分子植物病原真菌研究室 — _英文版

Fungal Genetics and Molecular Biology; Plant-Microbe Interactions; Bio-agents for Disease Management

Research in my Lab focuses on fungal pathogen-host interactions and disease management with the development of bio-agents.  Current projects include:

(1) Molecular interactions of  Colletotrichum acutatum and chili pepper:  We use genomic, transcrptomic and genetic approaches to analyze the function of candidate effector proteins. We also establish a T-DNA insertion library to identify novel fungal development and/or pathogenicity genes.

(2) Molecular interactions of  Colletotrichum gloeosporioides and mango:  We use genomic, transcrptomic and genetic approaches to identify potential genes involved in fungal pathogenicity and sensing to host factor and various environmental stresses.

(3) Disease resistance in rice to Pythium and Fusarium:  We have identified several potential genes in rice which are involved in  resistance to Pythium and Fusarium pathogens. Currently we are working on characterizing their function, mechanisms and applications.

(4) Disease management with bio-agents: We have developed two biocontrol agents to control mango anthracnose disease and currently are working on commercialization of the two bio-agents.  We are developing several potential endophytes to control Pythium and Fusarium pathogens in rice by collaboration with  Prof. Li-Sen Young, National Formosa University/Department of Biotechnology in Taiwan.

Molecular Plant Pathology, Laboratory of Molecular Plant Pathology, Biopesticides 

1. Pei-Ling Yu, Chih-Li Wang, Pei-Yin Chen and Miin-Huey Lee* 2017. YAP1 homologue-mediated redox sensing is crucial for a successful infection by Monilinia fructicola. Molecular Plant Pathology 18(6): 783–797. DOI: 10.1111/mpp.12438
2. Bang-Jau You, Ni Tien, Miin-Huey Lee, Bo-Ying Bao, Yih-Shyuan Wu, Tsung-Chi Hu & Hong-Zin Lee. 2017. Induction of apoptosis and ganoderic acid biosynthesis by cAMP signaling in Ganoderma lucidum. Scientific Reports 7: 318. doi:10.1038/s41598-017-00281-x
3. Lo, Shuen-Fang; Ho, Tuan-Hua; Liu, Yi-Lun; Jiang, Miring-Jier; Hsieh, KunTing; Chen, Ku-Ting; Yu, Lin-Chih; Lee, Miin-Huey; Chen, Chi-yu; Huang, Tzu-Pi; Kojima, Mikoko; Sakakibara, Hitoshi; Chen, Liang-Jwu; Yu, Su-May (2016, Dec). Ectopic expression of specific GA2 oxidase mutants promotes yield and stress tolerance in rice. Plant Biotechnology. DOI: 10.1111/pbi.12681
4. T. Liu., K. D., Chiu, T. K., Hsieh, J. W., Huang and M. H. Lee* 2015. Development of detached mango leaf-inoculation system for rapid evaluating the efficacy of biocontrol agent on controlling mango anthracnose. Plant Pathol. Bull. 24: 189-199.
5. Chien-Ming Chou, Fang-Yi Yu, Pei-Ling Yu, Richard M. Bostock, Kuang-Ren Chung, Jenn-Wen Huang and Miin-Huey Lee*. 2015. Expression of five endopolygalacturonase genes and demonstration that MfPG1 overexpression diminishes virulence in the brown rot pathogen Monilinia fructicola. PLoS One 10(6):e0132012.
6. Chiu-Min Chiu, Bang-Jau You, Chien-Ming Chou, Pei-Ling Yu, Fang-Yi Yu, Shiah-Mei Pan, Richard M. Bostock, Kuang-Ren Chung and Miin-Huey Lee*. 2013. Redox status-mediated regulation of gene expression and virulence in the brown rot pathogen Monilinia fructicola. Plant Pathology, 62: 809-819
7. De Cal, P. Sandín-Espa˜na, F. Martinez, B. Egüen, C. M. Chiou, M.H. Lee, P. Melgarejoa and D. Prusky. 2013. Role of gluconic acid and pH modulation in virulence of Monilinia fructicola on peach fruit. Postharvest Biology and Technology 86: 418–423.
8. Bang-Jau You*, Miin-Huey Lee, Ni Tien, Meng-Shiou Lee, Hui-Chuan Hsieh, Lin-Hsien Tseng, Yu-Lin Chung, and Hong-Zin Lee. 2013. A Novel Approach to Enhancing Ganoderic Acid Production by Ganoderma lucidum Using Apoptosis Induction. PLoS ONE 8(1):
9. Bang-Jau You, Hong-Zin Lee, Kuang-Ren Chung, Miin-Huey Lee , Mei-Jung Huang, Ni Tien, Chiao-Wei Chan and Yueh-Hsiung Kuo. 2012. Enhanced production of ganoderic acids and cytototoxicity activity by Ganoderma lucidum using solid-medium culture. Bioscience Biotechnology and Biochemistry 76:120270-1-6. IF: 2.292
10. Miin-Huey Lee*, Shiah-Mei Pan, Thieng-Wei Ngu, Po-Sheng Chen, Li-Yuan Wang and Kuang-Ren Chung. 2011. Mutations of β-tubulin codon 198 or 200 indicate thiabendazole resistance among isolates of Penicillium digitatum collected from citrus in Taiwan. International Journal of Food Microbiology 150(2-3): 157-163
11. Po-Sheng Chen, Li-Yuan Wang, You-Jen Chen, Kuo-Ching Tzeng, Shih-Chieh Chang, Kuo-Ching Tzeng, Kuang-Ren Chung^* and Miin-Huey Lee*. 2012. Understanding cellular defence in kumquat and calamondin to citrus canker caused by Xanthomonas citri citri. Physiological and Molecular Plant Pathology. 79: 1-12 (IF:1.377, 96/190=50.5% in Plant Science)
12. Chien-Yao Liao, Mei-Ya Chen, Yu-Ken Chen, Tien-Cheng Wang Zong-Ming Sheu, Ker-Chung Kuo, Pi-Fang Chang, Kuang-Ren Chung and Miin-Huey Lee*. 2012. Characterization of three Colletotrichum acutatum isolates from Capsicum European Journal of Plant Pathology 133:599-608
13. Chien-Yao Liao, Mei-Ya Chen, Yu-Ken Chen, Ker-Chung Kuo, Kung-Ren Chung and Miin-Huey Lee*. Formation of highly branched hyphae by Colletotrichum acutatum within the fruit cuticles of Capsicum spp. Plant Pathology 61: 262–270
14. Miin-Huey Lee, Chiu-Min Chiu, Tatiana Roubtsova, Chien-Ming Chou and Richard M. Bostock*. 2010. Overexpression of a redox-regulated cutinase gene MfCUT1 increases virulence of the brown rot pathogen Monilinia fructicola on Prunus Molecular Plant-Microbe Interactions. 23:176-186
15. You, B. J., Lee, M. H. and Chung, K.R.Gene-specific disruption in the filamentous fungus Cercospora nicotianae using a split-marker approach. Archive Microbiology 191:615-622.
16. You, B. J., Lee, M. H. and Chung, K.R. Production of cercosporin toxin by the phytopathogenic Cercospora fungi is affected by diverse environmental signals. Canadian Journal of Microbiology 54: 259-269
17. Chen, H. Q., Lee, M. H., and Chung, K. R. 2007. Functional characterization of three genes encoding putative oxidoreductases required for cercosporin toxin biosynthesis in the fungus Cercospora nicotianae. Microbiology 153:2781-2790
18. Chen, H. Lee, M. H., Daub, M. E., and Chung, K. R. 2007. Molecular analysis of the cercosporin biosynthetic gene cluster in Cercospora nicotianae. Molecular Microbiology 64(3): 755–770
19. Choquer, M., Lee, M. H., Bau, H. J., and Chung, K. R. Deletion of a MFS transporter-like gene in Cercospora nicotianae reduces cercosporin toxin accumulation and fungal virulence. FEBS Letters 581:489-494
20. Dekkers, K. L., You, B. J., Chen, H. Q., Gowda, V. S., Liao, H. L., Lee, M. H., Ueng, P. P. and Chung K. R. 2007. The Cercospora nicotianae gene encoding dual O-methyltransferase and FAD-dependent monooxygenase domains mediates cercosporin biosynthesis. Fungal genetic and biology 44(5):444-54
21. Lee, M. H. and Bostock, R. M. 2007. Fruit exocarp phenols in relation to quiescence and development of Monilinia fructicola infections in Prunus: a role for cellular redox. Phytopathology 97:269-277
22. Lee, M. H. and Bostock, R. M. 2006. Induction, regulation, and role in pathogenesis of appressoria in Monilinia fructicola. Phytopathology 96:1072-1080
23. Lee, M. H. and Bostock, R. M. 2006. Agrobacterium T-DNA-mediated integration and gene replacement in the brown rot pathogen Monilinia fructicola. Current Genetics 49:309-322
24. Lee, M. H.*, Tzeng, D. D. S. and Hsu, F. S. 2006. Photodynamic effects of methionine-riboflavin mixture on antioxidant proteins. Plant Pathol. Bull. 15:17-24
25. Lee, M. H.* and Tzeng, D. D. S. 2006. Pantoea agglomerans is a potential pathogen causing necrosis on mongo leaf. Plant Pathol. Bull. 15:63-68

1. Kuang-Ren Chung and Miin-Huey Lee. Split marker-mediated transformation and targeted gene disruption in filamentous fungi (invited contribution). Genetic Transformation System in Fungi, Volume 2. (ISBN: ISBN 978-3-319-10503-1 (eBook)). Springer Publishes. Dec, 2014: Chapter 15, p. 175-180.
2. Lee, M. H. 2005. Microscopic, physiological and molecular studies of pathogenesis in Monilinia fructicola, the brown rot pathogen of stone fruits. Ph.D. dissertation. University of California, Davis, USA.

1. William J. Moller Graduate Student Research Award (2006), UC Davis

1. Miin-Huey Lee^,*, Kuan-Ting, Liu, and Jenn-Wen Huang. Development of bio-based strategies to control anthracnose disease on mango. The 8^th International Conference of Clinical Plant Science. Dec. 11, Daegu, Korea. (Invited speech)
2. Miin-Huey Lee*, Chia-Chi Kuo, Yung-Chu Lin, Meng-Yi Lin, Shu-Cheng Chuang, and Ming-Che Shih. 2017. Reverse and forward genetic studies for the pathogenicity and growth of Colletotrichum. 2017 Taiwan-Japan Mycological Research Conference. Oct. 21, Taipei, Taiwan. (Invited speech)
3. Chia-Chi Chang, Kuan-Ting Liu, Jenn-Wen Huang, and Miin-Huey Lee*, 2016. Mango Anthracnose: Pathogenicity and Disease Control. International Symposium on Agricultural Biotechnology, Dec. 12, 2016, NCHU, Taichung, Taiwan. (Invited speech)
4. Chia-Chi Chang, Yung-Chu Lin, Miin-Huey Lee*. 2016. Agrobacterium T-DNA insertions identified genes involved in growth and pathogenicity of Colletotrichum gloeosporioides on mango. 13^th European Conference on Fungal Genetics, April 3-6, Paris, France.
5. Pei-Ling Yu, Chia-Chi Chang, Chiu-Min Chiu, Pei-Yin Chen and Miin-Huey Lee* 2016. The transcription factor MfAP1-mediated redox sensing is crucial for a successful infection by Monilinia fructicola. 13^th European Conference on Fungal Genetics, April 3-6, Paris, France.
6. Kuan-Ting Liu, Jenn-Wen Huang and Miin-Huey Lee^*. 2015. Screening and application of antagonistic bacteria and plant extracts to control anthracnose disease on mango. International Symposium on Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus and Bangkok Campus, Thailand, Dec. 16-20. (Invited speech)
7. Miin-Huey Lee. 2015. Molecular mechanisms involved in the interaction of Monilinia fructicola and Rosaceae. Monilinia Workshop, Nov. 25-26, Lleida, Spain, 2015. (Invited speech)
8. Miin-Huey Lee^*, Shiah-Mei Pan, Dai-Kang Hsieh, Chiu-Min Chiu, and Chia-Chi Chang. 2015. Identification and detection of peach brown rot pathogens in Taiwan. 6th International Conference of Clinical Plant Science 2015, Tokyo University, Tokyo, Japan, Dec. 5.
9. Fang-Yi Yu, Chiu-Min Chiu, Shiow-Ju Lee, Maw-Rong Lee and Miin-Huey Lee*. 2015. Antibiotic activity of the stone fruit pathogen Monilinia fructicola: characterization and identification. 11th International symposium on Biocatalysis and Agricultural Biotechnoloy, Banff National Park, Alberta, Canada, Sept. 13-16. (Invited speech)
10. Miin-Huey Lee, Chein-Yao Liao, Mei-Ya Chen, Yung-Chu Lin and Jia-Fang Ho. 2014. Pathogenicity of Colletotrichum acutatum on chili pepper. International Symposium for Agricultural Biotechnology, Hanoi, Vietnam, Dec. 3-9. (Invited speech)
11. Miin-Huey Lee, Pei-Ling Yu, Chiu-Min Chiu, Fang-Yi Yu, and Richard M. Bostock. 2014. The interactions of Monilinia fructicola and Rosaceae: Redox and 10th International symposium on Biocatalysis and Agricultural Biotechnoloy, Kaohsiung, Taiwan, Oct. 28-31 (Invited speech)
12. Chiu-Min Chiu, Fang-Yi Yu, Richard M. Bostock^* and Miin-Huey Lee. 2014. Functional analysis of genes encoding polyketide synthase in Monilinia fructicola, a causal agent of peach brown rot. 10th International symposium on Biocatalysis and Agricultural Biotechnoly, Kaohsiung, Taiwan, Oct. 28-31
13. Shu-Cheng Chuang, Miin-Huey Lee and Ming-Che Shih. 2014. Genomic and transcriptomic analyses unravel effector repertoire of Colletotrichum acutatum during the infection on Capsicum annuum. 12^th European Conference of Fungal Genetics. Seville, Spain, March 23-27
14. Bostock RM, Liu T-H, Yaghmour M, Leveau J, Lee M-H, Martinez-Garcia PJ, Parfitt DE, Gradziel TM, Crisosto CH. 2014. Brown rot in stone fruits, redox and resistance. Invited abstract and speaker, XVII Congress of the Spanish Society for Phytopathology, Lleida, Spain, October 7-10, 2014. Publications Page 33 of 39
15. Liu T-H, Yaghmour MA, Lee MH, Leveau J, Bostock RM. 2013. Construction and expression of roGFP in Pantoea agglomerans as a bioreporter for host surface redox potential in plant-microbe interactions. Phytopathology, 103: 84-85.)
16. Fang-Yi Yu, Chien-Ming Chou, Pei-Ling Yu, Kuang-Ren Chung, Richard M. Bostock and Miin-Huey Lee*.2013. Differential expression of five endopolygalacturonase genes and functional demonstration of MfPG1 as a negative virulence determinant in the brown rot pathogen Monilinia fructicola. XI International Fungal Biology Conference. Karlsruhe, Germany, September 29 – October 3.
17. Fang-Yi Yu, Chien-Ming Chou, Pei-Ling Yu, Kuang-Ren Chung, Richard M. Bostock and Miin-Huey Lee*.2013. Differential expression of five endopolygalacturonase genes and functional demonstration of MfPG1 as a negative virulence determinant in the brown rot pathogen Monilinia fructicola. XI International Fungal Biology Conference. Karlsruhe, Germany, September 29 – October 3.
18. Miin-Huey Lee. 2012. The interactions of Monilinia fructicola and peach: redox, pathogenicity and polyketides. Monilinia Workshop, Dec. 13-14, Lleida, Spain, 2012. (Invited speech)
19. Pei-Ling Yu, Pei-Yin Chen and Miin-Huey Lee*. 2012. Functional analysis of an oxidative stress-regulated gene MfAP1 from Monilinia fructicola. XV International Congress of Molecular Plant-Microbe Interactions, July 29- August 2, Kyoto, Japan. IS-MPMI 2012 XV International Congress of Molecular Plant-Microbe Interactions program and abstracts, PS04-222.
20. Mei-Ya Chen and Miin-Huey Lee*. 2012. Induction and regulation of highly branched penetration structure of Colletotrichum acutatum. XV International Congress of Molecular Plant-Microbe Interactions, July 29- August 2, Kyoto, Japan. IS-MPMI 2012 XV International Congress of Molecular Plant-Microbe Interactions program and abstracts, PS04-223
21. Chien-Yao Liao and Miin-Huey Lee*. 2012. Formation of highly branched hyphae by Colletotrichum acutatum within the fruit cuticles of Capsicum XV International Congress of Molecular Plant-Microbe Interactions, July 29- August 2, Kyoto, Japan. IS-MPMI 2012 XV International Congress of Molecular Plant-Microbe Interactions program and abstracts, PS04-221.
22. Fang-Yi Yu, Chien-Min Chiou, Pei-Ling Yu, Richard M. Bostock, and Miin-Huey Lee*. 2011.Antimicrobial activity and polyketide synthase gene cloning of the stone fruit pathogen Monilinia fructicola. 26^th Fungal Genetic Conference, March 15-20, 2011, Asilomar, CA. Fungal Genetics reports, vol.58-supplement, abstract No. 27

1. 指導大學部洪譽芳獲得科技部大專生專題研究計畫補助 (2017 – 2018)。
2. 指導研究生陳原諄獲得「2016中華民國植物病理學會年會」，學生口頭發表論文競賽乙組第二名。
3. 指導研究生郭家琪獲得「2015中華民國植物病理學會年會」，學生口頭發表論文競賽乙組第一名。
4. 指導研究生邱秋閔參加2014國際研討會ISBAB (International symposium on Biocatalysis and Agricultural Biotechnoloy) 海報競賽獲得優良研究獎。
5. 指導研究生獲得「2010中華民國植物病理學會年會」，學生口頭發表論文競賽第一名(廖建堯)及第二名(周建銘)。
6. 指導研究生邱秋閔參加美國植物病理年會(APS, 2009)發表論文獲得國科會研究生出國發表論文經費補助。