分子植物病原真菌研究室


李敏惠 教授

 

重要學經歷

中興大學植物病理系 系主任 (2016-2018)
中興大學/中研院  微生物基因體學博士學位學程主任 (2015 – 2016)
中興大學生物科技發展中心教學推動組 組長 (2012 – 2016) 
中興大學植物病理學系教授 (2012 – 迄今)
美國加州大學戴維斯分校博士 (2005)

聯絡資訊

電話:04-22840780 #343
信箱:mhlee@nchu.edu.tw
辦公室:農環大樓6B04
教師專頁:建置中

 

研究方向與未來發展

本研究室主要探討植物病原真菌與寄主之交互作用及開發生物製劑以防治植物病害,目前研究領域主要有四項:

  1. 辣椒炭疽病菌(Colletotrichum acutatum) 與辣椒之交互作用:利用基因體學、轉錄體學及分子遺傳學方法研究candidate effector proteins 在辣椒炭疽病菌侵染辣椒之功能及與植物交互作用之機制。
  2. 芒果炭疽病菌(C. gloeosporioides)與芒果之交互作用:利用基因體學、轉錄體學及分子遺傳學方法研究芒果炭疽病菌特定基因群對感應寄主因子及環境因子之功能性及作用機制。
  3. 水稻對Pythium 及 Fusarium之抗病基因研究:利用TRIM 基因突變庫進行抗病基因篩選及研究。目前針對已篩選出之抗性基因進行功能分析、抗病機制探討及應用性分析。
  4. 芒果炭疽病及水稻病害之生物性防治劑開發:開發芒果炭疽病菌之生物防治製劑及開發內生菌以防治重要水稻病害。
教授課程

分子植物病理學、分子植物病理學實驗真菌分子遺傳文獻選讀、生物製劑農業循環經濟專題介紹、循環農業之生物精煉技術

期刊論文
  1. Pei-Ling Yu, Chih-Li Wang, Pei-Yin Chen and Miin-Huey Lee* 2017. YAP1 homologue-mediated redox sensing is crucial for a successful infection by Monilinia fructicola. Molecular Plant Pathology 18(6): 783–797. DOI: 10.1111/mpp.12438
  2. Bang-Jau You, Ni Tien, Miin-Huey Lee, Bo-Ying Bao, Yih-Shyuan Wu, Tsung-Chi Hu & Hong-Zin Lee. 2017. Induction of apoptosis and ganoderic acid biosynthesis by cAMP signaling in Ganoderma lucidum. Scientific Reports 7: 318. doi:10.1038/s41598-017-00281-x
  3. Lo, Shuen-Fang; Ho, Tuan-Hua; Liu, Yi-Lun; Jiang, Miring-Jier; Hsieh, KunTing; Chen, Ku-Ting; Yu, Lin-Chih; Lee, Miin-Huey; Chen, Chi-yu; Huang, Tzu-Pi; Kojima, Mikoko; Sakakibara, Hitoshi; Chen, Liang-Jwu; Yu, Su-May (2016, Dec). Ectopic expression of specific GA2 oxidase mutants promotes yield and stress tolerance in rice. Plant Biotechnology. DOI: 10.1111/pbi.12681
  4. T. Liu., K. D., Chiu, T. K., Hsieh, J. W., Huang and M. H. Lee* 2015. Development of detached mango leaf-inoculation system for rapid evaluating the efficacy of biocontrol agent on controlling mango anthracnose. Plant Pathol. Bull. 24: 189-199.
  5. Chien-Ming Chou, Fang-Yi Yu, Pei-Ling Yu, Richard M. Bostock, Kuang-Ren Chung, Jenn-Wen Huang and Miin-Huey Lee*. 2015. Expression of five endopolygalacturonase genes and demonstration that MfPG1 overexpression diminishes virulence in the brown rot pathogen Monilinia fructicola. PLoS One 10(6):e0132012.
  6. Chiu-Min Chiu, Bang-Jau You, Chien-Ming Chou, Pei-Ling Yu, Fang-Yi Yu, Shiah-Mei Pan, Richard M. Bostock, Kuang-Ren Chung and Miin-Huey Lee*. 2013. Redox status-mediated regulation of gene expression and virulence in the brown rot pathogen Monilinia fructicola. Plant Pathology, 62: 809-819
  7. De Cal, P. Sandín-Espa˜na, F. Martinez, B. Egüen, C. M. Chiou, M.H. Lee, P. Melgarejoa and D. Prusky. 2013. Role of gluconic acid and pH modulation in virulence of Monilinia fructicola on peach fruit. Postharvest Biology and Technology 86: 418–423.
  8. Bang-Jau You*, Miin-Huey Lee, Ni Tien, Meng-Shiou Lee, Hui-Chuan Hsieh, Lin-Hsien Tseng, Yu-Lin Chung, and Hong-Zin Lee. 2013. A Novel Approach to Enhancing Ganoderic Acid Production by Ganoderma lucidum Using Apoptosis Induction. PLoS ONE 8(1):
  9. Bang-Jau You, Hong-Zin Lee, Kuang-Ren Chung, Miin-Huey Lee , Mei-Jung Huang, Ni Tien, Chiao-Wei Chan and Yueh-Hsiung Kuo. 2012. Enhanced production of ganoderic acids and cytototoxicity activity by Ganoderma lucidum using solid-medium culture. Bioscience Biotechnology and Biochemistry 76:120270-1-6. IF: 2.292
  10. Miin-Huey Lee*, Shiah-Mei Pan, Thieng-Wei Ngu, Po-Sheng Chen, Li-Yuan Wang and Kuang-Ren Chung. 2011. Mutations of β-tubulin codon 198 or 200 indicate thiabendazole resistance among isolates of Penicillium digitatum collected from citrus in Taiwan. International Journal of Food Microbiology 150(2-3): 157-163
  11. Po-Sheng Chen, Li-Yuan Wang, You-Jen Chen, Kuo-Ching Tzeng, Shih-Chieh Chang, Kuo-Ching Tzeng, Kuang-Ren Chung* and Miin-Huey Lee*. 2012. Understanding cellular defence in kumquat and calamondin to citrus canker caused by Xanthomonas citri citri. Physiological and Molecular Plant Pathology. 79: 1-12 (IF:1.377, 96/190=50.5% in Plant Science)
  12. Chien-Yao Liao, Mei-Ya Chen, Yu-Ken Chen, Tien-Cheng Wang Zong-Ming Sheu, Ker-Chung Kuo, Pi-Fang Chang, Kuang-Ren Chung and Miin-Huey Lee*. 2012. Characterization of three Colletotrichum acutatum isolates from Capsicum European Journal of Plant Pathology 133:599-608
  13. Chien-Yao Liao, Mei-Ya Chen, Yu-Ken Chen, Ker-Chung Kuo, Kung-Ren Chung and Miin-Huey Lee*. Formation of highly branched hyphae by Colletotrichum acutatum within the fruit cuticles of Capsicum spp. Plant Pathology 61: 262–270
  14. Miin-Huey Lee, Chiu-Min Chiu, Tatiana Roubtsova, Chien-Ming Chou and Richard M. Bostock*. 2010. Overexpression of a redox-regulated cutinase gene MfCUT1 increases virulence of the brown rot pathogen Monilinia fructicola on Prunus Molecular Plant-Microbe Interactions. 23:176-186
  15. You, B. J., Lee, M. H. and Chung, K.R.Gene-specific disruption in the filamentous fungus Cercospora nicotianae using a split-marker approach. Archive Microbiology 191:615-622.
  16. You, B. J., Lee, M. H. and Chung, K.R. Production of cercosporin toxin by the phytopathogenic Cercospora fungi is affected by diverse environmental signals. Canadian Journal of Microbiology 54: 259-269
  17. Chen, H. Q., Lee, M. H., and Chung, K. R. 2007. Functional characterization of three genes encoding putative oxidoreductases required for cercosporin toxin biosynthesis in the fungus Cercospora nicotianae. Microbiology 153:2781-2790
  18. Chen, H. Lee, M. H., Daub, M. E., and Chung, K. R. 2007. Molecular analysis of the cercosporin biosynthetic gene cluster in Cercospora nicotianae. Molecular Microbiology 64(3): 755–770
  19. Choquer, M., Lee, M. H., Bau, H. J., and Chung, K. R. Deletion of a MFS transporter-like gene in Cercospora nicotianae reduces cercosporin toxin accumulation and fungal virulence. FEBS Letters 581:489-494
  20. Dekkers, K. L., You, B. J., Chen, H. Q., Gowda, V. S., Liao, H. L., Lee, M. H., Ueng, P. P. and Chung K. R. 2007. The Cercospora nicotianae gene encoding dual O-methyltransferase and FAD-dependent monooxygenase domains mediates cercosporin biosynthesis. Fungal genetic and biology 44(5):444-54
  21. Lee, M. H. and Bostock, R. M. 2007. Fruit exocarp phenols in relation to quiescence and development of Monilinia fructicola infections in Prunus: a role for cellular redox. Phytopathology 97:269-277
  22. Lee, M. H. and Bostock, R. M. 2006. Induction, regulation, and role in pathogenesis of appressoria in Monilinia fructicola. Phytopathology 96:1072-1080
  23. Lee, M. H. and Bostock, R. M. 2006. Agrobacterium T-DNA-mediated integration and gene replacement in the brown rot pathogen Monilinia fructicola. Current Genetics 49:309-322
  24. Lee, M. H.*, Tzeng, D. D. S. and Hsu, F. S. 2006. Photodynamic effects of methionine-riboflavin mixture on antioxidant proteins. Plant Pathol. Bull. 15:17-24
  25. Lee, M. H.* and Tzeng, D. D. S. 2006. Pantoea agglomerans is a potential pathogen causing necrosis on mongo leaf. Plant Pathol. Bull. 15:63-68
論文專書
  1. Kuang-Ren Chung and Miin-Huey Lee. Split marker-mediated transformation and targeted gene disruption in filamentous fungi (invited contribution). Genetic Transformation System in Fungi, Volume 2. (ISBN: ISBN 978-3-319-10503-1 (eBook)). Springer Publishes. Dec, 2014: Chapter 15, p. 175-180.
  2. Lee, M. H. 2005. Microscopic, physiological and molecular studies of pathogenesis in Monilinia fructicola, the brown rot pathogen of stone fruits. Ph.D. dissertation. University of California, Davis, USA.
獲獎
  1. 獲得美國加州大學植病系所頒發的William J. Moller Graduate Student Research Award (2006)獎狀及獎金
研討會論文
  1. Miin-Huey Lee,*, Kuan-Ting, Liu, and Jenn-Wen Huang. Development of bio-based strategies to control anthracnose disease on mango. The 8th International Conference of Clinical Plant Science. Dec. 11, Daegu, Korea. (Invited speech)
  2. Miin-Huey Lee*, Chia-Chi Kuo, Yung-Chu Lin, Meng-Yi Lin, Shu-Cheng Chuang, and Ming-Che Shih. 2017. Reverse and forward genetic studies for the pathogenicity and growth of Colletotrichum. 2017 Taiwan-Japan Mycological Research Conference. Oct. 21, Taipei, Taiwan. (Invited speech)
  3. Chia-Chi Chang, Kuan-Ting Liu, Jenn-Wen Huang, and Miin-Huey Lee*, 2016. Mango Anthracnose: Pathogenicity and Disease Control. International Symposium on Agricultural Biotechnology, Dec. 12, 2016, NCHU, Taichung, Taiwan. (Invited speech)
  4. Chia-Chi Chang, Yung-Chu Lin, Miin-Huey Lee*. 2016. Agrobacterium T-DNA insertions identified genes involved in growth and pathogenicity of Colletotrichum gloeosporioides on mango. 13th European Conference on Fungal Genetics, April 3-6, Paris, France.
  5. Pei-Ling Yu, Chia-Chi Chang, Chiu-Min Chiu, Pei-Yin Chen and Miin-Huey Lee* 2016. The transcription factor MfAP1-mediated redox sensing is crucial for a successful infection by Monilinia fructicola. 13th European Conference on Fungal Genetics, April 3-6, Paris, France.
  6. Kuan-Ting Liu, Jenn-Wen Huang and Miin-Huey Lee*. 2015. Screening and application of antagonistic bacteria and plant extracts to control anthracnose disease on mango. International Symposium on Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus and Bangkok Campus, Thailand, Dec. 16-20. (Invited speech)
  7. Miin-Huey Lee. 2015. Molecular mechanisms involved in the interaction of Monilinia fructicola and Rosaceae. Monilinia Workshop, Nov. 25-26, Lleida, Spain, 2015. (Invited speech)
  8. Miin-Huey Lee*, Shiah-Mei Pan, Dai-Kang Hsieh, Chiu-Min Chiu, and Chia-Chi Chang. 2015. Identification and detection of peach brown rot pathogens in Taiwan. 6th International Conference of Clinical Plant Science 2015, Tokyo University, Tokyo, Japan, Dec. 5.
  9. Fang-Yi Yu, Chiu-Min Chiu, Shiow-Ju Lee, Maw-Rong Lee and Miin-Huey Lee*. 2015. Antibiotic activity of the stone fruit pathogen Monilinia fructicola: characterization and identification. 11th International symposium on Biocatalysis and Agricultural Biotechnoloy, Banff National Park, Alberta, Canada, Sept. 13-16. (Invited speech)
  10. Miin-Huey Lee, Chein-Yao Liao, Mei-Ya Chen, Yung-Chu Lin and Jia-Fang Ho. 2014. Pathogenicity of Colletotrichum acutatum on chili pepper. International Symposium for Agricultural Biotechnology, Hanoi, Vietnam, Dec. 3-9. (Invited speech)
  11. Miin-Huey Lee, Pei-Ling Yu, Chiu-Min Chiu, Fang-Yi Yu, and Richard M. Bostock. 2014. The interactions of Monilinia fructicola and Rosaceae: Redox and 10th International symposium on Biocatalysis and Agricultural Biotechnoloy, Kaohsiung, Taiwan, Oct. 28-31 (Invited speech)
  12. Chiu-Min Chiu, Fang-Yi Yu, Richard M. Bostock* and Miin-Huey Lee. 2014. Functional analysis of genes encoding polyketide synthase in Monilinia fructicola, a causal agent of peach brown rot. 10th International symposium on Biocatalysis and Agricultural Biotechnoly, Kaohsiung, Taiwan, Oct. 28-31
  13. Shu-Cheng Chuang, Miin-Huey Lee and Ming-Che Shih. 2014. Genomic and transcriptomic analyses unravel effector repertoire of Colletotrichum acutatum during the infection on Capsicum annuum. 12th European Conference of Fungal Genetics. Seville, Spain, March 23-27
  14. Bostock RM, Liu T-H, Yaghmour M, Leveau J, Lee M-H, Martinez-Garcia PJ, Parfitt DE, Gradziel TM, Crisosto CH. 2014. Brown rot in stone fruits, redox and resistance. Invited abstract and speaker, XVII Congress of the Spanish Society for Phytopathology, Lleida, Spain, October 7-10, 2014. Publications Page 33 of 39
  15. Liu T-H, Yaghmour MA, Lee MH, Leveau J, Bostock RM. 2013. Construction and expression of roGFP in Pantoea agglomerans as a bioreporter for host surface redox potential in plant-microbe interactions. Phytopathology, 103: 84-85.)
  16. Fang-Yi Yu, Chien-Ming Chou, Pei-Ling Yu, Kuang-Ren Chung, Richard M. Bostock and Miin-Huey Lee*.2013. Differential expression of five endopolygalacturonase genes and functional demonstration of MfPG1 as a negative virulence determinant in the brown rot pathogen Monilinia fructicola. XI International Fungal Biology Conference. Karlsruhe, Germany, September 29 – October 3.
  17. Fang-Yi Yu, Chien-Ming Chou, Pei-Ling Yu, Kuang-Ren Chung, Richard M. Bostock and Miin-Huey Lee*.2013. Differential expression of five endopolygalacturonase genes and functional demonstration of MfPG1 as a negative virulence determinant in the brown rot pathogen Monilinia fructicola. XI International Fungal Biology Conference. Karlsruhe, Germany, September 29 – October 3.
  18. Miin-Huey Lee. 2012. The interactions of Monilinia fructicola and peach: redox, pathogenicity and polyketides. Monilinia Workshop, Dec. 13-14, Lleida, Spain, 2012. (Invited speech)
  19. Pei-Ling Yu, Pei-Yin Chen and Miin-Huey Lee*. 2012. Functional analysis of an oxidative stress-regulated gene MfAP1 from Monilinia fructicola. XV International Congress of Molecular Plant-Microbe Interactions, July 29- August 2, Kyoto, Japan. IS-MPMI 2012 XV International Congress of Molecular Plant-Microbe Interactions program and abstracts, PS04-222.
  20. Mei-Ya Chen and Miin-Huey Lee*. 2012. Induction and regulation of highly branched penetration structure of Colletotrichum acutatum. XV International Congress of Molecular Plant-Microbe Interactions, July 29- August 2, Kyoto, Japan. IS-MPMI 2012 XV International Congress of Molecular Plant-Microbe Interactions program and abstracts, PS04-223
  21. Chien-Yao Liao and Miin-Huey Lee*. 2012. Formation of highly branched hyphae by Colletotrichum acutatum within the fruit cuticles of Capsicum XV International Congress of Molecular Plant-Microbe Interactions, July 29- August 2, Kyoto, Japan. IS-MPMI 2012 XV International Congress of Molecular Plant-Microbe Interactions program and abstracts, PS04-221.
  22. Fang-Yi Yu, Chien-Min Chiou, Pei-Ling Yu, Richard M. Bostock, and Miin-Huey Lee*. 2011.Antimicrobial activity and polyketide synthase gene cloning of the stone fruit pathogen Monilinia fructicola. 26th Fungal Genetic Conference, March 15-20, 2011, Asilomar, CA. Fungal Genetics reports, vol.58-supplement, abstract No. 27 
指導研究生及獲獎情形
  1. 指導大學部洪譽芳獲得科技部大專生專題研究計畫補助 (2017 – 2018)。
  2. 指導研究生陳原諄獲得「2016中華民國植物病理學會年會」,學生口頭發表論文競賽乙組第二名。
  3. 指導研究生郭家琪獲得「2015中華民國植物病理學會年會」,學生口頭發表論文競賽乙組第一名。
  4. 指導研究生邱秋閔參加2014國際研討會ISBAB (International symposium on Biocatalysis and Agricultural Biotechnoloy) 海報競賽獲得優良研究獎。
  5. 指導研究生獲得「2010中華民國植物病理學會年會」,學生口頭發表論文競賽第一名(廖建堯)及第二名(周建銘)。
  6. 指導研究生邱秋閔參加美國植物病理年會(APS, 2009)發表論文獲得國科會研究生出國發表論文經費補助。

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