌的分子檢測實務以香蕉黃葉病菌為例。p.83-98。植物重要防檢疫疫病診斷鑑定技術研習會專刊。張碧芳、蔡東纂、曾國欽、黃振文編。行政院農委會動植物防疫檢疫局出版,台北,台灣。

      張碧芳、蔡東纂、曾國欽、黃振文 編。2002。植物重要防檢疫疫病診斷鑑定技術研習會專刊。行政院農委會動植物防疫檢疫局出版,台北,台灣,208頁。

      Chang PFL and CY Lin 2000. The discovery of the heat shock response in plants. In: Discoveries in Plant Biology Vol. 3, SD Kung and SF Yang eds. World Scientific Publishing Co. Pte. Ltd., Singapore, pp.347-370.

      Lin CY, CH Yeh, SS Tzeng, TS Tseng, FC Chang, YL Lee, TL Jinn and PFL Chang 1997. Class I low-molecular-weight heat-shock proteins in Oryza sativa. pp 41-52. In: Proceedings of Fifth International Symposium on Rice Molecular Biology.

      Chang PFL 1994. Induction of pathogenesis-related genes and pathogen resistance in tobacco by fungal elicitors and plant growth regulators. Ph.D. Dissertation. Department of Horticulture, Purdue University, West Lafayette, Indiana, USA.

 

(D)研發成果智慧財產權及其應用績效:

專利 (A)發明專利(B)新型專利(C)新式樣專利。

類別

專利名稱

國別

專利號碼

發明人

專利權人

專利期間

國科會計畫編號

B

一種作物種苗抗病性與抗逆境力檢測裝置

中華民國

新型第M273194

陳裕星、洪爭坊、張碧芳

台中區農業改良場

2005-8-212014-12-27

 

A

用於檢測香蕉黃葉病菌之引子、方法及套組

中華民國

新發明第I402346

張碧芳、林盈宏、張景宜、黃振文

國立中興大學

2013.07.212028.05.05

 

 

 

 

A

用於檢測西瓜蔓割病菌之引子、方法及套組

中華民國

新發明第I391490

張碧芳、林盈宏、張景宜、陳甘澍、黃振文

國立中興大學

2013.04.012029.03.31

 

 

 

技術移轉

技術名稱

專利名稱

授權單位

被授權單位

合約期間

國科會計畫編號

作物種苗抗病性與抗逆境力檢測裝置

一種作物種苗抗病性與抗逆境力檢測裝置(中華民國專利-新型第M273194號)

台中區農業改良場

艾特克生物科技股份有限公司 (新台幣八萬元)

2005年1月- 2009年12月

 

奈米物質促進蔬菜種子及幼苗生長的技術

奈米物質促進蔬菜種子及幼苗生長的技術技轉校內編號:K102037

國立中興大學

台茂奈米生化股份有限公司(新台幣三十萬元)

2013.12至2020.12

 

 

其他協助產業技術發展之具體績效

利用快速分子檢測技術協助農業試驗所植病組及種原室、福爾摩莎園藝公司、及台灣香蕉試驗所等單位檢測未知菌株及樣品是否為(或攜帶)香蕉黃葉病菌

利用快速分子檢測技術協助種苗公司與防檢局檢測西瓜種子是否帶有西瓜蔓割病菌

近三年內核定及申請中之研究計畫:

計 畫 名 稱(本會補助者請註明編號)

計畫內擔任之工作


起迄年月

補助或委託機構

申請(核定)情形

經濟部學界開發產業技術計畫補助─研製農業奈米製劑產品提升民生化工產業加值效益3年計畫

101-EC-17-A-21-S1-229

分項計畫主持人

20131月-

201612

經濟部

通過

跨國頂尖研究中心-NCHU-UCD國際植物與食品生物科技中心NSC-102-2911-  I-005-301, NSC-103-2911-I-005-301

研究人員

20122月-

20171

科技部

通過

邁向頂尖計畫--微生物功能基因體解析及其在安全農業之研發與應用

研究人員

20131月-

201512

教育部

通過

利用轉錄基因體定序法分析西瓜抗蔓割病之分子機制   

101-2313-B-005 -028 -MY3

計畫主持人

20128月-

20157

科技部

 

通過

重要作物苗期病害診斷檢測及防治管理技術之研發與應用─利用分子鑑定技術調查台灣香蕉黃葉病菌生理小種之田間分佈

101農科-10.3.1-檢-B3(2)

研究人員

20122月-

201212

 

農委會

動植物防檢局

通過

 

 

 

進行中的研究計劃:

1. 台灣西瓜蔓割病菌之檢測、生理小種鑑定及分佈分析(國科會計畫)

2. 開發西瓜種子蔓割病菌之分子檢測核心技術(國科會產學合作計畫)

3. 開發萵苣萎凋病菌之分子鑑定核心技術(農委會防檢局計畫)

4. 以辣椒為模式探討寄主抗炭疽病之相關基因體分析(農委會防檢局計畫)

目前的研究方向:

1. 開發感染香蕉、西瓜、萵苣及番茄等重要作物尖鐮孢菌及鐮孢菌屬菌株的分子檢測技術

2. 西瓜抗蔓割病之遺傳分析與分子標誌篩選

3. 西瓜抗蔓割病之抗性機制研究

4. 辣椒抗炭疽病之防禦相關基因選殖與定性分析

5. 水稻小分子量熱休克蛋白質與植物病程相關蛋白質之基因表現分析與其其參與在植物抗逆境或抗病之功能研究